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  • Hepatoprotective Effect of Curcumin Microsponges against Paracetamol Induced Liver Toxicity in Rats
  • aDepartment of Pharmacology, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.
    bDepartment of Pharmacy, VADP’s VPD Institute of Pharmacy, Shirsondi Tal. Malegoan Dist. Nashik, India.
    cDepartment of Pharmacology, JES's SND College of Pharmacy, Babulgaon, Tal. Yeola, Dist. Nashik, India.
    dDepartment of Pharmaceutical Quality Assurance, Aldel St. John Institute of Pharmacy and Research, Palghar Dist: Thane, India.
    eDepartment of Pharmacy, SMES’s Mahavir Institute of Pharmacy, Nashik, India.
    fDepartment of Pharmaceutical Chemistry, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.

Abstract

There are currently numerous pharmacological effects associated with curcumin, but its use in clinical settings is constrained by its poor bioavailability. In this research, we created a formulation for curcumin microsponges and assessed their ability to protect rats livers from damage brought on by paracetamol. Paracetamol (2 g/kg/day) was used to produce toxicity over the course of 10 days. Paracetamol causes marked hepatotoxicity in rats by raising the standard of ALT, AST, ALP, and total bilirubin, which are liver function indicators along with reducing total protein level. Animal were treated orally including Curcumin microsponges (100 mg/kg) and plain curcumin (P-CUR) (100 mg/kg) daily for 10 days. Rats were sacrificed on the last day of treatment and the liver was excised for histopathological analysis. According to our findings, microsponges have a greater hepatoprotective impact than regular plain curcumin. When curcumin microsponges were administered, paracetamol-induced liver damage was successfully reduced, as seen by a decrease in liver function indicators. Our study suggests that curcumin microsponges have strong potential hepatoprotective effects.

