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  • Antimicrobial Resistant Pattern of Klebsiella pneumoniae Isolated from the Stool of Healthy Volunteers of Niger Delta University, Wilberforce Island, Amassoma, Bayelsa State
  • 1,3Department of pharmaceutical microbiology and biotechnology, faculty of pharmacy, niger delta university, wilberforce island, bayelsa, nigeria.
     2Department of pharmaceutical and medicinal chemistry, faculty of pharmacy, niger delta university, wilberforce island, bayelsa nigeria

Abstract

Background: Klebsiella pneumoniae bacteria is a normal flora of the human intestinal tract where they do not cause disease in normal circumstances but can also act as a human opportunistic pathogenic infection when it proliferates in increased amounts, where it may cause a host of health complaints and symptoms ranging from mild to serious infections (pneumonia, septicemia, Urinary tract infections). The virulence factors like capsule, lipopolysaccharide, and type 1 or type 3 fimbriae are responsible for K. pneumoniae to form biofilm. Klebsiella pneumonia responsible for serious outbreaks of multi-drug resistant diseases may be due to uncontrolled usage of antibiotics. Method: The samples were isolated and identified using standard microbiological methods. The isolates were screened for possible virulence traits using the Blood agar test and Congo-Red test. Antibiotic susceptibility screening was carried out for the isolates. Results: In this study (25.3%) Klebsiella pneumoniae isolates were recovered from the 300 stool samples of the healthy student volunteers, of which 29(38.2%) were from males and 47(61.8%) from females. The 76 Klebsiella pneumoniae isolates screened are biofilm producers while none produced hemolysin. The antimicrobial susceptibility pattern for the 76 Klebsiella pneumoniae isolates in this study revealed (Co-trimoxazole 3.9%, Ciprofloxacin 11.8%, Cefotaxime 9.2%, Ceftazidime 6.6% and Gentamicin 9.2%, Imipenem 17.1% and Ertapenem 40.8% and Nitrofurantoin (89.5%). In this study (17.1%) Klebsiella pneumoniae isolates exhibited multi-drug resistance. Conclusion: Drug resistance surveillance has revealed that asymptomatic carriers in the community are often colonized with resistant bacteria that subsequently lead to infections.

Keywords

Klebsiella pneumoniae, antimicrobial, resistant, pneumonia, septicemia, Urinary tract infections

