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Abstract

Iron deficiency anemia remains a major global public health concern, particularly affecting women, children, and vulnerable populations due to inadequate nutritional intake and poor iron bioavailability. This study focuses on the formulation and evaluation of a hemoglobin-boosting herbal biscuit as a functional food approach to combat anemia. The developed biscuit incorporates natural, iron-rich and nutrient-dense ingredients such as wheat flour, ragi, dates, apricot powder, sunflower seeds, jaggery, and tulsi, aimed at enhancing hemoglobin levels and improving overall nutritional status. The preparation involved standard baking procedures followed by physicochemical and textural evaluation, including parameters such as average weight, thickness, diameter, hardness, moisture content, ash value, and color. The formulated biscuits exhibited desirable organoleptic and physical characteristics, including moderate crispness, uniform structure, acceptable moisture content (5–7%), and good mineral composition (ash value 2–3%). The results suggest that the herbal biscuits possess suitable nutritional and functional properties, making them a promising, cost-effective dietary intervention for managing iron deficiency anemia. The incorporation of bioactive compounds and iron-rich ingredients enhances both the therapeutic value and consumer acceptability of the product. Thus, this study supports the potential use of functional foods as a sustainable strategy to reduce anemia prevalence and improve public health outcomes.

Keywords

Anemia, Iron deficiency anemia (IDA), vulnerable populations, reduce anemia prevalence, hemoglobin

Introduction

The word "Anemia" (derived from the ancient Greek anemia , which means "lack of blood") refers to a collection of disorders caused by body tissues’ inability to maintain a normal haemoglobin concentration due to insufficient supply of one or more nutrients, which lowers the total amount of circulating hemoglobin.

It is one of the most prevalent nutritional disorders worldwide, particularly affecting developing countries and vulnerable populations such as children, adolescent girls, and pregnant women. According to global estimates, a significant proportion of the population suffers from anemia, with iron deficiency being the most common underlying cause

Iron deficiency anemia (IDA) arises due to inadequate dietary intake, poor absorption, or excessive loss of iron, leading to reduced hemoglobin synthesis and diminished oxygen-carrying capacity of blood. This condition manifests through symptoms such as fatigue, weakness, dizziness, and decreased cognitive and physical performance, thereby impacting overall quality of life and productivity

Despite various medical interventions, anemia continues to persist as a major public health challenge, especially in low- and middle-income countries.

Biscuit

In recent years, there has been increasing interest in the development of functional foods as a sustainable and cost-effective approach to combat nutritional deficiencies. Functional foods are enriched with bioactive compounds and essential nutrients that provide health benefits beyond basic nutrition. Incorporating iron-rich and nutrient-dense ingredients into commonly consumed food products offers a practical strategy to improve dietary intake and prevent anemia

Biscuits are widely accepted, convenient, and shelf-stable food products that can serve as an ideal vehicle for nutrient fortification. The formulation of herbal biscuits using natural ingredients such as whole wheat, ragi, dates, apricot, sunflower seeds, and tulsi provides a rich source of iron, vitamins, and antioxidants that support hemoglobin synthesis and overall health. These ingredients not only enhance nutritional value but also improve palatability and consumer acceptability.

Therefore, the present study focuses on the formulation and evaluation of hemoglobin-boosting herbal biscuits as a functional food aimed at preventing and managing iron deficiency anemia. This approach emphasizes the use of natural, affordable, and nutritionally enriched ingredients to develop a product that can contribute to improving hemoglobin levels and reducing the prevalence of anemia among vulnerable populations.

