Phytochemistry, Pharmacological Potential, And Traditional Uses of Asparagus Racemosus: A Comprehensive Review

Abstract

Asparagus racemosus (Shatavari) is a widely used medicinal plant known for its adaptogenic, immunomodulatory, anti-inflammatory, antioxidant, antimicrobial, and gastroprotective properties. It belongs to the family Asparagaceae and is rich in bioactive compounds, including steroidal saponins (shatavarins), flavonoids, alkaloids, and polysaccharides, which contribute to its therapeutic potential. This review provides a detailed overview of Asparagus racemosus, including its botanical description, pharmacognostical studies, phytochemical profile, physicochemical analysis, chromatographic techniques, and pharmacological activities. Traditionally, it has been widely used in Ayurveda for female reproductive health, lactation support, digestive health, and stress management. Scientific studies support its role in treating hormonal imbalances, gastric ulcers, diabetes, and neurodegenerative disorders. Chromatographic methods such as TLC, HPLC, and GC-MS have been employed for the identification and standardization of its bioactive constituents. While the plant is generally considered safe, more clinical trials and toxicological studies are needed to confirm its long-term safety and efficacy. Despite its established medicinal applications, research gaps remain in its clinical validation, advanced drug delivery systems, and genetic profiling. The development of nanotechnology-based formulations could enhance its bioavailability and therapeutic effectiveness.

Keywords

Asparagus racemosus, satavri, Phytochemical& pharmacognostical investigation.

Introduction

Asparagus racemosus, commonly known as Shatavari, is a well-known medicinal plant with a rich history in traditional medicine, particularly in Ayurveda, Siddha, and Unani systems. It belongs to the Asparagaceae family and is widely distributed across India, Sri Lanka, and parts of Southeast Asia. This plant thrives in tropical and subtropical climates, often found in sandy soils and at moderate altitudes. Known as the "Queen of Herbs," Shatavari has been traditionally used for its adaptogenic, rejuvenating, and immunomodulatory properties (VM., 2000). The roots of Asparagus racemosus are the most valuable part, used extensively for their therapeutic benefits. They contain various bioactive compounds such as steroidal saponins (shatavarins), flavonoids, alkaloids, and essential nutrients, which contribute to its diverse pharmacological activities. Shatavari is widely recognized for its role in female reproductive health, aiding in hormonal balance, lactation support, and menstrual regulation. Additionally, it has been used to enhance male reproductive health, improve digestion, strengthen immunity, and manage stress-related disorders (Thakur M, 2011). In Ayurveda, Asparagus racemosus is classified as a Rasayana (rejuvenating herb) that helps in maintaining overall health and longevity. Traditionally, it has been used to treat gastric ulcers, inflammation, diarrhea, and respiratory disorders. Modern scientific studies have further validated its medicinal importance, demonstrating its antioxidant, anti-inflammatory, antimicrobial, hepatoprotective, neuroprotective, and anticancer potential. Due to these significant pharmacological properties, Shatavari has gained attention in pharmaceutical and nutraceutical industries (Sharma RK, 2003). Despite its well-documented traditional uses, further research is required to explore its full therapeutic potential, standardize its bioactive components, and ensure its safety through clinical trials. This review provides a detailed analysis of Asparagus racemosus, covering its botanical description, phytochemical composition, pharmacological activities, traditional applications, toxicity profile, and future research prospects (Kamat JP, 2000).

Botanical Description and Taxonomy:

Botanical Description:

Asparagus racemosus is a climbing, perennial shrub belonging to the family Asparagaceae. It is widely distributed in tropical and subtropical regions, particularly in India, Sri Lanka, Nepal, and Southeast Asia. The plant thrives in well-drained, sandy, or rocky soils and is often found in forests, grasslands, and hilly regions at altitudes of up to 1,300 meters. The plant has thin, wiry, and branching stems, which are covered with small, sharp spines. These spines help the plant climb and spread over nearby vegetation. The leaves of Asparagus racemosus are modified into cladodes (leaf-like structures) that are small, needle-shaped, and arranged in a whorled manner. These cladodes help the plant carry out photosynthesis. The true leaves are reduced to small, scale-like structures. One of the most distinctive features of Asparagus racemosus is its tuberous root system. The roots are long, cylindrical, and fleshy, growing in clusters. They are smooth, light brown externally, and white internally, with a mucilaginous texture. These roots are the primary source of medicinal compounds and are harvested for various therapeutic applications (MKR., 1997). The plant produces small, white to pale yellow fragrant flowers arranged in dense racemes. These flowers bloom between June and August and are pollinated by insects. After pollination, the flowers develop into round, fleshy berries that turn purplish-black when mature. Each berry contains one to two seeds, which are black, hard, and slightly wrinkled (Sharma PV, 2001).

