Matoshri Miratai Aher College of Pharmacy, Karjule Harya
Since the dawn of human civilization, plants have been a significant source of medicines. Achyranthes Aspera Linn. has been the subject of numerous published literature reviews. According to Sanskrit synonyms, Achyranthes Aspera is a rough-flowered stalk. Terpenoids, alkaloids, steroids, saponins, and flavonoids are just a few of the numerous chemical components found in Achyranthes aspera. Boils, diarrhea, dysentery, hemorrhoids, rheumatic pains, itches, and skin eruptions are all treated with the plant's juice. In Ayurveda, Achyranthes aspera Linn is referred to as Apamarga. Achyranthes Aspera is a widely accessible herb that has been used in Ayurvedic, Siddha, and Unani medicine systems to treat a variety of conditions, including arthritis, asthma, heart disease, dermatological conditions, gynecological disorders, kidney stones, leprosy, wounds, snakebite, dysentery, malaria, pneumonia, rabies, and toothpaste.In the early stages of asthma, a dried leaf powder combined with honey is helpful. Achyranthes aspera Linn., also called Agadha in Marathi and Chirchira in Hindi, is a native herb of India. It is the fundamental ingredient in a lot of conventional treatments. Numerous activities, including antifertility, antihyperlipidemic, antidiabetic, immunomodulatory, anticarcinogenic, diuretic and cardiotonic, anti-inflammatory, antifungal, and antibacterial activity, have been reported for the herb. The methanolic and dichloromethane extracts' antioxidant capacity was evaluated in vitro using the free radical DPPH. Each extract showed distinct antioxidant and antibacterial properties, which varied depending on the solvent and were dependent on time and concentration.
In India, Achyranthes aspera Linn., a member of the Amaranthaceae family, is frequently found as a weed along roadsides and in waste areas. It is referred to as Prickly Chaff Flower in English, Aghedo and Aghedi in Gujarati, Chirchira and Chirchitta in Hindi, and Apamarg in Sanskrit. Since the dawn of humanity thousands of years ago, nature has provided therapeutic substances. Since the beginning of civilization, medicinal plants have been an essential part of human life, helping to alleviate suffering. The Indian medical system is among the oldest organized medical systems and has a long history. Ayurveda, Siddha, Unani, and homeopathy are its primary influences. In India and other places, Achyranthes aspera is a common weed that grows along field edges, roadsides, and abandoned areas at altitudes of up to 2,100 m. Its presence is not limited to India but can also be found in Australia, Bangladesh, South Andaman islands, America, Ceylon, Africa and various parts of tropical Asia.
There are seven species of Achyranthes:
Taxonomy and Distribution:
METHODOLOGY:
To find potentially important scientific research and reports of Achyranthes aspera Linn, a thorough literature review was conducted using scientific databases such as Google, Google Scholar, PubMed, Web of Science, Science Direct, SciFinder, Springer Link, and Flora of China. Relevant keywords such as A. aspera, antioxidant, antimicrobial, phytochemistry, and pharmacological activities were combined. There were no linguistic limitations. The literature review contained more than 350 scientific publications. However, after elimination, the focus was restricted to 144 papers. The chemical structures were drawn using ChemDraw Professional 16.0 software.
MORPHOLOGY:
The annual herb Achyranthes aspera is used all over the world for its therapeutic qualities. Simple leaves are 1-3 feet from the stem; stamens are double-shaped; stomata are animosities; embryology is visible; anther is of the indorse type; and there are numerous covering structures. There are also medullar and vascular bundles, as well as cambium. Root: Shape of a Cylinder Its diameter is 1.0 cm. separated into secondary and tertiary roots. Simple, ovate, opposite, velvety, elliptical leaves. Flowers: spike-shaped, bracteolate, green or red. Petals: Two green or white spiked petals. Fruits: dry, utricle-stored fruits. Alnuminous seeds have a smooth, curved embryo. Androecium-5 corolla-lobed stamens. Gynoecium: superior ovary with two syncarpous.
MATERIAL AND METHODS
COLLECTION OF PLANTS -
The plant was taken from Rehman Garden in Lahore, Punjab, Pakistan, in September 2018. Dr. Zaheer-ud-Din Khan, a professor in the botany department at Government College University Lahore, verified it. The plant specimen was sent to the herbarium along with a voucher specimen bearing the number 3590.
Fig. 1a Achyranthes aspera with juicy leaves and young flowers. The plants were grown in the outdoor facility.
