Samarth institute of pharmacy, belhe-412410, Maharashtra, India
The development and application of materials whose components are located in the nanometer, usually up to 100 nm is referred to as nanotechnology. This study examines sustainable synthesis of silver nanoparticles using egg shells as a new biologically reducing and stabilization agent. The amount of calcium carbonate and other organic ingredients in egg shells provide cost -effective and ecological alternative to conventional methods of chemical synthesis synthesis. The nanoparticles were synthesized by a green approach, probably including a reduction in silver ions (AG+) with an egg extract. The produced nanoparticles were analysed using several characterization techniques, including UV- spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM). Transmission electron microscopy (TEM) was used to assess the size and shape of the particles, and X -ray diffraction (XRD) could be used to examine the crystalline structure. Antimicrobial efficacy of synthesized nanoparticles has been tested against the selection of bacterial and/or fungal strains by established methods such as test diffusion agar or determination of minimum inhibitory concentration (MIC). This research emphasizes the ability to use Biowaste to create valuable nanomaterials that can be applied in many areas such as medicine, agriculture and environmental cleaning.
The synthesis and creation of various nanomaterials is included in a new scientific discipline known as nanotechnology. Chemical and biological methods can be used to create nanoparticles. Chemical synthesis processes were associated with numerous negative effects because they contain harmful chemicals that are absorbed on the surface. Nanoparticles are ecologically benign with chemical and physical processes.(1,2,3,4,5,6.)
Due to low costs, energy efficiency, high production rate and rapid yield of fine homogeneous powder, the SOLGEL chemical process is used to create nanomaterials on a large scale. Atomic force microscopy was used to analysed the structural and chemical characteristics of the material. (9,10)
MATERIAL& METHOD:
Material: Waste egg shell, silver nitrate, distilled water.
Preparation of egg shell powder:
The collected chicken egg shells were thoroughly cleaned with distilled water and dried in the open surroundings. Dry egg shells were ground into a fine powder in a kitchen blender. The egg force gained was a proset and powder with a size <25 µm was stored in the exuscators until it was used
Method:
A number of 3 g of ESP sifted with a concentration of 5 mmol/l was added to 100 ml of Agno3 water solution. The agno3 solution and the mixture maintained at 80 ° C were mixed at a speed of 300 r/min to the magnetic mixer for 24 minutes. Nanoparticles with in situated nanoparticles were filtered, washed several times thoroughly with distilled water and dried. Dried nanoparticles were stored in spill until they were tested.
Fig no:02 Material and Methods
CHARACTERIZATION:
Several characterization methods have been used to analyze various physicochemical NP characteristics. These include methods including particle-size analysis, SEM, TEM, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), infrared (IR) and Brunauer-Emmett-Teller (BET)(17)
Fig no:03 Scanning electron microscopy
ANTI-MICROBIAL TEST:
Silver nanoparticles have proved to be most effective because of good antimicrobial efficacy against E. Coli.
Fig no 04: Anti-microbial tests of silver Nanoparticles
APPLICATION OF SIVER NANOPARTICLES:
Fig no 05: Application of silver
RESULT: Silver nanoparticles synthesized from waste shells and show significant antimicrobial activity against E. coli, S. aureus and P. aeruginosa, K. pneumonia.
DISCUSSION: Synthesis of silver nanoparticles from waste shells is a sustainable and environmentally friendly approach. The antimicrobial activity of these nanoparticles is attributed to their small size and high surface surface. The results suggest that these nanoparticles can be used as an effective antimicrobial agent. Other studies are needed to explore their potential applications in biomedical areas.
CONCLUSION:
In conclusion, the synthesis of silver nanoparticles from waste shells is a viable and sustainable approach. The nanoparticles showed significant antimicrobial activity, making them a promising candidate for biomedical applications. This study emphasizes the potential of waste egg shells as a valuable biomaterial, supports the circular economy and reduces waste. Further research is required to explore the potential of these nanoparticles.
REFERENCES
Pratiksha Dinkar*, Aishwarya Gowda, Shital Gaikwad, Waste to Value: Synthesis and Microbial Evaluation of Silver Nanoparticles from Eggshells, Int. J. of Pharm. Sci., 2025, Vol 3, Issue 6, 1420-1423. https://doi.org/10.5281/zenodo.15614392
10.5281/zenodo.15614392
