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Abstract

In compared to prior approaches, HPLC provides far higher resolution, more accurate quantitative results, and quicker analysis durations. As a result, HPLC has developed into a vital instrument in many analytical labs. Troubleshooting is a type of problem solving that is frequently used to fix failing processes or goods. Actually, the term "HPLC" is used to describe a variety of separation methods that employ a liquid mobile phase, or eluent. Understanding the fundamental principles underlying the operation of the instrument and the separation is necessary for troubleshooting HPLC equipment and separations. Component faults (pump, degasser, injector, detector, data system, column) and improper mobile phase or sample preparation are the main causes of HPLC issues. For best results, always prepare food in clean glasses. Cleaning the HPLC with the proper solvent is the primary solution to HPLC issues. It is advised to do a fast visual inspection of the instrument and column if any issue arises. Try to follow the same procedure if a monograph or other protocol is supplied. Troubleshooting is a type of issue resolution that is frequently used to fix failing processes or goods. Alternatively, we advise you to read the complete article to learn some tips that will enable you to prevent issues in the future. We hope that this article will help you detect issues and understand their underlying causes so that you can stop or lessen their occurrence in the future. The most crucial aspect of troubleshooting is the care of the colon and HPLC. We attempted to address all significant troubleshooting issues in this post.

Keywords

HPLC troubleshooting, Column Storage & Cleaning, Basic of HPLC

Reference

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Snehal Ugale
Corresponding author

Department of Pharmaceutical Quality Assurance, PRES College of Pharmacy (Women’s), Chincholi, Nashik-422102

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Vinayak Gaware
Co-author

Department of Pharmaceutical Chemistry, PRES College of Pharmacy (Women’s), Chincholi, Nashik-422102

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Rahul Khaire
Co-author

Department of Pharmaceutical Chemistry, PRES College of Pharmacy (Women’s), Chincholi, Nashik-422102

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Charushila Bhangale
Co-author

Department of Pharmaceutical Chemistry, PRES College of Pharmacy (Women’s), Chincholi, Nashik-422102

Snehal Ugale*, Vinayak Gaware, Rahul Khaire, Charushila Bhangale, A Systematic Guide On HPLC Troubleshooting, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 4, 197-209. https://doi.org/10.5281/zenodo.7883558

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