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Abstract

Aim: Creating and assessing an herbal hair mask. Materials and method: Herbs having antibacterial, anti-inflammatory, and antioxidant qualities are used to make herbal hair products. Several plant parts were selected to make the herbal hair mask. Wheatgrass powder, aloe vera powder, hibiscus powder, amla powder, multani powder, and Heena powder were the natural ingredients used to create the hair mask. The components must be collected, cleaned, dried, powdered, weighed, and mixed. When assessing the herbal hair mask formulation, several parameters were determined, and the results are reported in this study. These factors include pH, washability (what kind of hair after washings), eye irritation, patch test, and stability test also performed in this the color odor and texture smoothness were evaluated, as physical, chemical, organoleptic evaluation, and so on. Results: After the criteria (pH, washability, post-wash hair type, and ocular irritation) were evaluated, a patch test was performed to create an herbal hair mask. The results indicated that the parameters fell within an acceptable range. Conclusion: Based on this investigation, it has been determined that the herbal hair mask formulation is safer, more effective, and more reasonably priced. It also contains all the desirable characteristics of the ideal hair mask. The hair mask that was made has no chemicals at all. Because it only contains natural ingredients, it doesn't harm hair and gives it a smooth texture. It also helps in hair growth and avoids hair-related problems.

Keywords

herbal, hair mask, chemicals, wheat grass

Introduction

Hair masks are a great way to shield your hair from the environment, pollution, and the sun.  The intricate and ever-changing system that governs normal hair formation is still a mystery.  This cyclical cycle includes the synthesis, elongation, and eventual loss of hair shafts.  Follicles in the antigen, cartage, and telogen phases make up human hair.  Our hair follicle actively gathers and rapidly grows cytochrome during the antigen phase.  Our body's cells should be prepared to transition into new development cycles at the telogen stage (when we shed them). Still, they hibernate because they are not receiving enough nourishment from their surroundings, exacerbating the dryness. For this reason, using moisture-rich hair masks regularly is crucial to maintaining the health of your hair without compromising its appearance.  If you have an oily or dry scalp, hair masks are a terrific way to hydrate your hair.  They can also encourage healthy development and lessen hair loss.  You probably wouldn't mind using a hair mask daily if you love your hair.  Retaining moisture can help keep your hair hydrated while also reviving its growth and strength. Hair loss, damage, and dry scalp can all be effectively treated with natural hair masks.  Hair masks can strengthen our hair and aid in improving the state of our scalp.  You will see benefits immediately after using these masks daily.  We developed this product to assist consumers in improving the health of their scalp and hair.  Our hair mask is chemical-free, simple to make, and leaves no residue on your head!  For all hair types, our hair mask is a healthy and stimulating treatment.  It strengthens our hair and feeds the scalp, making it healthier and more attractive. We make this mask with natural ingredients.

MATERIALS:

Herbal Ingredients:

The different parts of the plants were selected for study having hair care properties which is already proven. Following are the ingredients that we have to use in the formulation of the hair mask.

Wheatgrass powder 

Neem Powder.

Hibiscus Powder.

Amla powder.

Henna powder 

Aloe powder

Muitani powder

1. Wheat grass & Wheatgrass powder:

    

Fig.1: Wheatgrass &powder

Pharmacognosy study of wheatgrass (Triticum aestivum):

Botanical Source

Scientific Name: Triticum aestivum Linn.

Family: Poaceae (Gramineae)

Common Name: Wheatgrass 

Part Used: Fresh grass (7-10 days old), juice, and powder.

