View Article

  • The Alternative Fungal Mounting Agent Used As The Pulp Powder Of Pomegranate In Microscopic Fungal Mounting Preparation

  • Department Of Microbiology , Yashvantrao Chavan Institute Of Science, Satara 415001 [MH] India.

Abstract

It is highly challenging to identify any unknown bacterium through microscopic examination, which is the inital step. Since there are no colour contrasts between the cells and the background when the cells are seen under a microscope because they are so small and semitransparent, the staining technique is crucial to bacteriological research since it allows researchers to see the smallest details. The LPCB is the mounting medium used for the traditional approach to fungal staining, and in the blue phenomenon, the other staining also has the property to stain fungal hyphae as a coloring agent used for general procedure, the dye, stain, or mounting medium. The following study discusses the use of pomegranate powder pulp as a natural coloring ingredient in glycerol composition to tint fungi with quality activity.

Keywords

Pomegranate pulp powder, mounting media, fungal strain, lacto phenol, cotton blue.

Reference

  1. H., Sivarajan, K., & Khubnani, A. H. (1998). Application of lactophenol cotton blue for identification and preservation of intestinal parasites in faecal wet mounts. Indian journal of pathology & microbiology, 41(2), 157–162.
  2. Thakur R. (2013). Tinea capitis in Botswana. Clinical, cosmetic and investigational dermatology, 6, 37–41. https://doi.org/10.2147/CCID.S40053
  3. Henedi, A. A., & El-Azazy, O. M. (2013). A simple technique for staining of platyhelminths with the lactophnol cotton blue stain. Journal of the Egyptian Society of Parasitology, 43(2), 419–423. https://doi.org/10.12816/0006398
  4. Saud, B., Bajgain, P., Paudel, G., Shrestha, V., Bajracharya, D., Adhikari, S., Dhungana, G., & Awasthi, M. S. (2020). Fungal Infection among Diabetic and Nondiabetic Individuals in Nepal. Interdisciplinary perspectives on infectious diseases, 2020, 7949868. https://doi.org/10.1155/2020/7949868
  5. S. C., & Prabhakar, P. K. (1995). Evaluation of lacto-phenol cotton blue for wet mount preparation of feces. Journal of clinical microbiology, 33(4), 1019–1021. https://doi.org/10.1128/jcm.33.4.1019-1021.1995
  6. Parija, S. C., & Shivaprakash, M. R. (2001). Evaluation of potassium hydroxide for wet mount preparation of stool for detection of intestinal parasites. Diagnostic microbiology and infectious disease, 41(3), 127–130. https://doi.org/10.1016/s0732-8893(01)00300-5
  7. Vignesh, R., Swathirajan, C. R., Solomon, S., Shankar, E. M., Murugavel, K. G., Paul, I., Waldrop, G., Solomon, S. S., & Balakrishnan, P. (2013). Iodine-glycerol as an alternative to lactophenol cotton blue for identification of fungal elements in clinical laboratory. Indian journal of medical microbiology, 31(1), 93–94. https://doi.org/10.4103/0255-0857.108752

Photo
Akash Ashok phudbangi
Corresponding author

Department Of Microbiology , Yashvantrao Chavan Institute Of Science, Satara 415001 [MH] India.

Akash A. Phudbangi, The Alternative Fungal Mounting Agent Used As The Pulp Powder Of Pomegranate In Microscopic Fungal Mounting Preparation, Int. J. of Pharm. Sci., 2024, Vol 2, Issue 9, 1427-1430. https://doi.org/10.5281/zenodo.13854404

More related articles
Artificial Intelligence Based Drug Designing...
Uttam Kumar , Priyal Jain, Dr. Jitendra Banweer, ...
Antidiabetic Medications: An Oral Treatment Approa...
Bhakare Mahesh, Syed A. A., Dinesh Gujrathi, Vaishnavi Siral, Son...
Gene Therapy in Cystic Fibrosis: Progress, Challen...
Sridevi Azhagammal R, Hariharan V, Midun S. A, Nathiya V, Janagan...
A Research On Design And Evaluation Of Alovera Body Lotion ...
Damini Anil Mundhare , Disha prakash wankhede , Laxmi Deshmukh, Nandakishor Deshmukh, Dr. Swati Desh...
A Review Article of An Antioxidant Activity of Moringa Oleifera Leaf...
Syed Sabreen, Dr. Dhulipalla Curie, Jetti Amos Babu, Muppuri. Venkata Gopi, Yalagala Jaya Manoj, T. ...
Related Articles
Formulation And Evaluation Of Herbal Anti-Aging Cream Using Clerodendrum Infortu...
DEVIKA K V, SANA MAJEED, MANJIMA K, SAFVANA C, SNEHA P K, VYSHNAVY DEVY D K, ...
Advances in Mucoadhesive Oral Drug Delivery: Mechanisms, Polymers, and Therapeut...
Anand Bhoyar, Amol Harsulkar, Akshay Jadhav, Aniket Kadam, ...
Molecular Detection and Drug Resistance in Multidrug-Resistant Tuberculosis (MDR...
Debobrata Sharma, Zannatul Ferdouse, Jannat Jabbar, Md. Rakibul Islam, Marjia Islam Jemi, ...
Artificial Intelligence Based Drug Designing...
Uttam Kumar , Priyal Jain, Dr. Jitendra Banweer, ...
More related articles
Artificial Intelligence Based Drug Designing...
Uttam Kumar , Priyal Jain, Dr. Jitendra Banweer, ...
Antidiabetic Medications: An Oral Treatment Approach for Regulating Blood Glucos...
Bhakare Mahesh, Syed A. A., Dinesh Gujrathi, Vaishnavi Siral, Sonal Doltade, ...
Gene Therapy in Cystic Fibrosis: Progress, Challenges, and Future Perspectives...
Sridevi Azhagammal R, Hariharan V, Midun S. A, Nathiya V, Janagan M, Jagadesh S, Naveen D, Vijayarag...
Artificial Intelligence Based Drug Designing...
Uttam Kumar , Priyal Jain, Dr. Jitendra Banweer, ...
Antidiabetic Medications: An Oral Treatment Approach for Regulating Blood Glucos...
Bhakare Mahesh, Syed A. A., Dinesh Gujrathi, Vaishnavi Siral, Sonal Doltade, ...
Gene Therapy in Cystic Fibrosis: Progress, Challenges, and Future Perspectives...
Sridevi Azhagammal R, Hariharan V, Midun S. A, Nathiya V, Janagan M, Jagadesh S, Naveen D, Vijayarag...