Background: Macrolides are bacteriostatic antibiotics used in the management of mild to chronic soft tissues, and upper and lower respiratory and genitourinary tract infections. Misuse of these agents is common among people with infections, thus leading to the development of bacterial resistance. Also, due to high demand, there likelihood of fake and substandard products in the markets, hence the need to develop simple, cost-effective, and rapid methods for the analysis and percentage determination of these antibiotics. The study aimed to quantify various samples of macrolide using thin-layer chromatographic and UV-spectrophotometric analytical methods. Silica gel GF 254 was used as the stationary phase for TLC, while in UV spectrophotometric analysis, samples were identified through absorbance produced at different wavelengths (200 – 350 nm). All the samples were within standard weight (mg), and the spectrophotometry fingerprinting analysis was obtained at a wavelength of 220 nm and 240 nm. The retardation factor (Rf) value calculated for the various macrolide ranged from 0.75 to 0.83 with visible spots on exposure to iodine vapor. The use of appropriate methods of detection and solvent systems permits the identification of the entire macrolide antibiotics samples used in the analysis. TLC and Spectrophotometric fingerprinting procedures can be applied in preparatory and exploratory analytical screening, quality control studies, and therapeutic drug monitoring of macrolide antibiotics.


macrolide, analytical method, chromatography, azithromycin, spectrophotometry


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Samuel J. Bunu
Corresponding author

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.

Veronica Aniako

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.

Varsharani P. Karade

Dadasaheb Balpande College of Pharmacy, Besa, Nagpur, Maharashtra, India.

Edebi N. Vaikosen

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.

Benjamin U. Ebeshi

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.

Samuel J. Bunu, Veronica Aniako, Varsharani P. Karade, Edebi N. Vaikosen, Benjamin U. Ebeshi, Thin-Layer Chromatographic And UV-Spectrophotometric Analysis Of Frequently Utilized Oral Macrolide Antibiotics, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 9, 265-274.

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