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University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India
A simple, accurate, precise, sensitive and cost-effective spectrofluorimetric method was developed and validated for the estimation of quercetin (quercetin) in bulk and formulation. The relative fluorescence intensity of quercetin was measured in distilled water at an excitation wavelength of 444 nm and an emission wavelength of 538 nm. Proposed method was found to be linear over the range of 100 to 4000 ng/ml with correlation coefficient 0.9999. Proposed method was validated using different analytical method validation parameters viz. Accuracy, precision, LOD, LOQ, robustness and ruggedness using QC standards as per the ICH guidelines. The percentage recovery was found to be 100.01 % and percentage RSD values were found to be less than 2 for accuracy and precision studies. The detection and quantification limits for the proposed method were found to be 4.38 and 13.29 ng/ml, respectively. A simple, accurate, precise, sensitive yet cost-effective spectrofluorimetric method was developed for the estimation of quercetin in bulk and formulation. The said spectrofluorimetric method was found to be economic as it comprises water as a solvent.
Sachin Bhusari*, Akshay Thorat, Pravin Wakte, Development and Validation of a Spectrofluorimetric Method for the Estimation of Rifabutin in Bulk and Formulation, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 11, 161-167. https://doi.org/Sachin Bhusari*, Akshay Thorat, Pravin Wakte, Development and Validation of a Spectrofluorimetric Method for the Estimation of Rifabutin in Bulk and Formulation, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 11, 161-167. https://doi.org/10.5281/zenodo.10083916
10.5281/zenodo.10083916