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  • Ameliorative effect of omega 3 Fatty acid on Cypermethrin induced developmental neurotoxicity in chick embryo
  • Institute of Pharmaceutical Sciences, Kurukshetra University Kurukshetra, Haryana-136119.

Abstract

Pyrethroids are a class of insecticides that are used for crop protection worldwide. Cypermethrin, a class II pyrethroid, is used to control insects in the household and agricultural fields. It crosses the blood-brain barrier and induces neurotoxicity and motor deficits. Cypermethrin prolongs the opening of sodium channel, thereby causing depolarisation leading to hyper-excitation of the central nervous system. It also modulates chloride, voltage-gated calcium and potassium channels, alters the activity of glutamate and acetylcholine receptors and adenosine tri-phosphatases and induces DNA damage and oxidative stress in the neuronal cells. The present study aimed to evaluate the Cypermethrin induced developmental neurotoxicity in chick embryo. We have also investigated the ameliorative effect of omega-3-Fatty acid on Cypermethrin induced toxicity. Sixty eggs (0 day) of species Gallus domesticus were used and divided into six groups -control, vehicle, CYP (50 ppm), CYP (100ppm), CYP (50ppm) + DHA (200µg/egg) and CYP (100ppm) + DHA (200µg/egg) were kept in an incubator at 38±0.5°C with a relative humidity of 60-70% and proper ventilation. Control group have untreated eggs. The vehicle group was supplied with distilled water while the eggs in remaining four groups were administered by air sac method. 16-day old embryos were recovered from all the groups for assessing morphometric, biochemical parameters and histopathology. Results showed that group treated with higher dose of cypermethrin (100ppm) showed a marked decrease in morphometric parameters. Administration of docosahexanoic acid (200µg) in combination with cypermethrin ameliorated the neurological changes produced by Cypermethrin alone in the chick embryo.

Keywords

Cypermethrin (CYP), Omega-3-fatty acid, docosahexanoic acid (DHA), Gallus domesticus, morphometric parameters L.

