Institute of Pharmaceutical Sciences, Kurukshetra University Kurukshetra, Haryana-136119.
Pyrethroids are a class of insecticides that are used for crop protection worldwide. Cypermethrin, a class II pyrethroid, is used to control insects in the household and agricultural fields. It crosses the blood-brain barrier and induces neurotoxicity and motor deficits. Cypermethrin prolongs the opening of sodium channel, thereby causing depolarisation leading to hyper-excitation of the central nervous system. It also modulates chloride, voltage-gated calcium and potassium channels, alters the activity of glutamate and acetylcholine receptors and adenosine tri-phosphatases and induces DNA damage and oxidative stress in the neuronal cells. The present study aimed to evaluate the Cypermethrin induced developmental neurotoxicity in chick embryo. We have also investigated the ameliorative effect of omega-3-Fatty acid on Cypermethrin induced toxicity. Sixty eggs (0 day) of species Gallus domesticus were used and divided into six groups -control, vehicle, CYP (50 ppm), CYP (100ppm), CYP (50ppm) + DHA (200µg/egg) and CYP (100ppm) + DHA (200µg/egg) were kept in an incubator at 38±0.5°C with a relative humidity of 60-70% and proper ventilation. Control group have untreated eggs. The vehicle group was supplied with distilled water while the eggs in remaining four groups were administered by air sac method. 16-day old embryos were recovered from all the groups for assessing morphometric, biochemical parameters and histopathology. Results showed that group treated with higher dose of cypermethrin (100ppm) showed a marked decrease in morphometric parameters. Administration of docosahexanoic acid (200µg) in combination with cypermethrin ameliorated the neurological changes produced by Cypermethrin alone in the chick embryo.
Rinku*, Shivkant Sharma, Dhirender Kaushik, Ameliorative effect of omega-3-Fatty acid on Cypermethrin induced developmental neurotoxicity in chick embryo, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 9, 197-208. https://doi.org/10.5281/zenodo.8331551