Taxonomy of Asparagus racemosus

Asparagus racemosus is classified under the following taxonomic hierarchy:

• Kingdom: Plantae
• Phylum: Angiosperms (Flowering plants)
• Class: Monocotyledons (Monocots)
• Order: Asparagales
• Family: Asparagaceae
• Genus: Asparagus
• Species: Asparagus racemosus

Habitat and Geographical Distribution:

Asparagus racemosus is native to India and is widely distributed across the tropical and subtropical regions of Asia and Africa. In India, it is found in forests and grasslands of Maharashtra, Tamil Nadu, Karnataka, Madhya Pradesh, Uttar Pradesh, and the Himalayan foothills. The plant prefers sandy or loamy soils with good drainage and moderate rainfall. It is drought-resistant and can survive in arid conditions, making it suitable for cultivation in semi-arid regions. Due to its increasing demand in herbal medicine, commercial cultivation has been promoted to prevent overharvesting from the wild (Sairam KS, 2003) (Bhatnagar M S. S., 2006).

Morphological Features

• Roots: Tuberous, succulent, and clustered, light brown externally, white internally, rich in medicinal properties.
• Stem: Climbing, slender, branching, covered with small spines.
• Leaves (Cladodes): Needle-like, green, arranged in clusters, functioning as the main site of photosynthesis.
• Flowers: Small, white or pale yellow, fragrant, blooming in racemes.
• Fruits: Small, round berries, green when unripe, turning purplish-black when mature.
• Seeds: Small, hard, black, enclosed within the berries.

Significance of Botanical Features:

The unique morphological and anatomical features of Asparagus racemosus contribute to its survival and medicinal properties:

• Tuberous roots: The fleshy roots store essential bioactive compounds, making them the most valuable part of the plant for medicinal applications.
• Climbing habit: The plant's ability to climb and spread over surrounding vegetation helps it access sunlight efficiently.
• Cladodes (Modified leaves): Since true leaves are reduced, the plant relies on cladodes for photosynthesis, which helps it survive in dry environments.
• Drought resistance: The plant's ability to thrive in arid and semi-arid regions makes it highly adaptable and cultivable under various conditions (Goyal RK, 2003).

Pharmacognostic Studies:

Pharmacognostic studies of Asparagus racemosus are essential for the identification, authentication, and quality control of the plant. These studies involve macroscopic, microscopic, physicochemical, and phytochemical evaluations to ensure the purity and potency of herbal formulations (Sharma K, 2011).

Macroscopic Characteristics:

Macroscopic evaluation involves the study of external morphological features:

• Roots: Tuberous, long, cylindrical, light brown externally and white internally, with a mucilaginous texture.
• Stems: Slender, wiry, climbing, and covered with small, sharp spines.
• Leaves (Cladodes): Green, needle-like, arranged in a whorled manner, functioning as the main photosynthetic structures.
• Flowers: Small, white to pale yellow, fragrant, occurring in clusters.
• Fruits: Small, round, purplish-black berries containing black, hard seeds (D., 1997).

Microscopic Characteristics:

Microscopic studies help identify cellular structures and tissue arrangements:

• Root Anatomy:

• The outer epidermis consists of a single layer of cells with a thin cuticle.
• The cortex contains parenchymatous cells filled with starch grains and mucilage.
• The vascular bundles are well-developed, with xylem and phloem arranged radially.