Advantages of hemoglobin boosting herbal biscuits

  • Improves hemoglobin levels
  • Prevents iron deficiency anemia
  • Natural and herbal composition, which support overall health without synthetic additives.
  • Good source of essential nutrients
  • Convenient and ready-to-eat, making it suitable for daily dietary supplementation.
  • Affordable and nutrient-dense, making it suitable for populations with limited access to balanced diets.
  • Supports child growth and development

MATERIALS

Table no 1:-Materials and their functions

Sr.no

Material

Role in formulation

1

Wheat flour

Provides structure, energy, and small amount of iron

2

Ragi flour

Rich in iron and calcium; supports hemoglobin formation

3

Apricot powder

Source of iron and vitamin C; enhances iron absorption

4

Dates powder

Provides iron and natural sugars; boosts energy and reduces fatigue

5

Sunflower seeds

Contains iron, vitamins, and healthy fats; supports blood health

6

Tulsi powder

Improves iron absorption and protects RBCs with antioxidants

7

Cardamom powder

Enhances flavor and aids digestion

8

Jaggery

Sweetening agent Rich in iron; helps increase hemoglobin levels

9

Butter

Improves texture, taste, and palatability

10

Baking powder

Acts as leavening agent; makes biscuits light and porous

11

Salt

Enhances taste and balances flavor

12

Water

Helps in dough formation and uniform mixing

METHODS AND EVALUATION

method of preparation

  • Apricots and dates were thoroughly washed, deseeded, and cut into small pieces, followed by drying in a hot air oven at 50–60°C for 1 hour or sun dried.
  • After complete drying, the apricots and dates were finely ground using a grinder and sieved to obtain a uniform powder
  • Sieve all powders to remove lumps and obtain uniform particle size.
  • All dry ingredients, including whole Wheat flour, Ragi flour, date powder, Apricot powder, Baking powder, Cardamom powder, Tulsi powder, and salt, were accurately weighed, transferred into a clean mixing bowl, and thoroughly blended to obtain a uniform dry mixture
  • Water was added gradually to the dry mixture, followed by gentle mixing and kneading to obtain a soft and smooth dough.
  • The dough was kept aside for 20-30 minutes to improve hydration and consistency.
  • The dough was rolled into a thin sheet (about 3–5 mm thick) and cut into desired shapes using a biscuit cutter
  • Sunflower seeds and apricot seeds were added after shaping biscuit to enhance its nutritional value and to enhance the visual appearance of the final product.
  • The oven was preheated to 160–180°C, and the prepared biscuits were placed on a baking tray lined with butter paper and baked for 15-20 minutes until they became light brown and crisp
  • The biscuits were removed from the oven and allowed to cool at room temperature to make them crisp.
  • Store the biscuits in an airtight container to maintain their quality and freshness.

Formulation Table

Table no.2 :-Formulation table for haemoglobin boosting herbal biscuit

Sr. No.

Ingredients

Quantity (gm) Batch 1

Quantity (gm) Batch 2

Quantity (gm) Batch 3

1

Whole wheat flour

25

30

30

2

Ragi flour

5

5

5

3

Apricot powder

1

2.5

2.5

4

Dates powder

4

5

5

5

Cardamom powder

1

0.5

0.5

6

Tulsi

0.2

0.2

0.2

7

Sunflower seeds

1

1

1

8

Jaggery

5

15

15

9

Baking powder

0.25

0.25

0.25

10

Butter

5

15

15

11

Salt

0.5

1

1

12

Water

27 ml

35 ml

35 ml

Evaluation parameters

1. Texture profile analysis /Textural characteristics of biscuit

    

 

Fig no.1:- final product

  • Smooth and slightly cracked surface, indicating proper baking
  • apricot seeds  and sunflower seeds pieces gives a slightly rough and uneven top layer.
  • Small visible pores suggest a uniform internal structure
  • Appears moderately crisp on the outside due to golden-brown colour.
  • Likely to have a slightly firm bite, not too hard
  • Looks low in moisture, which is typical for biscuits, contributing to longer shelf life.
  • Nut inclusions enhance crunch and sensory appeal.

