Paramhansa Ramkrishna Maunibaba Shikshan Sanstha's Anuradha College of Pharmacy, Anuradha Nagar, Sakegaon Road, Chikhli, Buldhana – 443201
Hair loss, particularly androgenic alopecia, is a widespread condition affecting individuals’ physical appearance and psychological well-being. The present study focuses on the development and evaluation of a polyherbal hair oil formulated using natural plant-based ingredients. The formulation includes medicinal plants such as Amla, Aloe vera, Garlic, Hibiscus, Fenugreek, Neem, and Curry leaves incorporated in a suitable oil base. The prepared formulation was evaluated for physicochemical parameters including pH, viscosity, specific gravity, acid value, and stability. The study concludes that polyherbal hair oil can effectively reduce hair fall, improve scalp condition, and promote hair growth.
Hair is an important component of the human body that contributes significantly to appearance and self-confidence. Alopecia is defined as partial or complete loss of hair and can occur due to genetic, hormonal, or environmental factors. Hair growth follows three phases: anagen, catagen, and telogen.
The Main function of hair Follicle is to: -
1) To promote growth of hair
2) Help to repair skin after the wound or an injury.
3) Forms the new blood vessels.
4) Form new neuron cells of nervous system (neurogenesis).
Benefits of Herbal Hair Oil: -
• The hair oil helps prevent hair loss and reduces frizz. The ends of the human hair need special care, and vegetable oil help to pampers the throughout the diet.
• Gives shine to hair.
• It helps to relieve stress.
• Regular use of hair oils helps to cure the problem of premature grey hair.
Ideal characteristics of Hair oil: -
• It must impart pleasant smell to the hair.
• It should not have any side effects.
• It must be fluently removed on irrigating with the water.
• It should give luster to the hair.
MATERIALS AND METHODS
1) Measure Ingredients: Weigh all the ingredients in given formula accurately.
2) First of all, the oils (Coconut, Rosemary, Castor, Almond oil, Pumpkin seed oil, Gulmehendi oil) given in formula are mixed together in clean, dry pan
3) Heat the mixture of base oil on low temperature (below 60ºC) for about 30 mins
4) Add the herbal powders to the mixture of oil and mix thoroughly to ensure all the powders are evenly dispersed in the oil.
5) The low heat will help to release the active ingredients from the powders without damaging their properties.
6) Heating is continued till the oil is infused and has taken on the color and aroma of the herbs, allow it to cool to room temperature.
7) After oil is cool at room temperature add mixture of juices (Aloe, Ginger, onion, neem, lemon, Spinach, Licorice root, Centella asiatica) in prescribed quantity in formula.
8) Again, heat the oil at low temperature for nest 60 minutes again so that all the ingredients in juices form that are concentrated and enhance the medicinal property of oil.
9) After concentrating the juices after heating then switch off the hot plate wait for normalize the temperature of oil to room temperature
10) Strain the oil through a muslin cloth or fine strainer into a clean container.
11) Ensure you squeeze the cloth well to extract as much oil as possible.
12) Add vitamin E oil (1-2 capsules per 200 ml) to act as a natural preservative.
13) Pour the strained oil into a dark, airtight glass bottle to protect it from light and extend its shelf life.
Evaluation Parameters
Organoleptic properties (color, odor)
pH determination
Viscosity measurement
Specific gravity
Acid value
Saponification value
Skin irritation test
Sensitivity test
The prepared polyherbal hair oil was applied on 1 cm skin of hand it is exposed to the sunlight 4 -5 min.
Acid Value
Preparation of 0.1 M solution: 0.56 g of KOH pellets weighed and dissolved in 100 ml of distilled water and stirred it continuously. The prepared 0.1 M KOH solution was filled in burette.
Preparation of sample: Measure 10 ml oil and dissolved in 25 ml of ethanol and 25 ml of ether and shaken well .1 ml of phenolphthalein indicator is added and titrated with 0.1 M KOH solution.
Saponification Value
Weighed accurately 1 ml of oil into 250 ml of conical flask and 10 ml ethanol: ether mixture (2:1) was added. To this flask 25 ml of 0.5 N alcoholic KOH was added. The flask was kept aside for 30 min. The cooled solution was titrated against 0.5 N HCl using phenolphthalein indicator. Similarly, the blank titration was performed without taking oil (sample). Amount of KOH in mg used was calculated.
pH measurement
The pH of prepared polyherbal hair oil was determined using pH meter.
