View Article

Abstract

In compared to prior approaches, HPLC provides far higher resolution, more accurate quantitative results, and quicker analysis durations. As a result, HPLC has developed into a vital instrument in many analytical labs. Troubleshooting is a type of problem solving that is frequently used to fix failing processes or goods. Actually, the term "HPLC" is used to describe a variety of separation methods that employ a liquid mobile phase, or eluent. Understanding the fundamental principles underlying the operation of the instrument and the separation is necessary for troubleshooting HPLC equipment and separations. Component faults (pump, degasser, injector, detector, data system, column) and improper mobile phase or sample preparation are the main causes of HPLC issues. For best results, always prepare food in clean glasses. Cleaning the HPLC with the proper solvent is the primary solution to HPLC issues. It is advised to do a fast visual inspection of the instrument and column if any issue arises. Try to follow the same procedure if a monograph or other protocol is supplied. Troubleshooting is a type of issue resolution that is frequently used to fix failing processes or goods. Alternatively, we advise you to read the complete article to learn some tips that will enable you to prevent issues in the future. We hope that this article will help you detect issues and understand their underlying causes so that you can stop or lessen their occurrence in the future. The most crucial aspect of troubleshooting is the care of the colon and HPLC. We attempted to address all significant troubleshooting issues in this post.

Keywords

HPLC troubleshooting, Column Storage & Cleaning, Basic of HPLC

Reference

  1. High performance liquid chromatography troubleshooting. https://www.pharmatutor.org/articles/troubleshootingin-high-performance-liquid-chromatography-hplc (accessed 4th June 2020).
  2. International Journal of Pharma Research and Health Sciences. 2020; 8 (4): 3195-202 3202 IIIIIIIII. Reserved rights 2. Only a top-notch medication development procedure can have an international impact. https://www.waters.com/nextgen/us/en.html (Accessed: 6th June 2020). 
  3. The Common Issues with HPLC Columns and Solutions. Common issues with HPLC columns and solutions may be found at develosil.us. (Accessed 16th June 2020). 
  4. A troubleshooting manual for HPLC. Hplc Troubleshooting Guide at https://www.sigmaaldrich.com/technical-documents/articles/analytical.html (Accessed 20th June 2020). 
  5. HPLC Troubleshooting hints and tips. https://www.chromservis.eu/c/hplc-troubleshooting- Hints and tips - Chromatography (Accessed18th June 2020). 
  6. Johan Dolan's HPLC Troubleshooting Guide is available online at www.ace-hplc.com. (Accessed on18 June 2020). 
  7. Troubleshooting HPLC System Tips & Tricks. Tips & Tricks for HPLC Troubleshooting, available at https://www.agilent.com/cs/library/slidepresentation/Public.pdf (Accessed on14th June 2020). 
  8.  Chromatography Blog. Crawford Scientific Online (Accessed on 17th June 2020). 
  9. Troubleshooting Guide HPLC by Armelle Vallat, pbf.unizg.hr (Accessed on16th June 2020). 
  10. A Review on HPLC-Trouble Shooting Guide by Akshaykumar VB, Jitendra SS, Hitendra BP, et al. 2014; 27: 200–9. International Journal of Pharmaceutical Science and Research. 
  11. Review of High Performance Liquid Chromatography by Mukthi T. (HPLC). J Pharm Anal 2016; 5: 22-8 (Research & Reviews). 
  12. High Performance Liquid Chromatography in Analysis, by Divya TN. 2014; 3: 73-5 in Research & Reviews in Pharmacy and Pharmaceutical Sciences. 
  13.  Michael S. HPLC detectors: A quick overview. J. Liq. Chromatogr. 
  14. A Manual for Problem-Solving in High Performance Liquid Chromatography, Pankti PD. (2012) 5 J Pharm Res 4411-20. 
  15. HPLC Chromatography: A Working Methodology and Its Principle. Hplc chromatography principle may be found at https://www.studyread.com/(Accessed on 7th July 2020). 
  16. Quantitative HPLC mistakes. qnt_err_hplc.ht http://www.interchromforum.com ml (Accessed on7th July 2020). 
  17. The need for HPLC mobile phase degassing. Why is degassing of the HPLC mobile phase necessary? https://lab-training.com/2013/11/18(Accessed on 7th July 2020). 
  18. Advice on how long the lines on my HPLC system should be purged. http://blog.mournetrainingservices.co.uk/2014/01/helpon-how-long-should-it-take-to.html (Accessed on 7th July 2020). 
  19. SOP for high performance liquid chromatography cleaning. The URL for this page is: https://www.pharmaguideline.com/2011/08/sop-for-cleaning-of-high-performance.html#gsc.tab=0 (Accessed on 7th July 2020). 
  20. Know what you are putting into your HPLC during the mobile phase. Know what you are putting into your HPLC during the mobile phase, according to https://www.lifecyclebio.com/2018/05/25/ (Accessed on 6th July 2020). 
  21. Ozlem C. Chromatography as a separation method, North Clin Istanbul 2016;3:156–60. 
  22. Chromatographers' Hints and Tips for HPLC Analysis. https://hplctips.blogspot.com/2014/01/diagnosingtroubleshooting-hplc.html (Accessed on 20th July 2020). 
  23. Michael ES. Introduction and Review of UPLC. 2005; 28: 1253-63 J Liq Chromatogr R T. 
  24. A Review on the Development and Validation of Analytical Methods in Pharmaceutical Technology. Review on Analytical Method Development and Validation of Pharmaceutical Technology, available at https://www.pharmatutor.org (Accessed on 6th July 2020). 
  25. Stavros Kromidas, number 25. Additional Practical HPLC Problem Solving, Wiley, 2004. 
  26. HPLC, available at https://lab-training.com/hplc (Accessed on 10th July 2020). 
  27. J Pharm Edu Res 2017;1:243–60. Patil MP. HPLC Method Development-A Review. 
  28. Degassing of the mobile phase. What is mobile phase degassing? may be found at https://www.chromatographytoday.com/news/hplc- uhplc/31/breaking-news/31347. (Accessed on 20th July 2020). 
  29. Analytical approaches in pharmaceutical analysis: A review, Siddiqui MR, AlOthman ZA, Rahman N. AJCh 2017;10:S1409–S1421. 
  30. Can Air Hurt My Column? Does air destroy my column? : liquid chromatography (https://blog.sepscience.com/) (Accessed on 20th July 2020). 
  31. A Review of Ultra Performance Liquid Chromatography by Anagha SP (UPLC). 2015; 3(10); 86–96 in Asian J Pharm Tech Innovation