• Cladodes (Leaf Anatomy):

• Presence of epidermal cells with thick cuticles for water retention.
• Parenchymatous mesophyll tissue with vascular bundles.
• Stomata are present, helping in gaseous exchange and transpiration (Narendranath KA, Effect of herbal galactogogue (Lactare) a pharmacological and clinical observation, 1986).

Physicochemical Parameters:

Physicochemical analysis helps in standardizing plant materials for herbal formulations:

• Total Ash Value: Indicates the presence of inorganic materials and impurities.
• Acid-Insoluble Ash: Measures the presence of silica and insoluble impurities.
• Water-Soluble Ash: Represents the amount of soluble minerals in the plant.
• Loss on Drying (Moisture Content): Determines the stability and shelf-life of the plant material.
• Extractive Values: Alcohol-soluble and water-soluble extractives help in evaluating the bioactive components present in the plant (Gaitonde BB, 1969).

Phytochemical Screening:

Preliminary phytochemical studies reveal the presence of bioactive compounds responsible for medicinal properties:

• Steroidal Saponins (Shatavarins I–IV): Key bioactive compounds with adaptogenic and hormonal balance properties.
• Flavonoids: Possess antioxidant and anti-inflammatory properties.
• Alkaloids: Show neuroprotective and antimicrobial activities.
• Tannins: Exhibit astringent and wound-healing properties.
• Mucilage and Polysaccharides: Contribute to its demulcent and gastroprotective effects (Nair AGR, 1969).

Phytochemical Profile:

Asparagus racemosus contains a wide range of bioactive compounds that contribute to its medicinal properties. These include steroidal saponins, flavonoids, alkaloids, tannins, mucilage, and essential minerals. These phytochemicals are responsible for the plant’s adaptogenic, anti-inflammatory, immunomodulatory, antimicrobial, and antioxidant effects.

Major Bioactive Compounds:

1. Steroidal Saponins (Shatavarins I–IV):

• The most significant active constituents found in the roots of Asparagus racemosus.
• Shatavarins I–IV have estrogenic and adaptogenic properties, making the plant valuable for reproductive health.
• These saponins contribute to the immunomodulatory effects and overall therapeutic potential of the plant.

2. Flavonoids:

• Possess strong antioxidant and anti-inflammatory properties.
• Include compounds like quercetin, rutin, and kaempferol, which help protect against oxidative stress.
• Support cardiovascular health and neuroprotection.

3. Alkaloids:

• Show antimicrobial and neuroprotective activities.
• Some alkaloids in Asparagus racemosus may have mild sedative and stress-relieving effects.

4. Tannins:

• Exhibit astringent properties and aid in wound healing.
• Have gastroprotective effects, helping in the treatment of ulcers and diarrhea.

5. Mucilage and Polysaccharides:

• Found in high amounts in the roots, contributing to the plant’s demulcent properties.
• Help in soothing the gastrointestinal tract and promoting the healing of ulcers.
• Act as natural prebiotics, supporting gut health.

6. Essential Minerals and Amino Acids:

• The plant is a natural source of calcium, magnesium, iron, and zinc, essential for metabolic functions.
• Contains asparagine, a key amino acid that supports nervous system function (Patricia YH, 2006).

Phytochemical Analysis Methods:

To identify and quantify the bioactive compounds present in Asparagus racemosus, several phytochemical screening techniques are used:

1. Qualitative Phytochemical Tests:

• Foam Test: Detects saponins by the formation of stable froth.
• Shinoda Test: Identifies flavonoids through color changes when treated with magnesium and hydrochloric acid.
• Dragendorff’s Test: Used for the detection of alkaloids.
• Ferric Chloride Test: Identifies tannins by a blue-black or green coloration (Lee do Y, 2009).

2. Chromatographic Techniques:

• Thin-Layer Chromatography (TLC): A preliminary method for separating and identifying different phytochemicals.
• High-Performance Liquid Chromatography (HPLC): Used for quantitative analysis and identification of bioactive compounds with high precision.
• Gas Chromatography-Mass Spectrometry (GC-MS): Helps analyze volatile compounds and essential oils present in the plant (Dalvi S.S, 1990).

