Vitamin C is also known as ascorbic acid. It is an antioxidant and water soluble vitamin. It is a micronutrient required for a multitude of biological functions, and is perhaps best known for its role as a terminal small-molecule antioxidant. At physiological pH, ascorbic acid most commonly exists in its mono-anion form, ascorbate. Synthesis of vitamin C (a 6-carbon lactone) from glucose occurs in the liver of many mammalian species; however, both guinea pigs and primates (e.g., humans) lack the terminal enzyme. This biosynthetic pathway (L-gulonolactone oxidase), and thus rely on dietary sources. Vitamin C performs a variety of functions that may explain why it has immune-modulating properties. Its ability to donate electrons protects biomolecules from oxidative damage caused by cell metabolism and exposure to toxins and pollutants, making it a powerful antioxidant. Vitamin C is a cofactor for enzymes involved in biosynthesis and gene regulation, including monoxygenase and dioxygenase.

Functions of vitamin C:

• Vitamin C is one of numerous antioxidants. Antioxidants are nutrients that reduce the damage caused by free radicals.
• It enhances iron absorption from plant-based sources.
• It aids the body's ability to fight infections.
• Improves bone formation.
• The body cannot produce vitamin C on its own. It does not store vitamin C. It is important to include plenty of vitamin C-containing foods in your daily diet.
• Create collagen, a crucial protein that is necessary for the formation of blood vessels, tendons, ligaments, and skin.
• All of the tissues in your body require vitamin C to develop and heal.
• It guards against heart disease and stops cholesterol from building up on artery walls.
• Collagen is the glue that keeps cells together, and it aids in the synthesis of collagen.

• Oranges -  53.2mg/100gm

• Papaya -  61mg/100gm
• Lemon -  53mg/100gm
• Amla -  300mg/100gm
• Broccoli -  89.2mg/100gm
• Guava -  228.3mg/100gm
• Red pepper -  80.4mg/100gm

Amla fruit is high in vitamin C, tannins, polyphenols, fibre, minerals, proteins, and amino acids. Research indicates that amla has hypoglycemic, anti-inflammatory, anti-hyperglycemic, anti-hyperlipidemic, and antioxidant properties, both in animals and humans.Orange is high in potassium, ascorbic acid, folate, polyphenols, flavonoids (naringin, hesperidin, neohesperidin, citronin, narirutin), and polymethoxylated flavones (tangeritin, nobilitin, sinensetin).

Lemon is a great source of vitamin C. Limonene is the primary element. . Ieaf oil was recognized with ?- pine, myrcene, neral, geranial, neryl acetate, geranyl and ?-caryophyllene. Peel oil had ?-terpinine, ?-pinene, myrcene. Modified Starch is used as a binder. When compared to pure starch-based binder, it has an appropriate viscosity and high affinity binding. Vitamin C is most inevitable essential dietary factor for human. Thus, looking towards high nutrient values of amla, lemon & oranges, we decided to formulate Vitamin C tablets & evaluate its potency by comparing it with Standard Vitamin C tablet.

MATERIALS AND METHODS:

The concept is to make vitamin c tablets by using stationary materials

• Amla
• Oranges
• Lemons

First, take 250 gm amla and cut it into pieces for four to five days before drying them in the sun. and grind these pieces to make powder. Take 300gm fresh oranges take out juice from that oranges. Take 200gm fresh lemon and take out juice from it.

Preparation of tablets: 

• Take 60gm dried amla powder prepared from 250gm amla fruit.
• Add 15ml lemon juice.
• 30ml orange juice in dried amla powder and mix it well.
• Add 1gm starch soluble powder used as binder in tablets and mix it well.
• Let it dry for about 12hrs.
• Pass this mixture through sieve to make granules.
• Compress these granules in tablet compressing machine.

RESULTS AND DISCUSSION:

The herbal vitamin C tablet has been successfully formulated and tested using IR Spectroscopy and HPLC (High Performance Liquid Chromatography).

Analysis:

The prepared vitamin C tablet was tested using IR analysis and high-performance liquid chromatography (HPLC) to identify any potential functional groups and compare its potency to a normal vitamin C tablet available in market.

FT-IR analysis:

The measurement of the absorption, emission, or reflection of infrared radiation by matter is known as infrared spectroscopy, sometimes known as vibrational spectroscopy or IR spectroscopy. It is employed in the study and identification of solid, liquid, and gaseous chemical compounds or functional groups.

Graphical interpretation:

IR spectroscopy of prepared vitamin c tablet showed frequencies (cm-1) at 3500-2800(b), 2920.80, 1740.6, 1645.40, 1470.85, 1330.14, 1241.30, 1295.23, 1120.80, 1085.18, 968.35, 765.16, 665.37, 636.60 indicating methylene, alkenes, hydroxyl, ether groups of vitamin C.