Photo
Snehal Ugale
Corresponding author

Department of Pharmaceutical Quality Assurance, PRES College of Pharmacy (Women’s), Chincholi, Nashik-422102

Photo
Vinayak Gaware
Co-author

Department of Pharmaceutical Chemistry, PRES College of Pharmacy (Women’s), Chincholi, Nashik-422102

Photo
Rahul Khaire
Co-author

Department of Pharmaceutical Chemistry, PRES College of Pharmacy (Women’s), Chincholi, Nashik-422102

Photo
Charushila Bhangale
Co-author

Department of Pharmaceutical Chemistry, PRES College of Pharmacy (Women’s), Chincholi, Nashik-422102

Snehal Ugale*, Vinayak Gaware, Rahul Khaire, Charushila Bhangale, A Systematic Guide On HPLC Troubleshooting, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 4, 197-209. https://doi.org/10.5281/zenodo.7883558

More related articles
Protective Effect of Phoenix Dactylifera Seed Extr...
Sagar Sahu, Sandeep Jain, Urvesh Singh Narwaria...
Analysis of Environmental and Microbial Determinan...
Raja Waleed Sajjad, Dr. Jaweria Hashmi, Raja Ahmed Sajjad, Dr. Kh...
Pharmaceutical Gel: An Overview...
Raisey Jose, Fahana Shirin, Fathimathu Sana P, Nishitha K M, Soy...
View more
Preparation And Evaluation Of Polyherbal Topical Gel For Dysmenorrhea...
Namrata Patil, C.M.Jangme, Pooja Deshmane, Ruksar Mulla , Gayatri Patil, Samruddhi Patil...
Quality by Design (QbD) in Analytical Method Development for Dosage Forms: A Cri...
Dr. Rahul Solunke, Sumedh Sonkamble, Anusaya Babalsure, Vaishali Satpute, Amarpali pawar...
Role of Automation In Pharmaceutical Manufacturing Industry...
Tejashree Burungale , Dr. Rajendra Patil, Dr. Swati Burungale, Nikita Gavali, Pankaj Shinde ...
View more
Download PDF
Related Articles
Cold Cream Conundrum: A Comparative Evaluation Of Marketed Products For Optimal ...
Pallavi Bansod, N. D. Gaidhani, Prasnjeet Raibole...
Herbal Ph-Responsive Lipstick : Formulation, Evaluation, and Applications...
Rathod Devanshi, Dr. Dhaval Patel, Dr. Nishith Patel, Mishra Mansi, Kiran Sahoo...
A Comprehensive Review on Analytical Methods for the Determination of Cinitaprid...
Aditi Bangar, Prachi Kanawade...
Development and Validation of First-Order Derivative and Area Under Curve Method...
Santosh Karajgi, Adarsh Patil, Achhegav Rajkumar, Dharmanna Salutagi, Rakshit Hulamani, Danesh Takko...
Protective Effect of Phoenix Dactylifera Seed Extract against Scopolamine Induce...
Sagar Sahu, Sandeep Jain, Urvesh Singh Narwaria...
More related articles
Protective Effect of Phoenix Dactylifera Seed Extract against Scopolamine Induce...
Sagar Sahu, Sandeep Jain, Urvesh Singh Narwaria...
Analysis of Environmental and Microbial Determinants of Preterm Birth in Pakista...
Raja Waleed Sajjad, Dr. Jaweria Hashmi, Raja Ahmed Sajjad, Dr. Khalil Ahmed Hashmi, Moazzam Ali Mali...
Pharmaceutical Gel: An Overview...
Raisey Jose, Fahana Shirin, Fathimathu Sana P, Nishitha K M, Soya Benny...
View more
Protective Effect of Phoenix Dactylifera Seed Extract against Scopolamine Induce...
Sagar Sahu, Sandeep Jain, Urvesh Singh Narwaria...
Analysis of Environmental and Microbial Determinants of Preterm Birth in Pakista...
Raja Waleed Sajjad, Dr. Jaweria Hashmi, Raja Ahmed Sajjad, Dr. Khalil Ahmed Hashmi, Moazzam Ali Mali...
Pharmaceutical Gel: An Overview...
Raisey Jose, Fahana Shirin, Fathimathu Sana P, Nishitha K M, Soya Benny...
View more

Copyright © 2026 IJPS. All rights reserved