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  • Analytical Method Development and Validation for Lopinavir and Ritonavir in Bulk and Dosage Form
  • Department of Pharmaceutical Quality Assurance, Rajarshi Shahu College of Pharmacy, Buldana 443001, Maharashtra, India.

Abstract

Objective: A simple, accurate and precise RP-HPLC method for simultaneous determination of Lopinavir and Ritonavir in bulk and dosage forms. Methods: A reversed phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the quantitative determination of lopinavir (LPV) and ritonavir (RTV) on Agilent C18 (2) 250 × 4.6 mm, 5 ? column as a stationary phase and mobile phase composition of Acetonitrile: 0.1% Ortho-phosphoric acid (pH 3.5) (80:20, v/v) at a flow rate of 1ml/min. Results: Quantification was achieved with UV detection at 217 nm. The retention times of Lopinavir and Ritonavir was 7.38 and 2.98 min respectively. The result of linearity was obtained in the concentration range of 0-20µg/ml for both Ritonavir and Lopinavir. %Recovery was Obtained as 99.25% and 99.72% for Ritonavir and Lopinavir respectively. LOD, LOQ values are obtained from regression equations of Ritonavir and Lopinavir were 4.6ng/spot, 1.5ng/spot, 5.10ng/spot, 21.00ng/spot respectively. Regression equation of Ritonavir and Lopinavir Y= 0.1828x - 0.0236 and y = 0.1639x - 0.0261. Retention times are decreased and that run time was decreased so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Keywords

ritonavir, lopinavir, acetonitrile, RP-HPLC, validation

Reference

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Archana B. Chavhan
Corresponding author

Department of Pharmaceutical Quality Assurance, Rajarshi Shahu College of Pharmacy, Buldana 443001, Maharashtra, India.

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Vijay S. Borkar
Co-author

Department of Pharmaceutical Quality Assurance, Rajarshi Shahu College of Pharmacy, Buldana 443001, Maharashtra, India.

Archana B. Chavhan*, Vijay S. Borkar, Analytical Method Development and Validation for Lopinavir and Ritonavir in Bulk and Dosage Form, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 8, 111-117. https://doi.org/10.5281/zenodo.8247500

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