PREPARATION OF AQUEOUS PLANT EXTRACT-
Aqueous plant extract preparation involved cleaning, shade-drying, and coarsely powdering the chosen plant material after it had been properly identified. After thoroughly combining the granulated powder with six times the volume of water, the mixture was boiled and constantly stirred until the volume was reduced to one-third. After the extract was filtered through muslin cloth, the filterate was evaporated in a water bath until it had the consistency of a thick paste. The extracted paste was kept at 4°C in an airtight container. It was discovered that the aqueous extract had an extractive efficiency of 27.3% W/W. This extract was then used in in-vivo anti-obesity studies, the pancreatic lipase inhibition assay, and the determination of plasma triacylglycerol following oral administration of lipid emulsions to rats.
CHEMICALS :
DPPH was acquired from Sigma Aldrich Ltd. in Mumbai, and Muller Hinton agar media no. 173 was acquired from Hi media Pvt. Ltd. in Mumbai. The solvents utilized are AR Grade and were distilled prior to use. Clinical isolates of gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) bacteria were obtained from the Department of Microbiology at Auxilium College in Vellore. Experiments on animals In accordance with the guidelines set forth by the Institutional Animal Care User Committee (IACUC number 11-005), every in vivo study involving athymic nude mice was carried out utilizing a University of Miami-approved protocol.
ANIMAL EXPERIMENTS -
In accordance with the rules and regulations of the Institutional Animal Care User Committee (IACUC number 11-005), all in vivo research on athymic nude mice was carried out utilizing a University of Miami-approved protocol.
PHARMACOLOGICAL STUDIES -
ANTI INFLAMMATORY ACTIVITY -
Anti-inflammatory action Achyranthes aspera alcohol extract demonstrated anti-inflammatory properties in models of cotton pellet granuloma and carrageenan-induced hind paw oedema in albino male rats. Additionally, it has been reported that A. aspera ethanolic extract has anti-inflammatory and anti-arthritic properties at doses of 100–200 mg/kg. Rats with carrageenan-induced paw oedema, granuloma pouch, formalin-induced arthritis, and adjuvant arthritis were used to test the anti-inflammatory and anti-arthritic properties of the water-soluble alkaloid achyranthine that was extracted from A. aspera. Although it was less effective than phenylbutazone and betamethasone, it demonstrated notable anti-inflammatory activity in each of the four models used. Additionally, achyranthine increased the amount of cholesterol and ascorbic acid in the adrenal gland and significantly decreased the weight of the spleen, thymus, and adrenal gland.
ANTI- MICROBIAL ACTIVITY -
Both the whole plant of A. aspera and the aqueous solution of the base achyranthine exhibited antibacterial activity against Bacillus typhosus, Streptococcus heamolyticus, and Staphylococcus aureus. However, the leaves' alcoholic and aqueous extracts demonstrated antibacterial activity against E. coli and S. aureus. Salmonella typhimurium, Pseudomonas cichorii, and B. subtilis bacterial strains were all susceptible to the antibacterial activity of the seeds growing on cattle dung. In a different study, B. subtilis and S. aureus bacterial strains were inhibited by an 80% ethanolic extract of the plant's leaves and stem at a concentration of 25 mg/ml.
ANTI FERTILITY -
The plant has been shown to have a stronger antifertility activity in a number of experiments. In mice, extracts from different plant parts demonstrated an abortifacient effect, with benzene extract exhibiting the highest level of activity. It has been reported that the plant's aerial parts prevent female rats from becoming pregnant. The plant's leaf, root, and seed extracts regulate postpartum hemorrhage, placental retention, and fertility. It was discovered that a stem bark benzene extract had abortifacient properties in rats. The root's ethanolic extract demonstrated spermicidal activity in both in vitro and in vivo investigations.
ANTI- DIABETIC ACTIVITY -
Methanol and aqueous extracts of the ground whole plant of A. Hypoglycemic activity was demonstrated by aspera. Following oral administration of different doses, the blood glucose levels of both normal and Alloxan-induced diabetic rabbits were measured 30. The extract of A in ethanol. Aspera seed significantly reduced blood sugar levels in rats with streptozotocin-induced diabetes. The aqueous and methanol extracts of the powdered whole plant of A were reported by Akhtar et al. (1991). Aspera has demonstrated hypoglycemic action. Following oral administration of different doses, the blood glucose levels of both normal and Alloxan-induced diabetic rabbits were measured.
DIURETIC ACTIVITY -
In comparison to untreated rats, mice given 10 to 20 mg/kg intramuscularly (Lm) of the saponin derived from A. aspera seeds showed noticeably higher urine production at 2, 6, and 24 hours. Interestingly, a diuretic effect of similar magnitude was also induced by mersalyl at a concentration of 3 mg/kg. 10 mg/kg was found to be the ideal saponin dosage. Additionally, rats given oral doses of the saponin (5–10 mg/kg) showed a significant increase in urine production, similar to what was seen with acetazolamide at 10 mg/kg. Crucially, the saponin's diuretic effects were similar to those of acetazolamide and were accompanied by an increase in potassium and sodium excretion in the urine.