2. Average weight:- The weight was measured in gram by analytical balance machine

Fig no.2:- Average weight

Average weight = 10.2+8.2+10.2+12.7+12.3+10.8

                            = 64.4/6

                            = 10.7 gm

3. Thickness:- The thickness was measured in mm by digital vernier caliper

                 = 4.3

Fig no.3:- Thickness

4.Diameter :- The diameter was measured in mm by digital vernier caliper

                 = 8.7

Fig no.4:- Diameter

5. Hardness:-

Measured using a hardness meter or texture analyzer to evaluate chewiness and texture.

Hardness is defined as the maximum force required to break or compress the biscuit. It indicates the texture quality of the biscuit, such as crispness or softness.

Fig no.5 :- Hardness Tester

6. Moisture content

Determination of amount of water in the sample is called moisture content. 6- cookies are prepared from which 1 is taken out for calculating initial weight, and after air-drying of this same cookie the final weight is calculated.

Moisture content in sample can determine by Oven drying method. Moisture content (%) = [(X+Y)-Z / Y] 100

Where, X=Weight in gram of empty dish.

 Y=weight in gram of sample.

 Z= weight of in gram of the dish with material after drying to constant weight.

=5-7%

7. Ash value

Ash value is important for detecting purity and quantity of total amount of the minerals and thier concentration in formulated samples. Total ash content was determined by Muffle furnace.

Total ash content (% by weight) = weight of ash / total weight of sample

                                                          = 2-3%

8. Colour determination

The prepared biscuits exhibit a uniform light to medium brown colour with a slightly golden-brown surface. This coloration is characteristic of proper baking.

RESULTS

Texture profile analysis :- revealed that the biscuits possessed a smooth and slightly cracked surface, indicating proper baking. The presence of apricot seeds and sunflower seed pieces contributed to a slightly rough and uneven surface, enhancing the product’s sensory appeal. They appeared moderately crisp externally with a firm but not hard bite, and the low moisture nature indicated good shelf stability.

Average weight:- of the biscuits was calculated using an analytical balance and was found to be 10.7 g, indicating uniformity in size and preparation.

Thickness :- was measured using a digital Vernier caliper and were found to be  4.3 mm confirming consistency in shaping and processing.

Diameter:- was measured using a digital Vernier caliper and were found to be 8.7 mm .

Hardness test :- was measured using a haedness tester and were found to be 8.7 mm indicated that the biscuits possessed moderate hardness, reflecting a desirable balance between crispness and firmness. This suggests good textural quality suitable for consumption.

Moisture content:- of the biscuits was found to be in the range of 5–7%, which is within the acceptable limit for biscuits and contributes to extended shelf life and prevention of microbial growth.

Ash value :-was determined to be 2-3%, indicating the presence of an appropriate amount of mineral content in the formulated biscuits.

Colour determination :-showed that the biscuits had a uniform light to medium brown colour with a golden-brown surface, confirming proper baking and desirable appearance

SUMMARY

The project aims to develop a hemoglobin-boosting herbal biscuit using natural ingredients like dates, sunflower seeds, apricot powder, and whole wheat to combat anemia in vulnerable populations. The biscuit is formulated to provide bioavailable iron and essential nutrients like vitamin C and folic acid. The study involves evaluating the biscuit physicochemical, nutritional, and therapeutic properties, including texture, color, sensory evaluation, and shelf life. A feeding experiment was conducted to assess the biscuit's efficacy in improving hemoglobin levels and iron status in individuals with iron deficiency anemia.

CONCLUSION

The formulated herbal biscuit shows promise as a nutritious and convenient food product to address anemia in vulnerable populations, such as women and children. The biscuit's iron-rich herbal ingredients, including apricot, dates, and sunflower seeds, provide a natural and bioavailable source of essential nutrients. The evaluation of the biscuit's physical, nutritional, and therapeutic properties demonstrates its potential as a functional food product. Nonetheless, this innovative approach can help alleviate anemia-related health issues in resource-constrained communities.