Reference

  1. Afreenish H, Javid U, Fatima K, Maria O, Ali K and Muhammad I (2011): Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis; 15:1413.
  2. Chung DR, Lee H, Park MH, Jung S-I, Chang -H-H, Kim Y-S, Son JS, Moon C, Kwon KT, Ryu SY, et al. Fecal carriage of serotype K1 Klebsiella pneumoniae ST23 strains closely related to liver abscess isolates in Koreans living in Korea. Eur J Clin Microbiol Infect Dis. 2012 Apr;31(4):481–486. doi:10.1007/s10096-011- 1334-7. 
  3. Clinical and Laboratory Standards Institute (CLSI) performance standards for antimicrobial susceptibility testing. Twenty-fourth Information Supplement. 2014;34(1):M100-S24:67-68.
  4. Cruz-Cordova A, Esteban-Kenel, Espinosa-Mazariego K, Ochoa AS, Espinosa MS, Elhain GA, Rendon FE, Villegas LOE and Xicohtencatl-Cortes J (2014): Pathogenic determinants of clinical Klebsiella pneumoniae strains associated with their persistence in the Hospital environment. Bol. Med. Hosp. Infant. Mex 71: 52-55.
  5. De Campos PA, Royer S, Fonseca DWB (2016): “Multidrug Resistance Related to Biofilm Formation in Acinetobacter baumannii and Klebsiella pneumoniae Clinical Strains from Different Pulsotypes,” Current Microbiology, vol. 72, no. 5, pp. 617–627. 
  6. Esposito EP, Cervoni M, Bernardo M, Crivaro V, Cuccurullo S, Imperi F and Zarrilli R (2018): Molecular Epidemiology and Virulence Profiles of Colistin-Resistant Klebsiella pneumoniae Blood Isolates from the Hospital Agency "Ospedale dei Colli," Naples, Italy. Front Microbiol. 9:1463.
  7. Garner D (2013): Microbiology Nuts and Bolts. Pp 20-22
  8. Gorrie, C. L., Mirc Eta, M., Wick, R. R., Edwards, D. J., Thomson, N. R., Strugnell, R. A., Pratt, N. F., Garlick, J. S., Watson, K. M., Pilcher, D. V., McGloughlin, S. A., Spelman, D. W., Jenney, A. W. J., & Holt, K. E. (2017). Gastrointestinal Carriage Is a Major Reservoir of Klebsiella pneumoniae Infection in Intensive Care Patients. Clinical Infectious Diseases, 65(2), 208–215. https://doi.org/10.1093/cid/cix270
  9. Harris P, Paterson D and Rogers B (2015): Facing the challenge of multidrug-resistant gram-negative bacilli in Australia. MJA ; 202(5):243-247.
  10. Huynh BT, Passet V, Rakotondrasoa A, Diallo T, Kerleguer A, Hennart M, Lauzanne AD, Herindrainy P, Seck A, Bercion R, et al. (2020): Klebsiella pneumoniae carriage in low-income countries: antimicrobial resistance, genomic diversity and risk factors. Gut Microbes. 11(5):1287–1299. doi:10.1080/ 19490976.2020.1748257.
  11. Iredell J, Brown J, and Tagg K, (2016): “Antibiotic resistance in Enterobacteriaceae: mechanisms and clinical implications,” BMJ, vol. 352, p. h6420.
  12. Lathamani K and Subbannayya Kotigadde (2016): Biofilm Formation and its Correlation with ESBL Production in Klebsiella pneumoniae Isolated from a Tertiary Care Hospital. Volume 5 Issue 2: 1059-1062.
  13. Leang K, Sultana I and Takada G (2003).  A novel bioconversion of L-fructose to L-glucose by Klebsiella pneumoniae. J Biosci Bioeng ; 95(3):310-312.
  14. Lee HW, Koh YM, and Kim J (2008): “Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces,” Clinical Microbiology and Infection, vol. 14, no 1, pp. 49–54.
  15. Li, Y., Kumar, S., Zhang, L., & Wu, H. (2022). Klebsiella pneumonia and Its Antibiotic Resistance: A Bibliometric Analysis. In BioMed Research International (Vol. 2022). Hindawi Limited. https://doi.org/10.1155/2022/1668789
  16. Lin YT, Siu LK, Lin JC, Chen T-L, Tseng C-P, Yeh K-M, Chang F-Y, Fung C-P. (2012): Seroepidemiology of Klebsiella pneumoniae colonizing the intestinal tract of healthy Chinese and overseas Chinese adults in Asian countries. BMC Microbiol. 12:13. doi:10.1186/ 1471-2180-12-13. 
  17. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. Multidrug-resistant, extensively drug-resistant and pan drug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol Infect. 2012;18(3):268-81.
  18. Mah TF and O’Toole GA (2001). Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol, 9: 34-39.
  19. Manikandan C. and Amsath A. (2013): Antibiotic susceptibility of bacterial strains isolated from wound infection patients in Pattukkottai, Tamilnadu, India. Int. J. Curr. Microbiol.App.Sci 2(6): 195-203.
  20. Martin R. M. and Bachman M. A, (2018): “Colonization, infection, and the accessory genome of Klebsiella pneumoniae,” Fron tiers in Cellular and Infection Microbiology, vol. 8, p. 4. 
  21. Martin RM, Cao J, Brisse S, Passet V, Wu W, Zhao L, Malani PN, Rao K, Bachman MA, et al. Molecular epidemiology of colonizing and infecting isolates of Klebsiella pneumoniae. mSphere. 2016 Oct 19;1(5): e00261–16. doi:10.1128/mSphere.00261-16.
