1Department of Pharmaceutical quality Assurance, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212
2Department of Pharmaceutical Chemistry, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212
3Department of Pharmacology, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212
Objective: Objective of the present analytical research work was to develop and validate Spectrophotometric method and High Performance Liquid Chromatographic method (HPLC Method) for the Diacerein bulk and tablet dosage form Methods: A spectrophotometric method and a HPLC method have been developed and validated for estimation of Diacerein in bulk. Method A (UV SPECTROMETRY Method): Methanol was used for the preparation of stock and working standard solutions of the drugs. 400-200nm UV range was used to scanned standard solutions of drugs using UV spectrophotometer. The ?max of Diacerein was found to be 248 nm. Method B (HPLC Method): The HPLC Method for Diacerein was developed using Cosmosil C18 (4.6mm x 250mm, Particle size: 5µm), as stationary particle, isocratic mode. Methanol : Water (80:20v/v) pH3as mobile phase. Mobile phase was maintained at a flow rate of 1 ml/min and detection was carried out at 248 nm. Both the methods were validated in accordance with ICH guidelines Results: Diacerein was found to be linear in the concentration range of 5-25 µg/ml for spectrophotometric and HPLC method. Retention time was found to be 4.3 min for Diacerein. Interpretation and Conclusion: Results of validation study were found to be satisfactory. So, the methods can be successfully applied for the routine analysis of Diacerein.
Amol Amrutkar, Smita Aher, Rishikesh Bachhav, Development And Validation Of Uv-Hplc Method For The Estimation Of Diacerein In Bulk And Tablet Dosage Form, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 2, 94-103. https://doi.org/10.5281/zenodo.6220056