REFERENCES

  1. Kolarš B, Mijatovi? Jovin V, Živanovi? N, Minakovi? I, Gvozdenovi? N, Dickov Kokeza I, Lesjak M. Iron deficiency and iron deficiency anemia: a comprehensive overview of established and emerging concepts. Pharmaceuticals. 2025;18:1104.
  2. Lei MQ, Sun LF, Luo XS, Yang XY, Yu F, Chen XX, Wang ZM. Distinguishing iron deficiency anemia from thalassemia by the red blood cell lifespan with a simple CO breath test: a pilot study. J Breath Res. 2019;13(2):026007.
  3. Freeman AM, Zubair M. Anemia screening. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2026. Updated 2025 Feb 17.
  4. Wagh D, Kanase S, Balid A, Fulari S, Bhosale A, Wadkar S, et al. A brief review on anemia. Int J Sci Res Tech. 2024;1(12):116–125.
  5. Suprapti E, Hadju V, Ibrahim E, Indriasari R, Erika KA, Balqis B. Anemia: etiology, pathophysiology, impact, and prevention: a review. Iran J Public Health. 2025;54(3):509–520.
  6. Harper JL, Marcel EC, Emmanuel CB. Iron deficiency anemia: practice essentials, pathophysiology and etiology. Medscape. 2015.
  7. Kaur S. Iron deficiency anemia (IDA): a review. Int J Sci Res. 2016.
  8. Turner MR, Talbot K. Functional vitamin B12 deficiency. Pract Neurol. 2009;9(1):37–41.
  9. Habtie TE, Zemariam AB, Dagnaw BW, Alamaw AW, Feleke SF, Adisu MA. The immunomodulatory effect of vitamin B12 in pernicious anemia: a systematic review. Oxid Med Cell Longev. 2025;2025:8463993.
  10. Loriamini M, Cserti-Gazdewich C, Branch DR. Autoimmune hemolytic anemias: classifications, pathophysiology, diagnosis and management. Int J Mol Sci. 2024;25:4296.
  11. Hussien RS. Review of anemia: types and causes. Eur J Res Dev Sustain. 2023;4(7).
  12. Tebbi CK. Sickle cell disease: a review. Hemato. 2022;3:341–366.
  13. Sadiq IZ, Abubakar FS, Usman HS, Abdullahi AD, Ibrahim B, Kastayal BS, et al. Thalassemia: pathophysiology, diagnosis, and advances in treatment. Thalass Rep. 2024;14:81–102.
  14. Sukwuttichai P, Tidwong N, Chaipichit N, Dhippayom T, Dilokthornsakul W, Dilokthornsakul P. Global prevalence of iron deficiency anaemia among children aged 5–12 years: a systematic review and meta-analysis. J Glob Health. 2026;16:04027.
  15. Mantadakis E, Chatzimichael E, Zikidou P. Iron deficiency anemia in children residing in high- and low-income countries: risk factors, prevention, diagnosis and therapy. Mediterr J Hematol Infect Dis. 2020;12:e2020041.
  16. Castelo-Branco C, Quintas L. Iron deficiency without anemia: indications for treatment. Gynecol Reprod Endocrinol Metab. 2020;1(4):215–222.
  17. Kumar SB, Arnipalli SR, Mehta P, Carrau S, Ziouzenkova O. Iron deficiency anemia: efficacy and limitations of nutritional and comprehensive mitigation strategies. Nutrients. 2022;14:2976.
  18. World Health Organization. Iron deficiency anaemia: assessment, prevention and control. Geneva: WHO; 2001.
  19. Killip S, Bennett JM, Chambers MD. Iron deficiency anemia. Am Fam Physician. 2007;75(5):671–678.
  20. Camaschella C. Iron-deficiency anemia. N Engl J Med. 2015;372(19):1832–1843.
  21. Short MW, Domagalski JE. Iron deficiency anemia: evaluation and management. Am Fam Physician. 2013;87(2):98–104.
  22. Cappellini MD, Musallam KM, Taher AT. Iron deficiency anaemia revisited. J Intern Med. 2020;287(2):153–170.
  23. Kumar A, Sharma E, Marley A, Samaan MA, Brookes MJ. Iron deficiency anaemia: pathophysiology, assessment, practical management. BMJ Open Gastroenterol. 2022;9:e000759.
  24. Gulec S, Anderson GJ, Collins JF, et al. Mechanistic and regulatory aspects of intestinal iron absorption. Am J Physiol Gastrointest Liver Physiol. 2014;307:G397–G409.
  25. GBD 2021 Anaemia Collaborators. Prevalence, years lived with disability, and trends in anaemia burden by severity and cause, 1990–2021. Lancet Haematol. 2023;10(9):e713–e734.
  26. McCann JC, Ames BN. Iron deficiency and cognitive function: an updated review. Nutrients. 2021;13(2):447.