Chemical Constituents:

Chlorophyll

Vitamins: A, C, E, K, B-complex

Minerals: Calcium,     Iron,    Magnesium, Potassium

Enzymes: Superoxide dismutase (SOD), cytochrome oxidase

Amino acids

Flavonoids and phenolic compounds

Therapeutic Uses:

Anemia

Thalassemia (as an adjunct therapy)

Cancer support (complimentary)

Diabetes management

Detoxification Skin disorders

Constipation and digestive problems

2.  Neem:

     

Fig.2: Neem & Neem Leaf Powder

Pharmacognosy Study of Neem (Azadirachta indica)

Botanical Source: 

Scientific Name: Azadirachta indica A. Juss. Family: Meliaceae

Common Name: Neem, Margosa tree, Indian lilac

Parts Used: Leaves, bark, seeds, oil, flowers

Chemical Constituents:

Limonoids: Azadirachtin (main insecticidal component)

Flavonoids: Quercetin

Triterpenoids: Nimbin, nimbidin, nimbolide

Therapeutic Uses:

Skin diseases (eczema, acne, wounds)

Dental care (toothpaste, mouthwash)

Malaria, fever

Diabetes support

Digestive disorders  (ulcers, worm infestations)

Liver protection

Insect bites and repellents

3. Hibiscus:

    

Fig.3: Hibiscus Flowers & powder

Pharmacognosy Study of Hibiscus (Hibiscus rosa Sinensis):

Botanical Source:

Scientific Name: Hibiscus rosa-sinensis Linn. Family: Malvaceae

Common Name: China Rose, Gurhal, Jaswand

Parts Used: Flowers, leaves, roots

Chemical Constituents:

Flavonoids (quercetin, cyanidin)

Anthocyanins

Mucilage

Tannins

Vitamin C

Alkaloids

Organic acids (citric, malic)

Therapeutic Uses:

Hypertension

Fever and cough

Menstrual disorders

Hair loss and dandruff

Skin infections

4. Amla:

  

Fig> 4: Amla& Amla powder

Pharmacognosy Study of Amla (Phyllanthus emblica)

Botanical Source:

Scientific Name: Phyllanthus emblica Linn.

Synonym: Emblica officinalis

Family: Phyllanthaceae (formerly Euphorbiaceae)

Common Name: Amla, Indian gooseberry

Chemical Constituents:

Vitamin C (Ascorbic acid): Highly stable form Tannins:      Emblicanin      A    &   B, punigluconin, pedunculagin

Phenolic compounds: Gallic acid, ellagic acid

Flavonoids: Quercetin, kaempferol

Amino acids, pectin, iron, calcium

Therapeutic Uses:

Indigestion, acidity

Diabetes and hyperlipidemia

Anemia and general debility

Cough, asthma, and respiratory conditions

Liver disorders

Hair fall and premature graying (used in oils)

Anti-aging and skin rejuvenation

5. Henna:

    

Fig.5: Henna Leaf & Henna powder

Pharmacognosy study of Henna (Lawsonia inermis)

Scientific name:-Lawsonia inermis Linn.

Family:- Lythraceae

Common Name: Henna, Mehandi, Egyptian privet

Parts Used: leaves, bark, flower, etc.

Chemical Constituents:

Naphthoquinones: Lawsone (2-hydroxy-1,4naphthoquinone)

Flavonoids

Tannins

Gallic acid

Mannitol

Essential oils

Resins and mucilage

Therapeutic Uses:

Skin infections, wounds, burns

Hair dye and conditioner

Dandruff treatment and scalp cooling

Mouth ulcers and gum diseases (leaf decoction as gargle)

Fever, headaches (paste applied topically)

Nail infections

Used traditionally for its cooling effect

6. Aloe Vera :

     

Fig.6: Aloe vera & Aloe powder

Pharmacognosy Study of Aloe (Aloe vera)

Botanical Source:

Scientific Name: Aloe vera (L.) Burm.f.

Synonym: Aloe barbadensis Mill.