Viscosity
The viscosity of polyherbal hair oil was determined using Ostwald's Viscometer.
RESULTS
• Physical Evaluation: -
Table no.1.Results of physical evaluation
|
Sr. No |
Parameters |
Observation |
|
1 |
Colour |
Greenish brown |
|
2 |
Odour |
Characteristics |
|
3 |
Specific gravity |
1.093 |
|
4 |
Viscosity |
0.961 poise |
|
5 |
pH |
6.5 |
|
6 |
Acid value |
3.5 |
|
7 |
Saponification value |
115.05 |
|
8 |
Irritation test |
No irritation |
|
9 |
Refractive index |
1.402 |
Phytochemical test for polyherbal hair oil: -
Table no.2.Results of phytochemical test
|
Sr. No |
Name of the test |
Results |
|
1 |
Alkaloids |
-ve |
|
2 |
Flavonoids |
+ve |
|
3 |
Steroids and terpenoids |
+ve |
|
4 |
Glycosides |
+ve |
|
5 |
Tannins |
+ve |
|
6 |
Saponins |
-ve |
|
7 |
Phenols |
+ve |
Table.no.3. Formulation of polyherbal hair oil
|
Sr. No |
Ingredients |
F1 |
F2 |
F3 |
|
1 |
Amla |
5 |
3 |
4 |
|
2 |
Almond oil |
2 |
2 |
1 |
|
3 |
Garlic |
1 |
0.5 |
1 |
|
4 |
Henna powder |
2 |
1 |
2 |
|
5 |
Neem leaves |
2.5 |
5 |
3.5 |
|
6 |
Curry leaves |
2.5 |
3.5 |
3.5 |
|
7 |
Castor oil |
20 |
30 |
15 |
|
8 |
Coconut oil |
25 |
15 |
30 |
DISCUSSION
The formulation and evaluation of polyherbal hair oil using Neem leaves, curry leaves, tulsi leaves, hibiscus flowers, fenugreek seeds, was prepared. The various parameters like color, odour, irritation test, sensitivity test, viscosity, pH of herbal hair oil evaluated. The formulated herbal hair oils exhibited promising physicochemical properties, antioxidant activity, antimicrobial efficacy, safety, and hair growth promotion in animal models. These findings suggest their potential as natural remedies for hair care. Herbal cosmetics, particularly herbal hair oils, have gained popularity due to their perceived efficacy and minimal adverse effects. This study aimed to formulate and evaluate herbal hair oils for promoting hair growth.
Methodology: Various herbal extracts, including Coconut, Curry leaves, Amla, Garlic, Fenugreek, and Onion, Hibiscus were prepared and incorporated into virgin Coconut oil. Phytochemical screening, total flavonoid content, organoleptic evaluation, chemical analysis (acid value, saponification value, specific gravity), stability studies, antioxidant assay (DPPH), antimicrobial assay, sensitivity studies, and in-vivo hair growth activity were conducted. The formulation of a polyherbal hair oil for the treatment of alopecia has been undertaken in this study, focusing on the utilization of natural herbs known for their hair growth properties. Extensive research was conducted to identify and gather different herbs from natural sources, as they contain various phytochemicals and nutrients that promote hair growth. The process involved herb collection, extraction, phytochemical screening, and formulation evaluation. Essential constituent in herbs which blocks the DHT (Dihydrotestosterone),5 alpha reductase and by nourishing the hair providing nutrient they promote hair growth.
CONCLUSION
Polyherbal hair oil is a safe and effective solution for managing hair loss. It provides a natural alternative to synthetic products with minimal side effects. The formulated polyherbal hair oil shows the better hair growth activity with less or no side effects as the essential phytochemicals are present in the herbs that are required for the hair growth activity, and it also treats the different types of alopecia. The present work of formulation has anti-dandruff, moisturizing and anti-microbial activity. The herbs used in the formulation also stimulates hair follicles and therefore helps regrowing hairs.
REFERENCES
Vaibhav Dhengle, Uddhav Awakale, Rutuparna Talekar*, Raju Muley, Sachin Ambhore, Sachin Kale, K. R. Biyani, Research on Formulation & Evaluation of Herbal Hair Oil, Int. J. of Pharm. Sci., 2026, Vol 4, Issue 5, 976-980. https://doi.org/10.5281/zenodo.20044934
10.5281/zenodo.20044934