  27. Rahman MM, Abe SK, Rahman MS, Kanda M, Narita S, Bilano V, et al. Maternal anemia and risk of adverse birth and health outcomes in low- and middle-income countries: a systematic review and meta-analysis. Nutrients. 2022;14(2):1–15.
  28. Pasricha SR, Tye-Din J, Muckenthaler MU, Swinkels DW. Control of iron deficiency anemia: recent advances in prevention and treatment. Lancet. 2021;397(10270):233–248.
  29. Camaschella C. Iron deficiency. Blood. 2023;141(7):1–10.
  30. Skenderidou I, Leontopoulos S, Skenderidis P. Functional food ingredients enhancing immune health: a systematic review. Int J Mol Sci. 2025;26:8408.
  31. Gupta PM, Perrine CG, Mei Z, Scanlon KS. Iron, anemia, and iron deficiency anemia among young children in the United States. J Food Sci Technol. 2019;56(5):2347–2355.
  32. Hurrell R, Egli I. Iron bioavailability and dietary reference values. Am J Clin Nutr. 2010;91(5):1461S–1467S.
  33. Allen L, de Benoist B, Dary O, Hurrell R, editors. Guidelines on food fortification with micronutrients. Geneva: WHO; FAO; 2006.
  34. Krishnaswamy K, Raghuramulu N. Bioactive substances in foods. Indian J Med Res. 1998;108:257–267.
  35. Bouis HE, Saltzman A. Improving nutrition through biofortification: a review of evidence from HarvestPlus, 2003 through 2016. Glob Food Sec. 2017;12:49–58.
  36. Pareyt B, Delcour JA. The role of wheat flour constituents, sugar, and fat in low moisture cereal-based products: a review on sugar-snap cookies. Crit Rev Food Sci Nutr. 2008;48(9):824–839.
  37. Gupta RK, Gangoliya SS, Singh NK. Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains. J Food Sci Technol. 2015;52(2):676–684.
  38. Canali G, et al. Influence of different baking powders on physico-chemical and sensory properties of biscuits. J Food Sci Technol. 2020;57(10):3864–3873.
  39. Arepally D, Reddy RS, Goswami TK, Datta AK. Biscuit baking: a review. LWT Food Sci Technol. 2020;131:109726.
  40. Ayed C, Lim M, Nawaz K, et al. The role of sodium chloride in the sensory and physico-chemical properties of sweet biscuits. Food Chem X. 2021;9:100115.
  41. Pareyt B, Delcour JA. The role of water and dough rheology in baked products: a review. Compr Rev Food Sci Food Saf. 2008;7(4):345–358.
  42. Gabale BS, Phoke SV, Jaiswal K, Jaybhaye SS, Hatkar AD. Development and evaluation of a multi-herbal nutraceutical biscuit. World J Pharm Res. 2023.
  43. Binev R, Nikolov N, Bangieva D, Stoyanchev T. Effect of dietary high-protein sunflower meal on blood parameters in feedlot calves. Bulg J Vet Med. 2025;28(3):390–396.
  44. Semwal PC, Semwal A, Bhatt SP, Parashar T, Jakhmola V, Kumar S. Apricot: a source of chemically active constituents—a medicinal overview. Biomed Pharmacol J. 2023;16(2).
  45. Abdo EM, Shaltout OE-S, El-Sohaimy S, Abdalla AEM, Zeitoun AM. Effect of functional beetroot pomace biscuit on phenylhydrazine-induced anemia in albino rats. J Funct Foods. 2021;78:104385.
  46. Al-Habsi N. Date palm (Phoenix dactylifera L.) fruit: nutritional benefits and functional products. Sustainability. 2025;17:7491.
  47. Kumar BS, et al. Unleashing the nutritional power of sunflower seeds. In: Superfoods: Exploring nutrition and health benefits. 2025:99.
  48. Dhru B, Vaglela K, Zaveri M. Cardamom (Elettaria cardamomum): pharmacognosy, phytochemistry and analysis. KSV J Pharm Health Sci. 2025;2(1):11–16.
  49. Khalid A, Hameed A, Tahir MF. Wheat quality: composition, nutritional attributes and functionality. Front Nutr. 2023;10:1053196.
  50. Raturi A, Meena H, Badoni A. Finger millet (Eleusine coracana): nutritional value and agricultural importance. Asian J Adv Agric Res. 2026;26(3):1–9.
  51. Gawade DS, Patil KW, Gavit HJ. Development of value-added cookies supplemented with giloy and tulsi powder. Mater Today Proc. 2023;73:530–534.
  52. Waghmare VM, Kele VD. A review: jaggery. Int J Appl Home Sci. 2024;11(9–10):574–582.   