Family: Asphodelaceae (formerly Liliaceae)

Common Name: Aloe, Ghritkumari Parts Used: Leaves – mainly the gel (mucilage) and latex (yellow sap)

Chemical Constituents:

Polysaccharides: Acemannan, glucomannan (found in the gel)

Anthraquinones: Aloin, aloe-emodin (in latex)

Enzymes: Catalase, peroxidase, amylase

Vitamins: A, C, E, B12

Minerals: Calcium, magnesium, zinc, selenium

Sterols: Campesterol, β-sitosterol

Saponins and lignin

Therapeutic Uses:

Burns, cuts, wounds, and sunburn

Skin diseases: acne, eczema, psoriasis

Constipation (latex, in small doses)

Oral ulcers, mouthwash for gingivitis

Cosmetics: Moisturizers, shampoos, lotions

Immunity booster and digestive aid (gel juice)

Diabetes (as adjuvant therapy)

7. Multani Mitti:

Fig.7: Multani powder

Pharmacognosy Study of Multani Mitti (Fuller’s

Earth)

Source & Origin:

Common Name: Multani Mitti, Fuller's Earth

Botanical Origin: It is naturally occurring clay, not from a plant but a mineral origin

Geological Source: Composed mainly of hydrous aluminum silicates

Traditional Source: Named after Multan (in Pakistan)

Chemical Composition:

Main Components:

Hydrated aluminum silicate

Magnesium, calcium, and iron oxides

Montmorillonite (a type of clay mineral)

Trace Elements: Silica, zinc, copper

Therapeutic and Cosmetic Uses:

Skincare: Face packs for acne, pimples, oily skin

Hair care: Removes excess oil and dandruff

Burns and inflammation: Applied as a cooling paste

Mud therapy: Used in naturopathy for skin diseases and detox

Wound healing: Acts as a protective and drying agent

METHODOLOGY: 

Composition: The largest dose of Wheat Grass and Hibiscus was taken since they have established hair nutrition properties, and because the scalp becomes dry during dandruff treatment, it is Critical to nourish it. Revitalize hair follicles, and promote hair growth. Because neem contains pungent active components in concentrated levels, it should only be used in small doses and Amla henna and aloe are used in lesser quantity.

Table 1: Composition of Herbal Hair Mask.

Sr.no

Name

Biological name

Quantity

1

Amla

Phyllanthus Embica

3g

2

Wheat Grass

Triticum

10g

3

Henna

Lawsonia inermis

3g

4

Aloe

barbadensis miller

3g

5

Hibiscus

Hibiscus rosa Sinensis

10g

6

Neem

Azadirachta indica

5g

7

Multani Mitti

Fuller's earth

q.s

         

Formulation:

The dried herbs were powdered and weighed precisely after being harvested and dried in the shade. In the mortar pestle, all of the components were homogeneously mixed, and passed through sieve no.80 as shown in the Herbal hair mask has been formulated using dried herbs that were precisely weighed and powdered after being harvested and dried in sunlight. "The components were then mixed homogeneously using a mortar and pestle. Ingredients are readily available. It is recommended that the mask be freshly prepared before usage. The herbal hair mask should be applied to the hair every week as a semi-solid paste in lukewarm water to improve the efficacy of the ingredients. The paste should have a consistent texture and be applied evenly to the hair with a brush, starting from the roots and working toward the tips. The hair mask should be allowed to dry completely for 30 minutes before rinsing it off with simple water.

Evaluation:

Organoleptic Evaluation: 

The examination of the formulation is performed under this evaluation, and it involves macroscopic aspects of the drug or product, such as color, scent, and taste, by using sensory organs such as the eyes or nose. Table 2 explains how macroscopic elements like texture and appearance are incorporated.

Table 2: Organoleptic evaluation.

Sr. No

Organoleptic Features

Observation

1

Color

Greenish Brown

2

Odor

Characteristics

3

Texture

Fine

4

Appearance

Coarse Powder

Physicochemical Research:

The formulation's physicochemical investigations were carried out as shown in Table 3 to examine physical and chemical characteristics such as pH, ash value, and loss on drying (Moisture content).

Table 3: Physicochemical Research.