Keywords

Curcumin, microsponges, paracetamol, biomarkers, liver, hepatoprotective

Reference

  1. Rose W, Jittikoon J, 2001. Vitamin D and liver fibrosis: molecular mechanisms and clinical studies. Biomed Pharmacother. 2019;109:1351-60. doi: 10.1016/j.biopha.2018.10.140, PMID 30551386.
  2. Dinesh K, Sivakumar V, Selvapriya B. Evaluation of hepatoprotective polyherbal formulations contains some Indian medicinal plants. J Pharmacogn Phytochem. 2014;3(4):1-5.
  3. Li J, Fan JG. Characteristics and mechanism of liver injury in 2019 coronavirus disease. J Clin Transl Hepatol. 2020;8(1):13-7. doi: 10.14218/JCTH.2020.00019, PMID 32274341.
  4. Girish C, Koner BC, Jayanthi S, Ramachandra Rao K, Rajesh B, Pradhan SC. Hepatoprotective activity of picroliv, curcumin and ellagic acid compared to silymarin on paracetamol induced liver toxicity in mice. Fundam Clin Pharmacol. 2009;23(6):735-45. doi: 10.1111/j.1472-8206.2009.00722.x, PMID 19656205.
  5. Bessems JK, Vermulen NP. Paracetamol (acetaminophen)-induced toxicity: molecular and biochemical mechnisms, analogues and protective approaches. Crit Rev Toxicol. 2001;2(31):55-138.
  6. Kwan D, Bartle WR, Walker SE. Abnormal serum transaminases following therapeutic doses of acetaminophen in the absence of known risk factors. Dig Dis Sci. 1995;40(9):1951-5. doi: 10.1007/BF02208663, PMID 7555449.
  7. Coen M. Metabolic phenotyping applied topre-clinical and clinical studies of acetaminophen metabolism and hepatotoxicity. Drug Metab Rev. 2014;23:1-16.
  8. Ojo OO, Kabutu FR, Bello M, Babayo U. Inhibition of paracetamol induced oxidative stress in rats by extracts of lemongrass (Cymbropogon citratus) and green tea (Camellia sinensis) in rats. Afr J Biotechnol. 2006;5:1227-32.
  9. Abu-Rizq HA, Mansour MH, Afzal M. Curcuma longa attenuates carbon tetrachloride-induced oxidative stress in T-lymphocyte subpopulations. Methods Mol Biol. 2015;1208:159-70. doi: 10.1007/978-1-4939-1441-8_12, PMID 25323506.
  10. Xu Y, Lin D, Li S, Li G, Shyamala SG, Barish PA, et al. Curcumin reverses impaired cognition and neuronal plasticity induced by chronic stress. Neuropharmacology. 2009;57(4):463-71. doi: 10.1016/j.neuropharm.2009.06.010, PMID 19540859.
  11. Yu W, Wu J, Cai F, et al. Curcumin alleviates diabetic cardiomyopathy in experimental diabetic rats. PLOS ONE. 2013;7:52013.
  12. Jain A, Samykutty A, Jackson C, Browning D, Bollag WB, Thangaraju M, et al. Curcumin inhibits PhIP induced cytotoxicity in breast epithelial cells through multiple molecular targets. Cancer Lett. 2015;365(1):122-31. doi: 10.1016/j.canlet.2015.05.017, PMID 26004342.
  13. Luthra PM, Lal N. Prospective of curcumin, a pleiotropic signalling molecule from Curcuma longa in the treatment of Glioblastoma. Eur J Med Chem. 2016;109:23-35. doi: 10.1016/j.ejmech.2015.11.049, PMID 26748069.
  14. Samini F, Samarghandian S, Borji A, Mohammadi G, bakaian M. Curcumin pretreatment attenuates brain lesion size and improves neurological function following traumatic brain injury in the rat. Pharmacol Biochem Behav. 2013;110:238-44. doi: 10.1016/j.pbb.2013.07.019, PMID 23932920
  15. Wu GQ, Chai KQ, Zhu XM, Jiang H, Wang X, Xue Q, et al. Anticancer effects of curcumin on lung cancer through the inhibition of EZH2 and NOTCH. Oncotarget [Epub ahead of print]. 2016;7(18):26535-50. doi: 10.18632/oncotarget.8532, PMID 27049834.
  16. Bao W, Li K, Rong S, Yao P, Hao L, Ying C et al. Curcumin alleviates ethanol-induced hepatocytes oxidative damage involving heme oxygenase-1 induction. J Ethnopharmacol. 2010;128(2):549-53. doi: 10.1016/j.jep.2010.01.029, PMID 20080166
  17. Nabavi SF, Daglia M, Moghaddam AH, Habtemariam S, Nabavi SM, 2008. Curcumin and liver disease: from chemistry to medicine. Compr Rev Food Sci Food Saf. 2014;13(1):62-77. doi: 10.1111/1541-4337.12047, PMID 33412694 (properties of curcumin. Chem. Biol. Interact., 174).
  18. García-Niño WR, Pedraza-Chaverrí J. Protective effect of curcumin against heavy metals-induced liver damage. Food Chem Toxicol. 2014;69:182-201. doi: 10.1016/j.fct.2014.04.016, PMID 24751969.
  19. Ornchuma N, Raymond MS, Wim EH (2014) Curcumin nanoformulations: a review of pharmaceutical properties and preclinical studies and clinical data related to cancer treatment. Biomaterials. 2014;35(10):3365-83. doi: 10.1016/j.biomaterials.2013.12.090, PMID 24439402
  20. Prasad S, Tyagi AK, Aggarwal BB. Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. Cancer Res Treat. 2014;46(1):2-18. doi: 10.4143/crt.2014.46.1.2, PMID 24520218.
  21. Siviero A, Gallo E, Maggini V, Gori L, Mugelli A, Firenzuoli F et al. Curcumin, a golden spice with a low bioavailability. J Herb Med. 2015;5(2):57-70. doi: 10.1016/j.hermed.2015.03.001.
  22. Osmani RA, Aloorkar NH, Ingale DJ, Kulkarni PK, Hani U, Bhosale RR et al. Microsponges based novel drug delivery system for augmented arthritis therapy. Saudi Pharm J. 2015;23(5):562-72. doi: 10.1016/j.jsps.2015.02.020, PMID 26594124.
  23. Jangde R. Microsponges for colon targeted drug delivery system: an overview. Asian J Pharm Technol. 2011;1:87-93.
  24. Amrutiya N, Bajaj A, Madan M. Development of microsponges for topical delivery of Mupirocin. AAPS PharmSciTech. 2009;10(2):402-9. doi: 10.1208/s12249-009-9220-7, PMID 19381834.
  25. Jain V, Singh R. Dicyclomine-loaded Eudragit®-based microsponge with potential for colonic delivery: preparation and characterization. Trop J Pharm Res https :. 2010;9(1). doi: 10.4314.
  26. Lahon K, Das S. Hepatoprotective activity of Ocimum sanctum alcoholic leaf extract against paracetamol-induced liver damage in Albino rats. Pharmacogn Res. January 2011;3(1):13-8. doi: 10.4103/0974-8490.79110, PMID 21731390.
  27. Sayed MM, El-Kordy EA. The protective effect of curcumin on paracetamol-induced liver damage in adult male rabbits: biochemical and histological studies. Egypt J Histol. 2014;37(4):629-39. doi: 10.1097/01.EHX.0000455822.82783.4b.
  28. Du K, Ramachandran A, Jaeschke H, 2016. Oxidative stress during acetaminophen M.M. Ahmad, et al. Gene. hepatotoxicity: sources, pathophysiological role and therapeutic potential. Redox Biol 10. 2019 143966;712:148-56.
  29. Farghaly HS, Hussein MA. Protective effect of curcumin against paracetamol-induced liver damage. Aust J Basic Appl Sci. 2010;4(9):4266-74.
  30. Maiti K, Mukherjee K, Gantait A, Saha BP, Mukherjee PK. Curcumin–phospholipid complex: preparation, therapeutic evaluation and pharmacokinetic study in rats. Int J Pharm. 2007;330(1-2):155-63. doi: 10.1016/j.ijpharm.2006.09.025, PMID 17112692.
  31. Mc Gill MR, Jaeschke H. Metabolism and disposition of acetaminophen: recent advances in relation to hepatotoxicity and diagnosis. Pharm Res. 2013;30(9):2174-87. doi: 10.1007/s11095-013-1007-6, PMID 23462933.
  32. Islam R, Alam J. Evaluation of liver protective activity of Moringa oleifera bark extract in paracetamol induced hepatotoxicity in rats. bioRxiv. 2019.
  33. Eldutar E, Kandemir FM, Kucukler S, Caglayan C. Restorative effects of chrysin pretreatment on oxidant–antioxidant status, inflammatory cytokine production, and apoptotic and autophagic markers in acute paracetamol-induced hepatotoxicity in rats: an experimental and biochemical study. J Biochem Mol Toxicol. 2017;31(11):e21960. doi: 10.1002/jbt.21960, PMID 28682524.
  34. Hamid A, Lee LS, Karim SR, Jufri NF. Hepatoprotective effects of zerumbone against paracetamol-induced acute hepatotoxicity in rats. Malays J Med Sci. 2018;25(2):64-71. doi: 10.21315/mjms2018.25.2.7, PMID 30918456.
  35. Yousef MI, Omar SAM, El-Guendi MI, Abdelmegid LA. Potential protective effects of quercetin and curcumin on paracetamolinduced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food Chem Toxicol. 2010;48(11):3246-61. doi: 10.1016/j.fct.2010.08.034, PMID 20804811.
  36. Kannan N, Sakthivel KM, Guruvayoorappan C. Protective effect of Acacia nilotica(L.) against acetaminophen-induced hepatocellular damage in Wistar rats. Adv Pharmacol Sci. 2013;2013:987692. doi: 10.1155/2013/987692, PMID 23864853.

