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  • Ameliorative effect of Dhatryadi Ghrita on Letrozole induced Polycystic Ovarian Syndrome in female albino Wistar rats

  • 1Department of Pharmacology, Student of Pharmacy, Madras Medical College, Chennai, India.
    2Department of Pharmacology, Faculty of Pharmacy, Madras Medical College, Chennai, India.

Abstract

Objective: To investigate ameliorative effects of Dhatryadi ghrita on letrozole induced PCOD in female albino Wistar rats. Methods: Letrozole was given orally to rats every day for 21 days at a dose of 1 mg/kg body weight to induce PCOS. PCOS rats were then treated daily with metformin (70mg/Kg) and DG (200mg/Kg and 400mg/Kg) for 15 d. Rats which underwent none of these treatments were kept as control. Serum glucose, Lipid profile, insulin, estrogen, progesterone levels and superoxide dismutase levels were determined. Results: Rats treated with letrozole demonstrated a significant increase in body weight, serum triglycerides, cholesterol, LDL, glucose and insulin, and a significant decline in progesterone, Oestrogen, HDL and SOD levels compared to control. Rats treated with metformin, DG 200mg/Kg and DG 400mg/Kg showed a remarkable decrease in the elevated body weight, blood glucose, LDL, TC, TG and insulin levels and significant increase in the serum oestrogen, progesterone, SOD and HDL levels. Conclusion: Data indicate that DG exert potential ameliorative effects against PCOS through the modulation of hormonal and lipid profile as well as oxidative stress. These beneficial impacts of Dhatryadi ghrita make it a promising agent for the treatment and prevention of PCOS. Furthermore, these substances' beneficial effects are on par with those of metformin.

Keywords

Dhatryadi ghrita (DG); Metformin; Polycystic Ovarian Syndrome; hormone analysis; oxidative stress; hyperinsulinemia

