JBVP’S Vidya Niketan College of Pharmacy, Lakhewadi.
The goal of the study was to create herbal biscuits with fiber (made from holy basil and moringa) and enough nutrients (enhanced with fiber and protein). Making herbal biscuits is a cutting-edge, futuristic study in the realm of preparing useful foods. Castor Sugar, Brown Sugar, ragi flour, milk, Melted Butter, salt, baking soda, baking powder, sugar, vanilla extract, Beetroot powder were used to make wheat flour-based herbal biscuits for this study. Six white butter biscuit samples were made, varying in the amount of ragi flour and Beetroot powder. All six samples underwent sensory analysis using a 9-point hedonic scale. Because of its flavor, the cookie is approved at 2?etroot Powder and 28% ragi. Additionally, ghee was used to enhance the flavor of the biscuits instead of Melted butter. The process for making biscuits was repeated, and the same percentage composition was used for ghee biscuits as well. The samples of Melted butter underwent physical and sensory investigation. The analysis shows that the biscuits prepared from more diameter and thickness and significantly got the highest score in all the aspects of sensory analysis. biscuits are better in context with their nutritional benefits than butter. The health benefits of herbal biscuits might entice consumers to eat them for a healthy, balanced diet by appealing to their taste buds. Thus, creating and using these functional meals will benefit people with a variety of illnesses in addition to improving the population's nutritional status
Beetroot is a herbaceous plant that grows every two years. Vitamins, minerals, antioxidants, and dietary fiber can all be found in beetroot and leaves. In order to preserve its quality, beet root, which is frequently consumed by humans, should be kept under controlled circumstances at a temperature between 0 and 5 C, in a dark location, and with a relative humidity between 96 and 100%. Beetroot can be kept for three to five months under these conditions [1]. A maximum of 5–7% weight loss during storage is acceptable; however, greater losses degrade the nutritional value of the beetroot [1]. Fresh beetroot can be dried at low temperatures to preserve its nutritional qualities for an extended period of time. (50 C for 6 hours), then crushed into a powder and stored in tightly sealed containers. Beetroot can be added in this form to a variety of food products to improve their nutritional value and health-promoting qualities [2]. The market for readily consumable functional meals has grown significantly in recent years, which has prompted producers to create novel, nutrient-dense goods with positive health effects. This can prevent or delay the onset of numerous diseases brought on by inadequatenutrition, including diabetes, cardiovascular disease, and some forms of cancer, in addition to lowering obesity [3].
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Fig 1: Overview Of Potentially Bioactive Compounds in Beetroot
Dietary fiber is the endogenous components of plant material in the diet which are resistant to digestive enzymes produced by man. Dietary fiber is an important part of a healthy diet. It helps move food and waste efficiently through the digestive system [4]. Fiber is the part of plant foods that cannot be broken down by human digestive enzymes in the small intestine [5]. Fiber is mostly complex carbohydrates. The two types of fiber are soluble and insoluble. While they work differently, both are needed for proper bowel function. Most fiber sources contain both kinds of fiber in varying amounts. Insoluble Fiber cannot be dissolved in water. This type of fiber attracts water to the intestines, making stools bulky and soft. It also speeds the movement of food through the digestive tract. Therefore, insoluble fiber may help prevent diverticular disease, colon cancer, hemorrhoids, and constipation. Cellulose, hemi cellulose and lignin are insoluble fibers. They produce the tough, chewy texture of wheat bran, whole grains, corn bran, and some vegetables [6] Soluble fiber, or fiber that can dissolve in water, slows the movement of food through the body but does not increase fecal bulk. Soluble fiber helps maintain a healthy cholesterol level, normalize blood sugar levels in diabetics and may even help reduce blood pressure. Pectins and gums are examples of soluble fibers, and they are also found in herbs like Tulshi (Ocimum sanctum), Moringa Leaves (Moringa oleifera), beans, oat bran, psyllium husks, Apple, Apricot, Carrots, Yams and some fruits and vegetables [7,8]. Dietary fiber is essentially intact within the plant matrix, and the enzymes within the human digestive tract are unable to hydrolyze or break it down. Dietary fiber is made up of three varying components. The largest component consists of polysaccharides, or plant fibers such as bran; pectins from fruits and vegetables; various gums; and beta glucans from oats and rye [7]. Dietary fiber is made up of naturally occurring plant materials