3. Spectroscopic Techniques:

• UV-Vis Spectroscopy: Used for determining the concentration of flavonoids and other phenolic compounds.
• Fourier Transform Infrared Spectroscopy (FTIR): Helps in identifying functional groups of bioactive molecules.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Provides structural elucidation of complex phytochemicals (TN, 1994).

Physicochemical Analysis:

Physicochemical analysis is essential for evaluating the quality, purity, and stability of Asparagus racemosus in herbal formulations. These parameters help ensure consistency in pharmaceutical applications and traditional medicine by determining essential characteristics such as moisture content, ash values, extractive values, and pH levels.

Key Physicochemical Parameters:

1. Total Ash Value:

• Represents the total inorganic content in the plant material, including both physiological and non-physiological ash.

• A high ash value may indicate contamination with soil, sand, or other inorganic impurities.

2. Acid-Insoluble Ash:

• Determines the amount of silica or silicate impurities present, which are mostly derived from soil and adhering dust.
• Important for assessing the purity of the plant material.

3. Water-Soluble Ash:

• Measures the portion of total ash that dissolves in water.
• Helps determine the presence of water-soluble minerals and inorganic compounds (. Parihar MS, 2004) (Muruganadan S, 2000).

4. Moisture Content (Loss on Drying):

• Indicates the amount of water present in the plant material.
• High moisture content can promote microbial growth and reduce shelf life (Hannan JM, 2011).

5. Extractive Values:

• Alcohol-Soluble Extractive: Determines the presence of bioactive compounds such as saponins, flavonoids, and alkaloids that are soluble in alcohol.
• Water-Soluble Extractive: Measures the quantity of water-soluble compounds like mucilage, glycosides, and tannins, which contribute to the plant’s medicinal properties.

6. pH Determination:

• The pH value of Asparagus racemosus extracts is crucial for formulating herbal medicines.
• Helps ensure compatibility with pharmaceutical formulations (Ahmad S, 1991).

7. Swelling Index and Foaming Index:

• Swelling Index: Measures the ability of mucilage-rich extracts to absorb water, indicating their demulcent properties.
• Foaming Index: Used to estimate the presence of saponins, which contribute to the plant’s surfactant and medicinal properties (Zhu X, 2010).

8. Bulk and Tap Density:

• Important for evaluating the flow properties of powdered plant material.
• Helps in the formulation of granules and tablets (Sharma S, 1996).

Chromatographic Studies:

Chromatographic techniques are essential for identifying, isolating, and quantifying the bioactive compounds present in Asparagus racemosus. These methods help ensure the standardization, quality control, and pharmacological consistency of herbal formulations. Different chromatographic techniques, such as Thin-Layer Chromatography (TLC), High-Performance Liquid Chromatography (HPLC), and Gas Chromatography-Mass Spectrometry (GC-MS), are used for analyzing the phytochemical constituents of the plant (Mohanta B C. A., 2003).

Thin-Layer Chromatography (TLC):

• A simple and cost-effective method for preliminary screening of phytochemicals.
• Used to separate and identify saponins, flavonoids, alkaloids, and other secondary metabolites.
• A stationary phase (silica gel) and a mobile phase (solvent system) are used to separate compounds based on their polarity.
• The retention factor (Rf) values help compare the presence of specific bioactive compounds (Joglekar GV, 1967).

High-Performance Liquid Chromatography (HPLC):

• A widely used technique for the quantitative and qualitative analysis of phytochemicals.
• Helps in the identification of steroidal saponins like shatavarins, flavonoids, and alkaloids.
• Uses high-pressure pumps to pass a liquid solvent containing the sample mixture through a column filled with an adsorbent material.
• The separated compounds are detected using UV-Vis or fluorescence detectors.

Gas Chromatography-Mass Spectrometry (GC-MS):

• Used for analyzing volatile and essential oil components in Asparagus racemosus.
• GC separates the compounds based on their volatility, while MS helps in their identification using mass-to-charge ratio analysis.
• Useful for detecting terpenes, fatty acids, and other bioactive compounds (Singh KP, 1986).