Reference

  1. Samer El Hayek, Lynn H. Hamdar, Fadi G. Mirza and Georges Daoud.Polycystic Ovarian Syndome: An Updated Overview.Front.Physiol.7: 124. https://doi.org/10.3389/fphys.2016.0012. 
  2. Azziz, R., Woods, K. S., Reyna, R., Key, T. J., Knochenhauer, E. S., and Yildiz, B. O. (2004). The prevalence and features of the polycystic ovary syndrome in an unselected population. J. Clin. Endocrinol. Metab. 89, 2745–2749. https://doi.org/10.1210/jc.2003-032046. 
  3. March WA, Moore VM, Willson KJ, Phillips DI, Norman RJ, Davies MJ: The prevalence of polycystic ovary syndrome in a community sample assessed under contrasting diagnostic criteria. Hum Reprod 2010, 25:544-551. https://doi.org/10.1093/humrep/dep399. 
  4. H Teede, A Deeks and L Moran. Polycystic ovary syndrome: a complex condition with psychological, reproductive and metabolic manifestations that impacts on health across the lifespan. BMC Medicine2010,8:41. http://www.biomedcentral.com/1741-7015/8/41.
  5. Roe AH, Prochaska E, Smith M, Sammel M, Dokras A. Using the Androgen Excess-PCOS Society criteria to diagnose polycystic ovary syndrome and the risk of metabolic syndrome in adolescents. J Pediatr 2013;162:937–941. https://doi.org/10.1016/j.jpeds.2012.11.019. 
  6. Badawy A, Elnashar A. Treatment options for polycystic ovary syndrome. Int J Women’s Health 2011;3:25–35. https://doi.org/10.2147/IJWH.S11304.
  7. Fauser BCJM, Tarlatzis BC, Rebar RW, et al. Consensus on women’s health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group. Fertil Steril 2012;97:28–38. https://doi.org/10.1016/j.fertnstert.2011.09.024. 
  8. Balen AH, Conway GS, Kaltsas G, Techatrasak K, Manning PJ, West C, Jacobs HS: Polycystic ovary syndrome: the spectrum of the disorder in 1741 patients. Hum Reprod 1995, 10:2107-2111. https://doi.org/10.1093/oxfordjournals.humrep.a136243. 
  9. Moran LJ, Pasquali R, Teede HJ, Hoeger KM, Norman RJ: Treatment of obesity in polycystic ovary syndrome: a position statement of the Androgen Excess and Polycystic Ovary Syndrome Society. Fertil Steril 2009, 92:1966-1982. https://doi.org/10.1016/j.fertnstert.2008.09.018. 
  10. R. J. Norman, M. J. Davies, J. Lord, and L. J. Moran, “The role of lifestyle modification in polycystic ovary syndrome,” Trends in Endocrinology and Metabolism, vol. 13, 2002. https://doi.org/10.1016/s1043-2760(02)00612-4. 
  11. Carrie C. Dennett and Judy Simon. The Role of Polycyctic Ovary Syndrome in Reproductive and Metabolic Health: Overview an Approaches for Treatment. https://doi.org/10.2337/diaspect.28.2.116. 
  12. C. M. J. Matyanga and B. Dzingirai, “Clinical pharmacology of hormonal emergency contraceptive pills,” International Journal of Reproductive Medicine, vol. 2018. https://doi.org/10.1155/2018/2785839. 
  13. Verit FF, Erel O. Oxidative stress in nonobese women with polycystic ovary syndrome: correlations with endocrine and screening parameters. Gynecol Obstet Investig. 2008;65(4):233–9. https://doi.org/10.1159/000113046.
  14. Sies H. Oxidative stress: from basic research to clinical application. Am J Med. 1991;91(3):S31–8. https://doi.org/10.1016/0002-9343(91)90281-2.
  15. Goodarzi.M.O.etal. Polycycstic ovary syndrome: etiology, pathogenesis and diagnosis.NatRev Endocrinol 2011;7:219–2311. https://doi.org/nrendo2010.217.Epub2011. 
  16. Rashmi Saxena Pal, Amrita Mishra.A review on Dhatryadi ghrita.ijrap. https://doi.org/10.7897/2277-4343.082111.
  17. OECD Acute Oral toxicity 423.,2001,[01]. https;//ntp.nieghs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf. 
  18. Amal H. Hamza, Widad M. AlBishri, Mona H. Alfaris.Effect of Vitex-castus plant extract on polycystic ovary syndrome complications in experimental rat model. apjr.2019;8(2): 63-69. http://doi.org/10.4103/2305-0500.254647. 
  19. Anam Younas, Liaqat Hussain, Arham Shabbir, Muhammad Asif, Musaddique Hussain, and Faiza Manzoor.Effects of Fagonia indica on letrozole-induced polycystic ovarian syndrome (PCOS) in young adult female rats. Hindawi.2022.1397060.13. https://doi.org/10.1155/2022/1397060.
  20. Yasmine F. Ibrahim, Mohammed Alorabi, Walaa Yehia Abdelzaher, Nisreen DM Toni, Khaled Thabet, AbdelRahman Hegazy, Haitham Ahmed Bahaa, Gaber El-Saber Batiha, Nermeen N. Welson, Mohamed A. Myrosy, Katharigatta N. Venugopala, Asmaa Mohamed Abdel-Aziz.Diacerein ameliorates letrozole-induced polycystic ovarian syndromeinrats.Biomedicine&Pharmacotherapy.149(2022)112870.  https://doi.org/10.1016/j.biopha.2022.112870.

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Sangeetha Venkatesan
Corresponding author

Department of Pharmacology, Student of Pharmacy, Madras Medical College, Chennai, India.

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Sakthi Abirami
Co-author

Department of Pharmacology, Faculty of Pharmacy, Madras Medical College, Chennai, India.

Sangeetha Venkatesan, Sakthi Abirami, Ameliorative effect of Dhatryadi Ghrita on Letrozole induced Polycystic Ovarian Syndrome in female albino Wistar rats, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 9, 417-426. https://doi.org/10.5281/zenodo.8378350

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