Reference

  1. Kolarš B, Mijatovi? Jovin V, Živanovi? N, Minakovi? I, Gvozdenovi? N, Dickov Kokeza I, Lesjak M. Iron deficiency and iron deficiency anemia: a comprehensive overview of established and emerging concepts. Pharmaceuticals. 2025;18:1104.
  2. Lei MQ, Sun LF, Luo XS, Yang XY, Yu F, Chen XX, Wang ZM. Distinguishing iron deficiency anemia from thalassemia by the red blood cell lifespan with a simple CO breath test: a pilot study. J Breath Res. 2019;13(2):026007.
  3. Freeman AM, Zubair M. Anemia screening. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2026. Updated 2025 Feb 17.
  4. Wagh D, Kanase S, Balid A, Fulari S, Bhosale A, Wadkar S, et al. A brief review on anemia. Int J Sci Res Tech. 2024;1(12):116–125.
  5. Suprapti E, Hadju V, Ibrahim E, Indriasari R, Erika KA, Balqis B. Anemia: etiology, pathophysiology, impact, and prevention: a review. Iran J Public Health. 2025;54(3):509–520.
  6. Harper JL, Marcel EC, Emmanuel CB. Iron deficiency anemia: practice essentials, pathophysiology and etiology. Medscape. 2015.
  7. Kaur S. Iron deficiency anemia (IDA): a review. Int J Sci Res. 2016.
  8. Turner MR, Talbot K. Functional vitamin B12 deficiency. Pract Neurol. 2009;9(1):37–41.
  9. Habtie TE, Zemariam AB, Dagnaw BW, Alamaw AW, Feleke SF, Adisu MA. The immunomodulatory effect of vitamin B12 in pernicious anemia: a systematic review. Oxid Med Cell Longev. 2025;2025:8463993.
  10. Loriamini M, Cserti-Gazdewich C, Branch DR. Autoimmune hemolytic anemias: classifications, pathophysiology, diagnosis and management. Int J Mol Sci. 2024;25:4296.
  11. Hussien RS. Review of anemia: types and causes. Eur J Res Dev Sustain. 2023;4(7).
  12. Tebbi CK. Sickle cell disease: a review. Hemato. 2022;3:341–366.
  13. Sadiq IZ, Abubakar FS, Usman HS, Abdullahi AD, Ibrahim B, Kastayal BS, et al. Thalassemia: pathophysiology, diagnosis, and advances in treatment. Thalass Rep. 2024;14:81–102.
  14. Sukwuttichai P, Tidwong N, Chaipichit N, Dhippayom T, Dilokthornsakul W, Dilokthornsakul P. Global prevalence of iron deficiency anaemia among children aged 5–12 years: a systematic review and meta-analysis. J Glob Health. 2026;16:04027.
  15. Mantadakis E, Chatzimichael E, Zikidou P. Iron deficiency anemia in children residing in high- and low-income countries: risk factors, prevention, diagnosis and therapy. Mediterr J Hematol Infect Dis. 2020;12:e2020041.
  16. Castelo-Branco C, Quintas L. Iron deficiency without anemia: indications for treatment. Gynecol Reprod Endocrinol Metab. 2020;1(4):215–222.
  17. Kumar SB, Arnipalli SR, Mehta P, Carrau S, Ziouzenkova O. Iron deficiency anemia: efficacy and limitations of nutritional and comprehensive mitigation strategies. Nutrients. 2022;14:2976.
  18. World Health Organization. Iron deficiency anaemia: assessment, prevention and control. Geneva: WHO; 2001.