High-Performance Thin-Layer Chromatography (HPTLC):

• An advanced form of TLC that provides higher resolution and better quantification.
• Can analyze multiple samples simultaneously, making it an efficient method for herbal standardization.
• Uses densitometric scanning for precise identification of bioactive compounds (V. Ashajyothi, 2009).

Ultra-Performance Liquid Chromatography (UPLC):

• A more advanced version of HPLC that allows for faster and more accurate separation of phytochemicals.
• Uses smaller particle size columns, leading to higher resolution and sensitivity.
• Ideal for detecting trace amounts of bioactive compounds in plant extracts (Bhatnagar M, 2006).

Pharmacological Activities:

Asparagus racemosus has been extensively studied for its diverse pharmacological properties, which are attributed to its rich phytochemical composition. The plant exhibits a wide range of therapeutic effects, including adaptogenic, immunomodulatory, anti-inflammatory, antimicrobial, antioxidant, gastroprotective, and neuroprotective activities. These properties make it a valuable medicinal herb in both traditional and modern medicine.

Adaptogenic and Stress-Relieving Activity:

• Asparagus racemosus is classified as an adaptogen, helping the body resist physical, chemical, and biological stressors.
• It modulates cortisol levels and reduces oxidative stress, contributing to its anti-stress and anti-anxiety effects.
• Research suggests its neuroprotective action helps in managing stress-induced disorders, including anxiety and depression (TN., 1995).

Immunomodulatory Activity:

• The plant enhances both innate and adaptive immunity by stimulating immune cells such as macrophages and lymphocytes.
• Shatavarins and polysaccharides present in Asparagus racemosus contribute to its immunostimulatory effects.
• It is traditionally used to strengthen immunity, particularly in postpartum women and individuals with weakened immune systems (] Boger DL, 1985).

Anti-inflammatory and Antioxidant Activity:

• Flavonoids and saponins present in the plant exhibit significant anti-inflammatory activity by inhibiting pro-inflammatory cytokines.
• Antioxidant compounds like quercetin and rutin neutralize free radicals, reducing oxidative damage in cells and tissues.
• These properties make it beneficial in managing chronic inflammatory diseases like arthritis and cardiovascular disorders (Regh NN, 1989).

Antimicrobial and Antifungal Activity

• Asparagus racemosus has shown antibacterial activity against pathogens such as Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.
• It also exhibits antifungal properties against Candida albicans and Aspergillus niger, making it useful in treating fungal infections.
• The presence of alkaloids and flavonoids is linked to its antimicrobial potency (Narendranath KA, 1986).

Gastroprotective and Anti-Ulcer Activity:

• The plant is traditionally used for treating gastric ulcers, acid reflux, and other digestive issues.
• The mucilage content helps protect the gastric mucosa and promotes ulcer healing.
• Studies have shown its ability to reduce gastric acid secretion and increase mucus secretion, offering protection against gastric damage (Shao Y U, 1997).

Neuroprotective and Cognitive-Enhancing Activity:

• Asparagus racemosus has been found to improve memory and cognitive function, making it beneficial in neurodegenerative diseases like Alzheimer’s and Parkinson’s.
• Its antioxidant and anti-inflammatory properties help protect neurons from damage.
• It is used in Ayurvedic medicine for promoting mental clarity and reducing age-related cognitive decline (] Regh NN, 1989).

Female Reproductive Health and Galactagogue Activity:

• One of the most well-known traditional uses of Asparagus racemosus is its role in female reproductive health.
• It acts as a galactagogue, stimulating milk production in lactating mothers.
• Its phytoestrogenic properties help balance hormones and support menstrual health, reducing symptoms of premenstrual syndrome (PMS) and menopause (Placeholder1).

Anti-Diabetic Activity:

• Studies indicate that Asparagus racemosus helps regulate blood sugar levels by improving insulin sensitivity.
• Saponins and flavonoids contribute to its hypoglycemic effects, making it a potential adjunct treatment for diabetes management (Gautam M, 2009).

Cardioprotective Activity

• The plant has been shown to reduce cholesterol levels and prevent lipid peroxidation, supporting cardiovascular health.
• Its vasodilatory and antihypertensive effects help in reducing high blood pressure (SC., 1981) (Mohanta B, 2003).