  22. Masood M, Saba N, Samad A and Qazilbash AA (2002): Klebsiella pneumoniae Urinary tract infections associated with long-term catheterization and Spinal cord injuries. J. Med. Sci, 2 (5-6): 227-229.
  23. Mathai D, Jones RN, Pfaller MA (2001): SENTRY Participant Group North America. Epidemiology and frequency of resistance among pathogens causing urinary tract infections in 1510 hospitalized patients: a report from the SENTRY Antimicrobial Surveillance Program (North America). Diagn Microbiol Infect Dis 2001; 40:129–36.
  24. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T and Rattan A (2006) Detection of biofilm formation among the clinical isolates of Staphylococci: An evaluation of three different screening methods. Indian Journal of Medical Microbiology; 24:25-29. 
  25. Palanisamy M (2015): Antibacterial susceptibility and virulence factors of Klebsiella species isolated from urinary tract infections in Coimbatore, South India. South Indian Journal of Biological Sciences, 1(3); 145-150.
  26. Qin J, Qiu Y, Guo S et al., (2017): “Distribution and antimicrobial resistance profile of Klebsiella pneumoniae,” Chinese Journal of Infection and Chemotherapy, pp. 269–272. 
  27. Raffelsberger, N., Hetland, M. A. K., Svendsen, K., Småbrekke, L., Löhr, I. H., Andreassen, L. L. E., Brisse, S., Holt, K. E., Sundsfjord, A., Samuelsen, Ø., & Gravningen, K. (2021). Gastrointestinal carriage of Klebsiella pneumoniae in a general adult population: a cross-sectional study of risk factors and bacterial genomic diversity. Gut Microbes, 13(1). https://doi.org/10.1080/19490976.2021.1939599
  28. Rao R, Karthika R and Singh S (2008): “Correlation between biofilm production and multiple drug resistance in imipenem resistant clinical isolates of Acinetobacter baumannii,” Indian Journal of Medical Microbiology, vol. 26, no. 4, pp. 333–337.
  29. Selden R, Lee S, Wang WL, Bennett JV, Eickhoff TC. (1971): Nosocomial klebsiella infections: intestinal colonization as reservoir. Ann Intern Med. 74 (5):657–664. doi:10.7326/0003-4819-74-5-657.
  30. Shimasaki T, Seekatz A, Bassis C, Rhee Y, Yelin RD, Fogg L, Dangana T, Cisneros EC, Weinstein RA, Okamoto K, et al. Increased relative abundance of Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae within the gut microbiota is asso ciated with risk of bloodstream infection in long-term acute care hospital patients. Clin Infect Dis. 2019 May 30;68(12):2053–2059. doi:10.1093/cid/ciy796.
  31. Soyege AO, Ajayi AO, Ngwenya E, Basson AK and Okoh AI (2014): Vancomycin and Oxacillin Co-Resistance of Commensal Staphylococci. Jundishapur Journal of Microbiology. 7(4):e9310.
  32. Stanley IJ, Kajumbula H, Bazira J, Kanslime C, Rwego IB and Asiimwe BB (2018): Multi-drug resistance among Escherichia coli and Klebsiella pneumoniae carried in the gut of out-patients from pastoralist communities of Kasese district, Uganda. PLoS ONE 13(7): e0200093.
  33. Vuotto C, Longo F, Balice M, Donelli G and Varaldo P (2014): “Antibiotic resistance related to biofilm formation in Klebsiella pneumoniae,” Pathogens, vol. 3, no. 3, pp. 743–758.
  34. Walter J, Haller S, Quinten C, Kärki T, Zacher B, Eckmanns T, Abu SM, Plachouras D, Kinross P and Suetens C (2018): Ecdc Pps Study Group Healthcare-associated pneumonia in acute care hospitals in European Union/European Economic Area countries: an analysis of data from a point prevalence survey, 2011 to 2012. Euro Surveill. 23(32). 
  35. Weinstein RA, Gaynes R, Edwards JR (2005): National Nosocomial Infections Surveillance System. Overview of nosocomial infections caused by gram-negative bacilli. Clin Infect Dis 41:848–54.

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P. W. Germany
Corresponding author

Department of pharmaceutical microbiology and biotechnology, faculty of pharmacy, niger delta university, wilberforce island, bayelsa, nigeria.

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S. J. Bunu
Co-author

Department of pharmaceutical and medicinal chemistry, faculty of pharmacy, niger delta university, wilberforce island, bayelsa nigeria

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A. A. Onanuga
Co-author

Department of pharmaceutical microbiology and biotechnology, faculty of pharmacy, niger delta university, wilberforce island, bayelsa, nigeria.

P. W. Germany*, S. J. Bunu, A. A. Onanuga, Antimicrobial Resistant Pattern of Klebsiella pneumoniae Isolated from the Stool of Healthy Volunteers of Niger Delta University, Wilberforce Island, Amassoma, Bayelsa State, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 10, 244-253. https://doi.org/10.5281/zenodo.10046187

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