Sr. No

Physiochemical Test

Observation

1

Ph

5.5

2

Moisture Content

91%

3

Ash Value

32%

Rheological Evaluation:

The physical properties of the powder formulation are examined in rheological examination, as indicated in Table 4. The flow ability of powers in this bulk density tapped density angle of repose Hauser’s ratio Carr's index is assessed using powder rheology.

Table 4: Rheological Evaluation.

1

Bulk Density

0.39

2

Tapped Density

0.51

3

Angle of repose

31.0

4

Car’s index

23.53%

5

Hausner’s Ratio

1.31

Patch test:  In this procedure, a small amount of moistened formulation is applied to the unit’s surface and the effect of the formulation irritancy and aching have or untitled

Table 5: Patch Test.

1

Swelling

Nil

2

Redness

Nil

3

Irritation

Nil

Stability test: The powdered formulation was kept at different temperatures (35 0Cand400C) and humidity levels for some time. The change in physical attributes was detected under various situations.

Table 6: Stability Test.

1

Change in colour

Nil

2

Change in odour

Nil

3

Change in PH

Nil

4

Change in texture

Nil

5

Change in smoothness

Nil

RESULT:

The herbal hair mask has been created to give the hair several advantages by utilizing the advantages of powerful phyto-constituents and natural herbs. Apart from giving the hair a moderate amount of nourishment, the mask also has anti-dandruff properties. The herbal hair mask's capacity to efficiently eliminate extra oil from the scalp is one of its main advantages.  Dandruff is frequently caused by an excess of oil on the scalp, which the herbal hair mask can assist with by eliminating the excess oil and lowering the chance of developing dandruff.   Overall, the herbal hair mask offers a natural and holistic approach to hair care by incorporating the benefits of natural herbs and phytoconstituents. By addressing multiple hair concerns, including dandruff and excess oil on the scalp, the mask can help to improve the overall health and appearance of the hair.

Procedure for hair mask application 

Take a required quantity of herbal hair mask in a bowl.

Apply the paste on the scalp to the ends by using a hairbrush or hand gloves.

Keep for 30 minutes then wash hair with diluted shampoo.

DISCUSSION

Benefits of Wheat Grass

1. Nourishment: Wheatgrass is rich in vitamins A, C, and E, iron, and calcium, which can nourish the hair and scalp.

2. Antioxidant properties: Wheatgrass antioxidants may help protect the hair from damage caused by free radicals.

3. Scalp health_: Wheatgrass may help soothe and calm the scalp, reducing irritation and inflammation.

Formulation Considerations

1. Stability: The stability of the wheatgrass extract in the hair mask formulation is crucial to ensure its effectiveness.

2. PH balance: The pH of the hair mask should be balanced to suit the scalp and hair.

Evaluation Parameters

1. Physical parameters: The hair mask's texture, consistency, and appearance were evaluated.

2. H and stability: The pH and stability of the formulation were assessed.

3. Hair growth promotion: The hair mask's potential to promote growth and improve hair health was evaluated.

Results and Implications

1. Improved hair health: The wheat grass hair mask showed potential benefits for hair health, including nourishment and antioxidant protection.

2. Scalp soothing: The formulation may help soothe and calm the scalp.

3. Future research: Further studies are needed to optimize the formulation and evaluate its long-term effects.

CONCLUSION:

In conclusion, a mild and gentle remedy for hair dandruff is the application of an herbal hair mask. Cosmetics made from herbs are renowned for being non-toxic and for nourishing the hair and scalp. With consistent usage, the herbal hair mask can help treat dandruff and leave hair healthy and problem-free by eliminating extra oil from the scalp. The positive effects of the herbal hair mask for dandruff have been demonstrated by this study; more research is required to identify any other advantages. Natural remedies are commonly utilized worldwide since they are thought to be safer and have fewer adverse effects.