Traditional & Ayurvedic Uses:

Asparagus racemosus has been a significant medicinal herb in traditional systems of medicine, particularly in Ayurveda, Unani, and Siddha. It has been used for centuries for its rejuvenating, adaptogenic, and therapeutic effects, primarily targeting female reproductive health, digestive disorders, and overall vitality.

Ayurvedic Perspective:

In Ayurveda, Asparagus racemosus is classified as a Rasayana (rejuvenating herb) and is known for its Balya (strength-promoting), Stanyajanana (galactagogue), and Pitta-pacifying properties. It is often recommended for:

• Enhancing female reproductive health, including hormone balance, menstrual cycle regulation, and improving fertility.
• Acting as a galactagogue, increasing milk production in lactating mothers.
• Supporting digestive health by soothing ulcers, reducing acidity, and improving gut function.
• Boosting immunity and reducing stress due to its adaptogenic properties.
• Promoting longevity and vitality as a general health tonic (Sairam KS, 2003).

Traditional Uses in India and Other Cultures:

• Used in Ayurvedic formulations such as Shatavari Kalpa (a herbal preparation for women’s health).
• In Unani medicine, it is considered beneficial for gastrointestinal issues and as a tonic for general weakness.
• In tribal and folk medicine, the root extract is applied for treating wounds, ulcers, and inflammation.
• Traditionally used as a remedy for diabetes, urinary tract disorders, and cough-related ailments (] Gautam M, 2004).

Common Ayurvedic Preparations:

• Shatavari Churna – A powdered form taken with milk or honey for reproductive and digestive health.
• Shatavari Ghrita – A medicated ghee used for improving fertility and lactation.
• Shatavari Asava/Arishta – Fermented herbal formulations used as a tonic for general wellness (Joshi J, 1988).

Future Prospects & Research Gaps:

Despite extensive traditional use and ongoing scientific validation, Asparagus racemosus still presents several research gaps that need further exploration. Future studies can focus on improving its pharmacological understanding, clinical applications, and formulation advancements for better therapeutic efficacy (Takeungwongtrakul S, 2012).

Unexplored Pharmacological Activities

• While Asparagus racemosus is well-documented for its adaptogenic, immunomodulatory, and reproductive health benefits, its potential in neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s) remains underexplored.
• Further investigation is needed into its anti-cancer potential, as preliminary studies suggest cytotoxic effects on certain cancer cell lines.
• More research on its anti-viral activity against emerging infectious diseases could expand its medicinal applications (Pandey SK, 2005).

Clinical Trials and Standardization:

• Limited clinical trials exist to confirm its efficacy and safety in human populations, especially for its role in female reproductive health and gastroprotection.
• Standardization of bioactive compounds (such as shatavarins and flavonoids) is necessary for quality control in pharmaceutical and nutraceutical formulations.
• More comparative studies between synthetic drugs and herbal formulations containing Asparagus racemosus would help establish its place in modern medicine (Christina AJ, 2005).

Advanced Drug Delivery Systems:

• Development of novel drug delivery systems (e.g., nanotechnology, phytosomal formulations) could improve the bioavailability and therapeutic efficacy of Asparagus racemosus extracts.
• Research on sustained-release formulations can help in achieving long-lasting effects, especially in chronic conditions like diabetes and hormonal imbalances (Goel RK, 2006).

Genetic and Biotechnological Advancements:

• The genetic profiling of different strains of Asparagus racemosus can help identify superior varieties with higher therapeutic potency.
• Tissue culture techniques may aid in large-scale propagation and conservation of this medicinal plant, reducing overharvesting from wild sources.
• Studies on metabolic engineering can enhance the biosynthesis of key bioactive compounds, improving the plant’s medicinal potential (Joshi T, 2012).

CONCLUSION:

Asparagus racemosus (Shatavari) is a widely used medicinal plant known for its adaptogenic, immunomodulatory, antioxidant, and reproductive health benefits. Its rich phytochemical composition, including steroidal saponins and flavonoids, contributes to its diverse pharmacological activities. The plant is particularly valued in female reproductive health, digestive wellness, and stress management. While generally safe, further clinical validation and advanced formulations are needed to enhance its efficacy and bioavailability. Integrating traditional knowledge with modern scientific advancements will help maximize its therapeutic potential and ensure its sustainable use in healthcare.