Reference

  1. Abdou HM, Hussien HM and Yousef MI (2012). Deleterious effects of cypermethrin on rat liver and kidney: Protective role of sesame oil. Journal of Environmental Science and Health. Part B. 47(4): 306-314.
  2. Aebi H (1984). Catalase in vitro. In Methods in enzymology. Academic Press. 105:121-126.
  3. Alaa-Eldin EA, El-Shafei DA and Abouhashem NS (2017). Individual and combined effect of chlorpyrifos and cypermethrin on reproductive system of adult male albino rats. Environmental Science and Pollution Research. 24(2):1532-1543.
  4. Anwar K (2003). Cypermethrin, a pyrethroid insecticide induces teratological and biochemical changes in young chick embryos. Pakistan Journal of Biological Sciences. 6(19):1698-1705.
  5. Anwar K (2003). Toxic Effects of Cypermethrin on the Biochemistry and Morphology of 11th Day Chick Embryo (Gallus domesticus). Pakistan Journal of Applied Sciences. 3(6):432-445.
  6. Anwar K, and Shakoori AR (2010). Cypermethrin toxicity in the liver of developing chick embryo. Pakistan Journal of Zoology. 42(6):725-733.
  7. Arteaga O, Revuelta M, Urigüen L, Martínez-Millán L, Hilario E and Álvarez A (2017). Docosahexaenoic acid reduces cerebral damage and ameliorates long-term cognitive impairments caused by neonatal hypoxia–ischemia in rats. Molecular Neurobiology. 54(9):7137-7155.
  8. Assayed ME, Khalaf AA and Salem HA (2010). Protective effects of garlic extract and vitamin C against in vivo cypermethrin-induced teratogenic effects in rat offspring. Food and Chemical toxicology. 48(11):3153-3158.
  9. Benzie IF and Strain JJ (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. In methods in enzymology. Academic Press. 299:15-27. 
  10. Berman DR, Mozurkewich E, Liu Y, Shangguan Y, Barks JD and Silverstein FS (2013). Docosahexaenoic acid augments hypothermic neuroprotection in a neonatal rat asphyxia model. Neonatology. 104(1):71-78.
  11. Ellman GL, Courtney KD, Andres JrV and Featherstone RM (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology. 7(2):88-95.
  12. Greene S and Pohanish R. Sittig’s handbook of pesticides and agricultural chemicals. 1st edition. Lawyers cooperative publishing company. William Andrew imprint. Norwich, New York. 2005. pp. 174–177.
  13. Griffin SP and Bhagooli R (2004). Measuring antioxidant potential in corals using the FRAP assay. Journal of Experimental Marine Biology and Ecology. 302(2):201-211.
  14. Hocine L, Merzouk H, Merzouk SA, Ghorzi H, Youbi M, and Narce M (2016). The effects of alpha-cypermethrin exposure on biochemical and redox parameters in pregnant rats and their newborns. Pesticide Biochemistry and Physiology. 134:49-54.
  15. Kishore RK, Sudhakar D and Parthasarathy PR (2009). Embryo protective effect of pomegranate (Punica granatum L.) Fruit extract in adriamycin-induced oxidative stress. Indian Journal of Biochemistry and Biophysics. 46:106-111.
  16. Landrigan PJ, Kimmel CA, Correa A, and Eskenazi B (2004). Children's health and the environment: public health issues and challenges for risk assessment. Environmental Health Perspectives. 112(2): 257-265.
  17. Levi PE. Toxic action: A Textbook of modern toxicology. 1st edition. Edley publications. New York. 1987:172-174.
  18. Marklund S and Marklund G (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. The FEBS Journal. 47(3):469-474.
  19. Natekar PE (2007). Methotrexate induced gross malformations in chick embryos. Journal of Human Ecology. 21(3):223-226.
  20. Ohkawa H, Ohishi N and Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 95(2):351-358.
  21. Paskova V, Hilscherova K and Bláha L (2011). Teratogenicity and embryotoxicity in aquatic organisms after pesticide exposure and the role of oxidative stress. In Reviews of Environmental Contamination and Toxicology. 211:25-61.
  22. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG and Hoekstra W (1973). Selenium: biochemical role as a component of glutathione peroxidase. Science. 179(4073):588-590.
  23. Series FPD (2000). Baseline study on the problem of obsolete pesticide stocks Rome Italy. Food and Agriculture Organization of Pesticide Disposal Series. 1(1):01-20.
  24. Uggini GK, Patel PV, and Balakrishnan S (2012). Embryotoxic and teratogenic effects of pesticides in chick embryos: a comparative study using two commercial formulations. Environmental Toxicology. 27(3):166-174.
  25. Van Gelder MM, Van Rooij IA, Miller RK, Zielhuis GA, de Jong-van den Berg LT and Roeleveld N (2010). Teratogenic mechanisms of medical drugs. Human Reproduction Update. 16(4):378-394.
  26. World Health Organization (WHO) (2002). Recommended classification of pesticide by hazard, and guidelines to classification Geneva. WHO International Programme on Chemical Safety Publications. 1(1):01-25.

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Rinku
Corresponding author

Institute of Pharmaceutical Sciences, Kurukshetra University Kurukshetra, Haryana-136119.

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Shivkant Sharma
Co-author

Institute of Pharmaceutical Sciences, Kurukshetra University Kurukshetra, Haryana-136119.

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Dhirender Kaushik
Co-author

Institute of Pharmaceutical Sciences, Kurukshetra University Kurukshetra, Haryana-136119.

Rinku*, Shivkant Sharma, Dhirender Kaushik, Ameliorative effect of omega-3-Fatty acid on Cypermethrin induced developmental neurotoxicity in chick embryo, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 9, 197-208. https://doi.org/10.5281/zenodo.8331551

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