that are resistant to humanproduced digestive enzymes. Dietary fiber holds significant importance in a nutritious diet. It facilitates the digestive system's effective passage of food and waste [8]. Fiber is the component of plant foods that cannot be broken down by human digestive enzymes in the small intestine [9]. Most complex carbs make up fiber. Soluble and insoluble fibers are the two different kinds. Despite their differing modes of operation, both are essential for healthy colon function. Both types of fiber are present in varied levels in the majority of fiber sources. Water cannot dissolve insoluble fiber. This kind of fiber draws water into the digestive tract, resulting in soft, thick stools. Additionally, it expedites the food's passage through the digestive system. Thus, insoluble fiber may aid in the prevention of constipation, hemorrhoids, colon cancer, and diverticular disease. Insoluble fibers include lignin, hemicellulose, and cellulose. They provide wheat bran, whole grains, corn bran, and some vegetables their coarse, chewy texture [10]. In diabetics, soluble fiber helps to balance blood sugar levels, maintain a healthy cholesterol level, and may even assist lower blood pressure. Soluble fibers include things like pectins and gums, which can be found in some fruits and vegetables, beans, oat bran, psyllium husks, tulshi (Ocimum sanctum), carrots, yams, and herbs like Moringa leaves (Moringa oleifera)[11,12]. The plant matrix contains dietary fiber that is largely intact and cannot be hydrolyzed or broken down by the enzymes in the human digestive system. There are three distinct parts to dietary fiber. Polysaccharides, or plant fibers like bran, pectins from fruits and vegetables, different gums, and betaglucans from rye and oats make up the majority of the mixture [12]. The nutritional benefits, palatability, convenience, and compactness of biscuits make them perfect [13]. Biscuits often have a longer shelf life and are less susceptible to microbial deterioration because to their lower moisture content than cakes and bread [14]. The goal of the current study was to create a product with a low calorie count and a high fiber content. The nutritional benefits, palatability, convenience, and compactness of biscuits make them perfect [13]. Biscuits often have a longer shelf life and are less susceptible to microbial deterioration because to their lower moisture content than cakes and bread [14].
MATERIALS AND METHODS:
Place of Experiment
The samples analyses were conducted in the laboratory of Vidya Niketan College of Pharmacy, Lakhewadi, Pune In Analytical Pharmaceutics department, Dr. Babasaheb Ambedkar University, Lonare. Protein and fat tests of different samples were conducted in Institute of Vidya Niketan College of Pharmacy Lakhewadi,Pune.
Ingredients required
Brown Sugar, castor Sugar, Beetroot Powder, Ragi flour, Sugar, Melted butter, Dark Chocolate, Salt, Vanilla essence, Baking powder, Baking soda. Process of Biscuit Manufacturing: Ingredient was used for preparation of biscuits according to above stated recipe.
Machines and apparatus required
Microwave oven, Weighing machine, Blenders, Digital vernier caliper, Sieve, Rolling pin, Rolling board, Cutter.
Sensory Analysis of the Formulated Herbal Biscuits
Sensory analysis plays a key role in the development of foods and beverage for consumption (Moskowitz, 1984). Sensory evaluation of the biscuits was carried out by panel of 30 person (students and staff) to whom the score cards were given. The different characteristics which were used for evaluating biscuits were appearance, taste, texture, flavour and colour. The scores of the score card ranked from five to one. Nutrient Analysis of the Selected Biscuits Volume: 3; Issue: 4; April-2017; ISSN: 2454-5422 The selected biscuits using sensory analysis were analyzed for protein, fibre and iron
Biochemical Quality Analysis
All the samples were subjected to chemical quality assessment. Moisture was determined by oven-drying method, Ash was determined by incineration method, Fat was determined by soxhlet appratus method.
Moisture Content
Moisture generally refers to the presence of water often in trace amounts. The moisture content is often an importance aspect because its presence in excess amount can promote bacterial growth, decay, moulding or rotting over time. Hence, moisture content of the selected biscuits were analyzed using standard procedure.
Peroxide Value
Peroxide values of the selected products were analyzed by Idometric method (2002).
Microbial Analysis
Microbial study was done to check the growth of micro-organism after a period of storage. Pour plate technique spread plate techniques were carried-out to find the total plate count at room temperature.
Physical analysis
Physical parameters are computed from the following formulas:
Spread ratio: The spread ratio was determined by using this formula.
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Thickness: The thickness was measured in mm by digital vernier caliper.