REFERENCES

  1. Okereke JN, Udebuani AC, Ezeji EU, Obasi KO, Nnoli MC. Possible Health Implications Associated with Cosmetics A Review, Sci J Public Health 2015, 3(5-1): 58-63
  2. Mary P. Lupo. Antioxidants and Vitamins in Cosmetics ClimDermatol 2001; 19 467-473.
  3. Sowmya KV, Darsika CX, Grace F, Shanmuganathan S. Formulation & Evaluation of Poly-herbal Face wash gel. World J Pharm PharmSci 2015, 4(6), 585-588
  4. Millikan, Larry E. Cosmetology, Cosmetics, Cosmaceuticals Definitions and Regulations. ClinDermatol 2001; 19 (4), 371-374.
  5. Rieger MM.Harry's Cosmeticology, In: Chapter 23, Face, Body & Hair Masks & Scrubs 8th ed. vol L. New York: Chemical Publishing Co., Inc., 2009.p.471-483
  6. Zinnia. Ayurvedic Face Packs for Glowing Skin. Style Craze, Feb 2017 [cited 2017 Apr 24]. Available from: http://www.stylecraze.com/articles/5-ayurvedic-face-packs-for-glowing-skin
  7. Indian Standard, Face Pack-Specification, IS 15153: 2002, August 2002 [cited 2016 Aug 05]
  8. Hwang JK, Shim JS, Gwon SH, Kwon YY, Oh HI et al. Novel use of Panduratin derivatives or extract of Kaempferiapandurata comprising the same. The US. Patent 0065272A1,2012 [cited 2016 Aug 05]
  9. Michelle O'Sullivan, Turmeric is an effective homemade face pack ingredient to help open pores, Nov 2016 [cited 2016 Dec 13]. Available from: http://newswire.net/newsroom/pr/00094136.
  10. Best Benefits of Turmeric (Haldi) For Skin, Hair, And Health-No.4 Is the Best Nov 2016 [cited 2016 Dec 13]. Availablefrom.http//www.stylecraze.com/articles/turmeric-history-how-to-use-benefits.
  11. Rajeswari R. Umadevi M, Rahale CS, Pushpa R, Selvavenkadesh S, Sampath Kumar KP, Bhowmik D. Aloe vera. The Miracle Plant Its Medicinal and Traditional Uses in India. J PharmacognPhytochem 2012; 1(4): 118-124
  12. Nemade CT, Baste N. Formulation and evaluation of a herbal facial scrub World J Pharm Res 2014; 3(3): 4367-4371
  13. Himaja N, Ashok Kumar A, Bhartkumar B. Preparation and Evaluation of Poly Herbal Fruit Face Mask. J Res Pharm Sci 2015; 2(11): 07-13.
  14. Koli DS, Mane AN, Kumbhar VM, Shaha KS. Formulation & Evaluation of Herbal Anti-Acne Face Wash. World J Pharm PharmSci 2016, 5(6) 2001-2007.
  15. Yamini K. Onesimus T. Preparation and Evaluation of Herbal Anti-Acne Gel. Int J Pharm Bio Sci 2013; 4(2): 956
  16. Farheen B, Mohammad 1. Design and Development of Unani Face Pack for Skincare. European J Pharm Med Res 2016; 3(12): 627-632
  17. Buhse L., Kolinski R, Westenberger B, Wokovish A, Spencer J, Chen CW, et al. Topical Drug Classification. IntJ Pharm 2005, 295. 101-112.
  18. Banchhor M, Ashawat MS, Saraf S.Saraf S. Herbal Cosmetics: Trends in Skin Care Formulation. Pharmacogn Reviews 2009, 3(5): 82-89.
  19. Mandeep S, Shalini S, Sukhbir LK, Ram KS, Rajendra J. Preparation and Evaluation of Herbal Cosmetic Cream. Pharmacologyonline 2011; 1258-1264
  20. Rani SR and Hiremanth, Textbook of Industrial Pharmacy, Drug delivery systems& Cosmetics& Herbal drug technology: Universities Press (India) Ltd, 2nd Edition 2002
