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  • Development and Validation of a Spectrofluorimetric Method for the Estimation of Rifabutin in Bulk and Formulation
  • University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India

Abstract

A simple, accurate, precise, sensitive and cost-effective spectrofluorimetric method was developed and validated for the estimation of quercetin (quercetin) in bulk and formulation. The relative fluorescence intensity of quercetin was measured in distilled water at an excitation wavelength of 444 nm and an emission wavelength of 538 nm. Proposed method was found to be linear over the range of 100 to 4000 ng/ml with correlation coefficient 0.9999. Proposed method was validated using different analytical method validation parameters viz. Accuracy, precision, LOD, LOQ, robustness and ruggedness using QC standards as per the ICH guidelines. The percentage recovery was found to be 100.01 % and percentage RSD values were found to be less than 2 for accuracy and precision studies. The detection and quantification limits for the proposed method were found to be 4.38 and 13.29 ng/ml, respectively. A simple, accurate, precise, sensitive yet cost-effective spectrofluorimetric method was developed for the estimation of quercetin in bulk and formulation. The said spectrofluorimetric method was found to be economic as it comprises water as a solvent.

Keywords

Spectrofluorimetry, Rifabutin, Validation, Excitation, Emission

Reference

  1. Sachin Bhusari*, Mahesh Rindhe, Avinash Chaudhary, Pravin Wakte, Development and Validation of UV-Visible Spectrophotometric Method for Estimation of Darifenacin Hydrobromide in Bulk and Formulation, Asian Journal of Biochemical and Pharmaceutical Research, Issue 4(Vol. 8) 2018 ISSN: 2231-2560.
  2. Sachin Bhusari*, Ansari Irfan Aamer, Pravin Wakte, Development and Validation of UV-Visible Spectrophotometric Method for Estimation of Rifabutin in Bulk and Formulation, International Journal of Pharmacy and Biological Sciences, (2019) 9 (1): 1157-1162.
  3. Sachin Bhusari*, Tejal Chaudhari, Pravin Wakte, Development and validation of a spectrofluorimetric method for the estimation of camptothecin in bulk and formulation,
  4. Journal of Drug Delivery and Therapeutics, 2019.
  5. Sachin Shivling Bhusari, Bharti Gotmare, Pravin Wakte, Development And Validation of Spectroflurometric Method For the Estimation Picroside II in Picrorhiza Kurroa Extracts : Application in Standardization, Asian Journal Of Research in Chemistry and Pharmaceutical Sciences, 7(1),2019,206-213.
  6. Dr. Sachin Shivling Bhusari, Irfan Ansari, Pravin Wakte, Development and Validation of High-Performance Thin Layer Chromatography Method for Estimation of Rifabutin in Bulk and Formulation, Asian Journal of Pharmaceutical Analysis,10(1): Jan.-Mrach, 2020.
  7. Note for guidance on validation of analytical procedures: text and methodology. European medicines agency, 1995: 1-15.
  8. Validation of analytical procedures: text and methodology Q2 (R1), 1994. ICH   harmonized tripartite guideline

Photo
Sachin Bhusari
Corresponding author

University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India

Photo
Akshay Thorat
Co-author

University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India

Photo
Pravin Wakte
Co-author

University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India

Sachin Bhusari*, Akshay Thorat, Pravin Wakte, Development and Validation of a Spectrofluorimetric Method for the Estimation of Rifabutin in Bulk and Formulation, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 11, 161-167. https://doi.org/Sachin Bhusari*, Akshay Thorat, Pravin Wakte, Development and Validation of a Spectrofluorimetric Method for the Estimation of Rifabutin in Bulk and Formulation, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 11, 161-167. https://doi.org/10.5281/zenodo.10083916

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