  19. Killip S, Bennett JM, Chambers MD. Iron deficiency anemia. Am Fam Physician. 2007;75(5):671–678.
  20. Camaschella C. Iron-deficiency anemia. N Engl J Med. 2015;372(19):1832–1843.
  21. Short MW, Domagalski JE. Iron deficiency anemia: evaluation and management. Am Fam Physician. 2013;87(2):98–104.
  22. Cappellini MD, Musallam KM, Taher AT. Iron deficiency anaemia revisited. J Intern Med. 2020;287(2):153–170.
  23. Kumar A, Sharma E, Marley A, Samaan MA, Brookes MJ. Iron deficiency anaemia: pathophysiology, assessment, practical management. BMJ Open Gastroenterol. 2022;9:e000759.
  24. Gulec S, Anderson GJ, Collins JF, et al. Mechanistic and regulatory aspects of intestinal iron absorption. Am J Physiol Gastrointest Liver Physiol. 2014;307:G397–G409.
  25. GBD 2021 Anaemia Collaborators. Prevalence, years lived with disability, and trends in anaemia burden by severity and cause, 1990–2021. Lancet Haematol. 2023;10(9):e713–e734.
  26. McCann JC, Ames BN. Iron deficiency and cognitive function: an updated review. Nutrients. 2021;13(2):447.
  27. Rahman MM, Abe SK, Rahman MS, Kanda M, Narita S, Bilano V, et al. Maternal anemia and risk of adverse birth and health outcomes in low- and middle-income countries: a systematic review and meta-analysis. Nutrients. 2022;14(2):1–15.
  28. Pasricha SR, Tye-Din J, Muckenthaler MU, Swinkels DW. Control of iron deficiency anemia: recent advances in prevention and treatment. Lancet. 2021;397(10270):233–248.
  29. Camaschella C. Iron deficiency. Blood. 2023;141(7):1–10.
  30. Skenderidou I, Leontopoulos S, Skenderidis P. Functional food ingredients enhancing immune health: a systematic review. Int J Mol Sci. 2025;26:8408.
  31. Gupta PM, Perrine CG, Mei Z, Scanlon KS. Iron, anemia, and iron deficiency anemia among young children in the United States. J Food Sci Technol. 2019;56(5):2347–2355.
  32. Hurrell R, Egli I. Iron bioavailability and dietary reference values. Am J Clin Nutr. 2010;91(5):1461S–1467S.
  33. Allen L, de Benoist B, Dary O, Hurrell R, editors. Guidelines on food fortification with micronutrients. Geneva: WHO; FAO; 2006.
  34. Krishnaswamy K, Raghuramulu N. Bioactive substances in foods. Indian J Med Res. 1998;108:257–267.
  35. Bouis HE, Saltzman A. Improving nutrition through biofortification: a review of evidence from HarvestPlus, 2003 through 2016. Glob Food Sec. 2017;12:49–58.
  36. Pareyt B, Delcour JA. The role of wheat flour constituents, sugar, and fat in low moisture cereal-based products: a review on sugar-snap cookies. Crit Rev Food Sci Nutr. 2008;48(9):824–839.
  37. Gupta RK, Gangoliya SS, Singh NK. Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains. J Food Sci Technol. 2015;52(2):676–684.
  38. Canali G, et al. Influence of different baking powders on physico-chemical and sensory properties of biscuits. J Food Sci Technol. 2020;57(10):3864–3873.