  21. Naresh G, Swetha P, Shilpa G. Formulation and Evaluation of Face Pack Containing Oats and Other Natural Ingredients. Int J Chem Pharm Sci 2013, 1(1): 28-34
  22. Andersen PH, Jensen NJ. Mutagenic investigation of peppermint od in the Salmonella/mammalian- microsome test. Mutat Res. 1984, 138:17-20. [PubMed] [Cross Ref]
  23. Anderson IB, Mullen WH, Meeker JE, et al. Pennyroyal toxicity measurement of toxic metabolite levels in two cases and review of the literature. Ann InternMed.1996,124:726-734.[PubMed] [Cross Ref]
  24. Bakerink JA, Gospe SM Jr, Dimand RJ, Eldridge MW. Multiple organ failure after ingestion of pennyroyal oil from herbal tea in two infants Pediatrics. 1996,98.944-947 [PubMed]
  25. Barceloux DG (2008).Pennyroyal and Pulegone (Mentha pulegium L.). In: Medical Toxicology of Natural Substances: Foods, Fungi, Medicinal Herbs, Plants and Venomous Animals. Hoboken, NJ: John Wiley & Sons, Inc., pp. 563-567.
  26. Cappello G, Spezzaferro M, Grossi L, Manzoli L, Marzio L. Peppermint oil (Mintoil) in the treatment of irritable bowel syndrome: a prospective double-blind placebo-controlled randomized trial. Dig Liver Dis. 2007; 39(6):530-6. [PubMed]
  27. Soosamma M, Chandrani D, Lubaina SZ A comparison of plant extracts vs. Chemical compounds as Antifungal agents against Malassezia spp. Int J Pharma Bio Sci, vol. 3, no. 3, 2012, pp. 773-80. M. Wuthi-udomlert, P.Chotipatoomwan, S.Panyadee, and W.Gritsanapan. Wuthi-udomlert, P. Chotipatoomwan, S. Panyadee, and W. Gritsanapan Malassezia furfur, yeast linked to dandruff, is Inhibited by designed lemongrass shampoo. S East Asian J Trop Med Public Health, vol. 42, no. 3, pp. 363-9, 2011,
  28. Michael A., Naveen S., Karthika S., Sentila R., Mahenthiran R. The effectiveness of herbal and chemical agents in the treatment of dandruff in vitro. J Microbiol Biotech Res, vol.2, no.2, 2012, pp. 916-21.
  29. -T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T
  30. P. Arora, A. Nanda, and M. Karan.A look into shampoos made with synthetic chemicals. In Comparison to shampoos containing natural components Int J Pharm Sci Rev is an international journal that publishes research on pharmaceutical science.
  31. [Flavonoids: hazards or therapeutic opportunities?]Firenzuoli F, Gori L, Crupi A, Neri D. Recenti Prog Med, vol. 95, ??. 8, pp. 345-351, 2004.
  32. Vanderwyk RW, Roia FC The human scalp's resident microbial flora and its association to dandruff 113-34 in J Soc Cosmet Chem, 1967. S Kumar, Akhila Akhila Akhila Akhila Akhila Akhila AkhilaAkhila Akhila Akhila Akhila Akhila Akhila Akhila In skincare, medicinal plants are used. C
  33. Hair, and Hair Diseases, Spring-variant Berlin Heidelberg 1990; pp.19-44.Orfanos CE, Happle R. Hair, and Hair Diseases, Spring-variant Berlin Heidelberg 1990; pp. 19-44.
  34. AD Vaidya, TP Devasagayam, T Devasagayam A summary of the current state of herbal medications In India. 1-11 in J Clin Biochem Nutr, 2007.