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Gaurav Kasar
Corresponding author

Department of Pharmacology, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.

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Pooja Rasal
Co-author

Department of Pharmacology, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.

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Ritesh Khairnar
Co-author

Department of Pharmaceutical Quality Assurance, Aldel St. John Institute of Pharmacy and Research, Palghar Dist: Thane, India.

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Revati Khairnar
Co-author

Department of Pharmacy, SMES’s Mahavir Institute of Pharmacy, Nashik, India.

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Shubham Khaire
Co-author

Department of Pharmacology, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.

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Yunus Ansari
Co-author

Department of Pharmaceutical Chemistry, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.

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Manoj Mahajan
Co-author

Department of Pharmacology, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.

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Aman Upaganlawar
Co-author

Department of Pharmacology, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.

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Amol Thakare
Co-author

Department of Pharmacy, VADP’s VPD Institute of Pharmacy, Shirsondi Tal. Malegoan Dist. Nashik, India.

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Chandrashekhar Upasani
Co-author

Department of Pharmacology, SNJB’s Shriman Sureshdada Jain College of Pharmacy, Chandwad Dist: Nashik, India.

Gaurav Kasarab, Pooja Rasal, Ritesh Khairnar, Revati Khairnar, Shubham Khaire, Yunus Ansari, Manoj Mahajan, Aman Upaganlawar, Amol Thakare, Chandrashekhar Upasani, Hepatoprotective Effect of Curcumin Microsponges against Paracetamol-Induced Liver Toxicity in Rats, Int. J. of Pharm. Sci., 2024, Vol 2, Issue 1, 841-856. https://doi.org/10.5281/zenodo.10590649

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