REFRENCES

1. 1. 1. 1. Parihar MS, H. T. (2004). Experimental excitotoxicity provokes oxidative damage in mice brain and attenuation by extract of Asparagus racemosus. J Neural Transm, 1-12.
         2. Boger DL, M. L. (1985). Antimicrobial and cytotoxic properties of 9, 10-dihydrophenanthrenes: structure-activity studies on juncusol. J Med Chem, 1543-1547.
         3.  Gautam M, D. S. (2004). Immunoadjuvant potential of Asparagus racemosus aqueous extract in experimental system. J Ethnopharmacol , 251-255.
         4. Regh NN, N. H. (1989). Immunotherapeutic modulation of intraperitoneal adhesions by Asparagus racemosus. J Postgrad Med, 199-203.
         5. Ahmad S, J. P. (1991). Chemical examination of Shatavari (Asparagus racemosus). Bullet Med Ethnobotanical Res., 157-160.
         6. Bhatnagar M, S. S. (2006). Antisecretory and antiulcer activity of Asparagu racemosus Willd. against indomethacin plus phyloric ligation-induced gastric ulcer in rats. J Herb Pharmacother, 13-20.
         7. Bhatnagar M, S. S. (2006). Antisecretory and antiulcer activity of Asparagus racemosus Willd. Against indomethacin plus phyloric ligation-induced gastric ulcer in rats. J Herb Pharmacother, 13-20.
         8. Christina AJ, A. k. (2005). Antilithiatic effect of Asparagus racemosus Willd on ethylene glycol-induced lithiasis in male albino Wistar rats. Exp Clin Pharmacol, 633-638.
         9. D., S. (1997). he wisdom of healing. new york.
         10. Dalvi S.S, N. P. (1990). Effect of Asparagus racemosus(Shatavari) on gastric emptying time in normal healthy volunteers. J Post Medicine , 91-94.
         11. Gaitonde BB, J. M. (1969). Antioxytocic action of saponi isolated from Asparagus racemosus Willd (Shatavari) on uterine muscle. Arch Int Pharmacodyn, 121-129.
         12. Gautam M, S. S. (2009). Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: implications for immunoadjuvant potential. J Ethnopharmacol. , 241-247.
         13. Goel RK, P. T. (2006). Teratogenicity of Asparagus racemosus Willd. root, an herba medicine. Indian J Exp Biol, 570-573.
         14. Goyal RK, S. J. (2003). Asparagus racemosus an update. Indian J Med Sci, 408-414.
         15. Hannan JM, A. L.-W. (2011). Antihyperglycaemic activity of Asparagus racemosus roots is partly mediated by inhibition of carbohydrate digestion and absorption, and enhancement of cellular insulin action. Br J Nutr., 1-8.
         16. Joglekar GV, A. R. (1967). Galactogogue effect of Asparagus racemosus. Indian Med J , 165.
         17. Joshi J, D. S. (1988). Chemistry of Ayurvedic crude drugs: Part VIIIa-Shatavari-2: Structure elucidation of bioactive Shatavarin-I &other glycosidesb. Indian J Chem, 12-16.
         18. Joshi T, S. S. (2012). Antistress activity of ethanolic extract of Asparagus racemosus Willd roots in mice. Indian J Exp Biol, 419-424.
         19. Kamat JP, B. K. (2000). Antioxdant properties of Asparagus racemosus against damagedinduced by gamma radiation on rat liver mitochondria. J Ethanopharmacol, 425-435.
         20. Lee do Y, C. B. (2009). Anti-inflammatory effects of Asparagus cochinchinensis extract in acute and chronic cutaneous inflammation. . J Ethnopharmacol, 28-34.
         21. MKR., S. (1997). Appendix and indices. Varanasi: Krishnadas aceademy.
         22. Mohanta B, C. A. (2003). Elemental profile in some common medicinal plants of India. Its correlation with traditional therapeutic usage. J Rad Anal Nucl Chem, 175-179.
         23. Mohanta B, C. A. (2003). Elemental profile in some common medicinal plants of India. Its correlation with traditional therapeutic usage. . J Rad Anal Nucl Chem, 175-179.