Reference

  1. Okereke JN, Udebuani AC, Ezeji EU, Obasi KO, Nnoli MC. Possible Health Implications Associated with Cosmetics A Review, Sci J Public Health 2015, 3(5-1): 58-63
  2. Mary P. Lupo. Antioxidants and Vitamins in Cosmetics ClimDermatol 2001; 19 467-473.
  3. Sowmya KV, Darsika CX, Grace F, Shanmuganathan S. Formulation & Evaluation of Poly-herbal Face wash gel. World J Pharm PharmSci 2015, 4(6), 585-588
  4. Millikan, Larry E. Cosmetology, Cosmetics, Cosmaceuticals Definitions and Regulations. ClinDermatol 2001; 19 (4), 371-374.
  5. Rieger MM.Harry's Cosmeticology, In: Chapter 23, Face, Body & Hair Masks & Scrubs 8th ed. vol L. New York: Chemical Publishing Co., Inc., 2009.p.471-483
  6. Zinnia. Ayurvedic Face Packs for Glowing Skin. Style Craze, Feb 2017 [cited 2017 Apr 24]. Available from: http://www.stylecraze.com/articles/5-ayurvedic-face-packs-for-glowing-skin
  7. Indian Standard, Face Pack-Specification, IS 15153: 2002, August 2002 [cited 2016 Aug 05]
  8. Hwang JK, Shim JS, Gwon SH, Kwon YY, Oh HI et al. Novel use of Panduratin derivatives or extract of Kaempferiapandurata comprising the same. The US. Patent 0065272A1,2012 [cited 2016 Aug 05]
  9. Michelle O'Sullivan, Turmeric is an effective homemade face pack ingredient to help open pores, Nov 2016 [cited 2016 Dec 13]. Available from: http://newswire.net/newsroom/pr/00094136.
  10. Best Benefits of Turmeric (Haldi) For Skin, Hair, And Health-No.4 Is the Best Nov 2016 [cited 2016 Dec 13]. Availablefrom.http//www.stylecraze.com/articles/turmeric-history-how-to-use-benefits.
  11. Rajeswari R. Umadevi M, Rahale CS, Pushpa R, Selvavenkadesh S, Sampath Kumar KP, Bhowmik D. Aloe vera. The Miracle Plant Its Medicinal and Traditional Uses in India. J PharmacognPhytochem 2012; 1(4): 118-124
  12. Nemade CT, Baste N. Formulation and evaluation of a herbal facial scrub World J Pharm Res 2014; 3(3): 4367-4371
  13. Himaja N, Ashok Kumar A, Bhartkumar B. Preparation and Evaluation of Poly Herbal Fruit Face Mask. J Res Pharm Sci 2015; 2(11): 07-13.
  14. Koli DS, Mane AN, Kumbhar VM, Shaha KS. Formulation & Evaluation of Herbal Anti-Acne Face Wash. World J Pharm PharmSci 2016, 5(6) 2001-2007.
  15. Yamini K. Onesimus T. Preparation and Evaluation of Herbal Anti-Acne Gel. Int J Pharm Bio Sci 2013; 4(2): 956
  16. Farheen B, Mohammad 1. Design and Development of Unani Face Pack for Skincare. European J Pharm Med Res 2016; 3(12): 627-632
  17. Buhse L., Kolinski R, Westenberger B, Wokovish A, Spencer J, Chen CW, et al. Topical Drug Classification. IntJ Pharm 2005, 295. 101-112.
  18. Banchhor M, Ashawat MS, Saraf S.Saraf S. Herbal Cosmetics: Trends in Skin Care Formulation. Pharmacogn Reviews 2009, 3(5): 82-89.
  19. Mandeep S, Shalini S, Sukhbir LK, Ram KS, Rajendra J. Preparation and Evaluation of Herbal Cosmetic Cream. Pharmacologyonline 2011; 1258-1264
  20. Rani SR and Hiremanth, Textbook of Industrial Pharmacy, Drug delivery systems& Cosmetics& Herbal drug technology: Universities Press (India) Ltd, 2nd Edition 2002
  21. Naresh G, Swetha P, Shilpa G. Formulation and Evaluation of Face Pack Containing Oats and Other Natural Ingredients. Int J Chem Pharm Sci 2013, 1(1): 28-34
  22. Andersen PH, Jensen NJ. Mutagenic investigation of peppermint od in the Salmonella/mammalian- microsome test. Mutat Res. 1984, 138:17-20. [PubMed] [Cross Ref]