CARDIOVASCULAR ACTIVITY –
Achyranthine, a water-soluble alkaloid that was extracted from A. aspera, dilated blood vessels, raised the rate and amplitude of breathing in frogs and dogs, and lowered heart rate and blood pressure. Acetylcholine (0.1 mg/ml) has been shown to have a greater contractile effect on frog rectus muscles than achyranthine (0.5 mg/ml), and tubocurarine did not block its spasmogenic effect.
THERAPEUTIC USES MENTIONED IN AYURVEDIC PHARMACOPΟΕΙΑ -
The dried plant is used in sula (colic), udararoga (abdominal diseases), apaci (cervical lymphadenitis), arsa (haemorrhoids), kandu (itching), and medroga (obesity). Chardí (vomiting), adhmana (tympanitis), kandu (itching), sula (colic), apaci (lymphadenitis), granthi (tumor), bhagandara (fistula-in-ano), hrdaroga (heart disease), jwara (pyrexia), switra (leucoderma), vadhirya (deafness), udararoga (abdominal diseases), yakrtroga (liver disorders), dantaroga (tooth disease), and raktavikara (blood disorders).
THERAPEUTIC USES AS DEPICTED BY ETHNOBOTANICAL STUDIES -
The plant is used as a diuretic, astringent, and purgative for dropsy, piles, skin eruptions, colic, fractured bones, whooping cough, respiratory issues, asthma, leucoderma, and as an antidote to snake bites. The inflorescence is employed in hydrophobia and coughing. In hydrophobia, fruit is used. The seeds are used to treat gonorrhea, insect bites, hydrophobia, whooping cough, and as an anti-asthmatic. They are also used as an emetic, purgative, and cathartic. The leaves are used for wounds, injuries, typhoid, intermittent fever, urination, dog bites, and as an anti-asthmatic. The root is used as an anti-asthmatic, diuretic, diaphoretic, and antisyphilitic for whooping cough, tonsilitis, hemorrhage, cough, and hydrophobia.
MEDICINAL USES -
Fig 1b. Achyranthes Aspera with maturing seeds. The were grown in the outdoor facility.
PHYTOCHEMICAL AND PHARMACOLOGICAL REVIEW-
RESULTS -
Thyroid function and other parameters were found to be significantly altered by Achyranthes aspera extract at a dose of 200 mg/kg body weight, which is almost equivalent to 3 g/kg body weight of dry powder, as used previously by Akhtar and Iqbal (1991). When compared to the control group, the treated animals showed a significant increase in body weight, hepatic protein content, and serum glucose levels. Following the administration of the plant extract, there was also a notable rise in serum T and T concentrations as well as the T/T ratio. Hepatic lipid peroxidation (LPO) dramatically decreased in treated animals, despite a non-significant increase in hepatic superoxide dismutase (SOD) and catalase (CAT) activities.
CONCLUSION
Ayurveda may place a high value on pharmaceuticals and, second, on doctors who treat patients. Achyranthes aspera is a herb that can grow anywhere in the world and is a member of the Amaranthaceae family. referred to as Apamarga in the past. A. aspera can be studied in its entirety and found to be a widely used medicinal plant.
ACKNOWLEDGMENT-
The authors are sincerely thankful to the Department of Pharmacognosy and Phytochemistry, [Matoshri Miratai Aher College of Pharmacy, Karjule Harya.], for providing the necessary facilities and encouragement to carry out this comprehensive review work. The authors also acknowledge the guidance and valuable suggestions from faculty members of the Department of Pharmaceutics during the preparation of the manuscript.
The authors are grateful to the library and online resources that facilitated the collection of literature and reference materials related to Achyranthes Aspera and its pharmacological profile.
Finally, the authors extend their appreciation to their peers and mentors for their constructive comments and continuous support throughout the completion of this review article.
REFERENCES
Pratiksha Kardile, Amruta Varpe, Rohan Salve, Dipali Devkate, Sanika Palange, Gayatri Khodade, Dr. Rahulkumar Rahane, An Extensive Analysis of Achyranthes Aspera's (Amaranthaceae) Traditional Applications, Chemical Makeup, and Pharmacological Characteristics, Int. J. of Pharm. Sci., 2025, Vol 3, Issue 12, 157-164. https://doi.org/10.5281/zenodo.17778111
10.5281/zenodo.17778111