  39. Arepally D, Reddy RS, Goswami TK, Datta AK. Biscuit baking: a review. LWT Food Sci Technol. 2020;131:109726.
  40. Ayed C, Lim M, Nawaz K, et al. The role of sodium chloride in the sensory and physico-chemical properties of sweet biscuits. Food Chem X. 2021;9:100115.
  41. Pareyt B, Delcour JA. The role of water and dough rheology in baked products: a review. Compr Rev Food Sci Food Saf. 2008;7(4):345–358.
  42. Gabale BS, Phoke SV, Jaiswal K, Jaybhaye SS, Hatkar AD. Development and evaluation of a multi-herbal nutraceutical biscuit. World J Pharm Res. 2023.
  43. Binev R, Nikolov N, Bangieva D, Stoyanchev T. Effect of dietary high-protein sunflower meal on blood parameters in feedlot calves. Bulg J Vet Med. 2025;28(3):390–396.
  44. Semwal PC, Semwal A, Bhatt SP, Parashar T, Jakhmola V, Kumar S. Apricot: a source of chemically active constituents—a medicinal overview. Biomed Pharmacol J. 2023;16(2).
  45. Abdo EM, Shaltout OE-S, El-Sohaimy S, Abdalla AEM, Zeitoun AM. Effect of functional beetroot pomace biscuit on phenylhydrazine-induced anemia in albino rats. J Funct Foods. 2021;78:104385.
  46. Al-Habsi N. Date palm (Phoenix dactylifera L.) fruit: nutritional benefits and functional products. Sustainability. 2025;17:7491.
  47. Kumar BS, et al. Unleashing the nutritional power of sunflower seeds. In: Superfoods: Exploring nutrition and health benefits. 2025:99.
  48. Dhru B, Vaglela K, Zaveri M. Cardamom (Elettaria cardamomum): pharmacognosy, phytochemistry and analysis. KSV J Pharm Health Sci. 2025;2(1):11–16.
  49. Khalid A, Hameed A, Tahir MF. Wheat quality: composition, nutritional attributes and functionality. Front Nutr. 2023;10:1053196.
  50. Raturi A, Meena H, Badoni A. Finger millet (Eleusine coracana): nutritional value and agricultural importance. Asian J Adv Agric Res. 2026;26(3):1–9.
  51. Gawade DS, Patil KW, Gavit HJ. Development of value-added cookies supplemented with giloy and tulsi powder. Mater Today Proc. 2023;73:530–534.
  52. Waghmare VM, Kele VD. A review: jaggery. Int J Appl Home Sci. 2024;11(9–10):574–582.   

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Shubham Kale
Corresponding author

Shraddha Institute of Pharmacy, Kondala Zambre, Washim – 444505

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Ankita Sarnaik,
Co-author

Shraddha Institute of Pharmacy, Kondala Zambre, Washim – 444505

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Dr. Swati Deshmukh
Co-author

Shraddha Institute of Pharmacy, Kondala Zambre, Washim – 444505

Shubham Kale, Ankita Sarnaik, Dr. Swati Deshmukh, Formulation and Evaluation of Hemoglobin Boosting Herbal Biscuit, Int. J. of Pharm. Sci., 2026, Vol 4, Issue 5, 949-957. https://doi.org/10.5281/zenodo.20043041

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