  23. Anderson IB, Mullen WH, Meeker JE, et al. Pennyroyal toxicity measurement of toxic metabolite levels in two cases and review of the literature. Ann InternMed.1996,124:726-734.[PubMed] [Cross Ref]
  24. Bakerink JA, Gospe SM Jr, Dimand RJ, Eldridge MW. Multiple organ failure after ingestion of pennyroyal oil from herbal tea in two infants Pediatrics. 1996,98.944-947 [PubMed]
  25. Barceloux DG (2008).Pennyroyal and Pulegone (Mentha pulegium L.). In: Medical Toxicology of Natural Substances: Foods, Fungi, Medicinal Herbs, Plants and Venomous Animals. Hoboken, NJ: John Wiley & Sons, Inc., pp. 563-567.
  26. Cappello G, Spezzaferro M, Grossi L, Manzoli L, Marzio L. Peppermint oil (Mintoil) in the treatment of irritable bowel syndrome: a prospective double-blind placebo-controlled randomized trial. Dig Liver Dis. 2007; 39(6):530-6. [PubMed]
  27. Soosamma M, Chandrani D, Lubaina SZ A comparison of plant extracts vs. Chemical compounds as Antifungal agents against Malassezia spp. Int J Pharma Bio Sci, vol. 3, no. 3, 2012, pp. 773-80. M. Wuthi-udomlert, P.Chotipatoomwan, S.Panyadee, and W.Gritsanapan. Wuthi-udomlert, P. Chotipatoomwan, S. Panyadee, and W. Gritsanapan Malassezia furfur, yeast linked to dandruff, is Inhibited by designed lemongrass shampoo. S East Asian J Trop Med Public Health, vol. 42, no. 3, pp. 363-9, 2011,
  28. Michael A., Naveen S., Karthika S., Sentila R., Mahenthiran R. The effectiveness of herbal and chemical agents in the treatment of dandruff in vitro. J Microbiol Biotech Res, vol.2, no.2, 2012, pp. 916-21.
  29. -T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T. Vimaladevi, T
  30. P. Arora, A. Nanda, and M. Karan.A look into shampoos made with synthetic chemicals. In Comparison to shampoos containing natural components Int J Pharm Sci Rev is an international journal that publishes research on pharmaceutical science.
  31. [Flavonoids: hazards or therapeutic opportunities?]Firenzuoli F, Gori L, Crupi A, Neri D. Recenti Prog Med, vol. 95, ??. 8, pp. 345-351, 2004.
  32. Vanderwyk RW, Roia FC The human scalp's resident microbial flora and its association to dandruff 113-34 in J Soc Cosmet Chem, 1967. S Kumar, Akhila Akhila Akhila Akhila Akhila Akhila AkhilaAkhila Akhila Akhila Akhila Akhila Akhila Akhila In skincare, medicinal plants are used. C
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Nalawade Dipak
Corresponding author

Dr. Kolpe Institute of Pharmacy Kolpewadi, kopargaon 423601

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Dhule Poonam
Co-author

Dr. Kolpe Institute of Pharmacy Kolpewadi, kopargaon 423601

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Dhone Suvana
Co-author

Dr. Kolpe Institute of Pharmacy Kolpewadi, kopargaon 423601

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Targe Megha
Co-author

Dr. Kolpe Institute of Pharmacy Kolpewadi, kopargaon 423601

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Kuvar Jadhav
Co-author

Dr. Kolpe Institute of Pharmacy Kolpewadi, kopargaon 423601

Nalawade Dipak*, Dhule Poonam, Dhone Suvana, Targe Megha, Kuvar Jadhav, Formulation And Evaluation of Herbal Hair Mask of Wheatgrass for Hair Growth, Int. J. of Pharm. Sci., 2025, Vol 3, Issue 6, 5742-5752. https://doi.org/10.5281/zenodo.15770566

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