         24. Muruganadan S, G. H. (2000). Studies on the immunostimulant and antihepatotoxic activities of Asparagus racemosus root extract. . J Med Arom PI Sci, 49-52.
         25. Nair AGR, S. S. (1969). . Occurrence of diosgenin in Asparagus racemosus. Curr Sci , 414.
         26. Narendranath KA, M. S. (1986). Effect of herbal galactogogue (Lactare) a pharmacological and clinical observation. Med Surg, 19-22.
         27. Narendranath KA, M. S. (1986). Effect of herbal galactogogue (Lactare) a pharmacological and clinical observation. Med Surg , 19-22.
         28. Pandey SK, S. A. (2005). . Effect of Asparagus racemosus rhizome (Shatavari) on mammary gland and genital organs of pregnant rat. Phytother Res, 721-724.
         29. Patricia YH, J. A. (2006). Asparinins, asparosides, curillins, curillosides and shavatarins. Structural clarication with the isolation of shatavarin V, a new steroidal saponin from the root of Asparagus racemosus. Tetrahed Lett, 8683-8687.
         30. Regh NN, N. H. (1989). Immunotherapeutic modulation of intraperitoneal adhesions by Asparagus racemosus. J Postgrad Med, 199-203.
         31. Sairam KS, P. N. (2003). Gastroduodenal ulcer protective activity of Asparagus racemosus. An experimental,protective activity of Asparagus racemosus. An experimental,. J Ethnopharmacol, 1-10.
         32. SC., S. (1981). Constituents of the fruits of Asparagus racemosus Willd. Pharmazie, 709.
         33. Shao Y U, P. O. (1997). Steroidal saponins from Asparagus officinalis and their cytotoxic activity. Planta Medica, 258-62.
         34. Sharma K, B. M. (2011). Asparagus racemosus (Shatavari): Aversatile female tonic. Int J Pharm Biol Arch, 855-863.
         35. Sharma PV, C. S. (2001). Chaukhambha orientalis. varanshi.
         36. Sharma RK, D. B. (2003). Charaka samhita-text with english translation and critical exposition based on Chakrapani Datta’s Ayurveda dipika. VARANSHI.
         37. Sharma S, R. S. (1996). Randomized controlled trial of Asparagus racemosus (Shatavari) as a lactogogue in actational inadequacy. Indian Pediatr, 675-677.
         38. Singh KP, S. R. (1986). Clinical trial on Satavari (Asparagus racemosus Willd.) in duodenal ulcer disease. J Res Ay Sid, 91-100.
         39. Takeungwongtrakul S, B. S.-K. (2012). Lipids from cephalothorax and hepatopancreas of Pacific white shrimp (Litopenaeus vannamei): Compositions and deterioration as affected by iced storage. Food Chem, 2066-2074.
         40. Thakur M, T. D. (2011). Improvement of penile erection, sperm count and seminal fructose levels in ivo and nitric oxide release in vitro by ayurvedic herbs. Andrologia., 273-277.
         41. TN, S. (1994). Fukasawa Structure of asparagamine A, a novel polycyclic alkaloid from Asparagus racemosus. Chem Pharm Bull Tokyo, 1360-1362.
         42. TN., S. (1995). TIFFNal structure and relative stereochemistry of a new polycyclic alkaloid, asparagamine A, showing anti-oxytocin activity, isolated from Asparagus racemosus. J Chem Soc, 391-393.
         43. V. Ashajyothi, R. S. (2009). Satyavati, Asparagus racemosus – a phytoestrogen. . Int. J Pharm &Technol, 36-47.
         44. VM., G. (2000). Ayurvedic pharmacology and therapeutic uses of medicinal plants. Mumbai: SPARC.
         45. Zhu X, Z. W. (2010). Hypolipidaemic and hepatoprotective effects of ethanolic and aqueous extracts from Asparagus officinalis L. by-products in mice fed a high-fat diet. J Sci Food Agri, 1129-1135