Volume: The volume of biscuits is defined as the area of the biscuit multiplied by thickness.
Diameter: The diameter was measured in mm by digital vernier caliper.
Density: After calculating volume, density was obained by the ratio of the weight of volume.
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Methods:
The flow chart for the preparation of Herbal Biscuit is shown Figure.1
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Figure-1. Flow Chart For The Development Of Herbal Biscuits For making biscuits in accordance with the recipe mentioned above. [Figure.1]
To create a homogenous mixture, sieve the refined wheat flour and add the remaining dry ingredients.
RESULTS AND DISCUSSIONS
Comparison of Organoleptic or Sensory Qualities of Biscuits: Organoleptic tests of the biscuits depend on its first appearance, color, flavor & smell, texture, and overall taste of the sample. Table-2 shows the comparison among the biscuits of their organoleptic quality factors.
Quality Parameters |
Normal biscuits |
Sample-1 |
Sample-2 |
Sample-3 |
Points |
Points |
Points |
Points |
|
Mean ±SD |
Mean ±SD |
Mean ±SD |
Mean ±SD |
|
Appearance |
4.07± 0.26 |
4.08 ± 0.17 |
3.05± 0.16 |
3.08± 0.12 |
Color |
4.27 ± 0.70 |
4.17 ± 0.05 |
4.05 ± 0.40 |
3.17 ± 0.57 |
Flavor & Smell |
3.73 ± 0.80 |
3.53 ± 0.70 |
4.13 ± 0.56 |
3.73 ± 0.65 |
Texture |
4.40 ± 0.74 |
4.20 ± 0.56 |
4.05 ± 0.14 |
3.25 ± 0.75 |
Overall taste acceptance |
4.46 ± 0.63 |
4.43 ± 0.53 |
4.03 ± 0.43 |
4.14 ± 0.57 |
Preparation of Herbal Biscuit
During the biscuit preparation, the sugar and fat (white butter/ghee) were blended in a blender to give a creamy combination earlier than the flour and other dry ingredients were added. Further, the mixture was properly mixed to form a consistent dough. The dough was thoroughly kneaded manually for 5 min. The dough was rolled on a rolling board with a rolling pin to a uniform thickness of about 6 mm and cut them into round shapes for equal sizes of diameter 70 mm. The cut-out biscuit dough pieces were kept in a greased baking tray and baked in a hot oven (180?C) for 25 min. The biscuit was cooled without delay after baking and kept in an airtight container and stored at room temperature until used for physical analysis and sensory evaluation. The ragi flour and Beetroot powder were mixed in various dosage recommendations vary depending on the condition being treated. But generally, for a healthy person the recommended dosage should be from 1% to 10%. Other ingredients such as ghee, sugar, milk, salt, vanilla essence, baking powder, and baking soda were kept constant for all six combinations. Biscuits with different composition of ingredients were made for getting the right composition. Due to the bitter taste Beetrooty Powder the amount of Beetroot powder was decreased to acquire better taste by making six samples of biscuits keeping all the ingredients constant and varying the amount of Beetroot and ragi in every sample.
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Six Samples of Herbal Biscuit
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Sensory evaluation of six samples of herbal biscuit and overall acceptability among six samples of herbal biscuit.
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Biscuits Before Baking
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Biscuits After Baking
CONCLUSION
Six white butter biscuit prototypes were made using different amounts of Ragi flour and Beetroot powder. The composition of 30% wheat flour, 28% ragi flour, 20% sugar, 15% white butter, and 2% Beetropot Powder was determined to be highly satisfactory following the fabrication of six samples. For the second biscuit flavor, Melted butter to enhance the flavor. Ghee underwent the same process and had the same constituent makeup.The samples of white butter and ghee (2% Beetroot powder and 28% ragi) are next subjected to sensory and physical investigation. According to the analysis, the biscuits were thicker and larger in diameter, and they also received the top score across the board for sensory analysis. As a result, adding ragi flour and Beetroot powder to biscuits will improve their nutritional value. The creation of these biscuits will assist treat a variety of illnesses in addition to enhancing their nutritional state.
REFERENCES
Amit Jadhav*, Sanika Sarode, Samiksha Chaher, Kalyani Sahare, A Detailed Pharmacological Review on Clitoria Ternatea, Int. J. of Pharm. Sci., 2025, Vol 3, Issue 4, 2994-3001 https://doi.org/10.5281/zenodo.15276623