Formulation of Polyherbal Antiacne Cream

Many people prefer the herbal product over the synthetic one. Produce several harmful side effects Compare to the herbal product they have Minimal side effect. Acne becomes the major problem now a day’s in adults, it Affect Both the gender (male, female). There are two types of acne disorder which affect The individual. i.e Acne rosacea And Acne vulgaris. Acne rosacea is a typical, Constant, and medically treatable skin disorder that resembles adult acne. Acne Rosacea Typically affects the middle third of the face, particularly the nose, causing Temporary aggravation and temporary relief. The signs may appear and disappear, And the skin may remain clear for several weeks, months, or even years before They Reappear. Symptoms and indications of rosacea are: Redness of the face, tiny red Pimples and fine red lines on the Facial skin. An enormous, bulbous red nose, Eye Problems, include puffy, red eyelids and conjunctivitis.  Acne vulgaris, often known as Acne, is a common skin ailment brought on by changes in the pilosebaceous units, Which Are skin structures made up of a hair follicle and the sebaceous gland that lies Adjacent to it.[1] Acne symptoms can be Divided into three categories: mild, Moderate, and severe. Open comedons, also known as blackheads, and closed Comedons, sometimes known as white heads, are two types of non-inflammatory Lesions. Papules, pustules, cysts, and Nodules are all examples of inflammatory Lesions. Seborrhea, comedones, inflammatory lesions, the presence of bacteria In The follicular canal, and sebum production are the main characteristics of acne Vulgaris.

We have used three herbal ingredients in our preparation which are Aloe Vera gel, Neem, Tulsi. Aloe Vera gel is used as a moisturizer, to reduce pimples and acne and Also used for treatment of burn wounds. Neem is used as an antifungal and anti-Inflammatory and it is also used to reduce scar, pigmentation, redness and itching of The skin. Tulsi is used to add glow to the skin and to promote wound healing and Remove acne. 

Cream is defined as semisolid which are oil in water (o/w) or water in oil (w/o) type And these semisolid emulsions are Intended for the external application. Cream is Classified as oil in water and water in oil emulsion. It is applied on outer part or Superficial part of the skin and its main ability is to remain for a longer period of time At the site of application. The function of the Cream is to protect the skin against Different environmental condition and give soothing effect to skin and treat any Infection as well As tan and acne. There are differ#in vient types of the creams like Cleansing, cold, foundation, vanishing, massage, hand and body cream. The main Aim of our work is to develop herbal cream which can give effect like reduce acne And skin irritation, wrinkles and also Adding glow to the skin. Acne is a disturbing Issue especially for teenagers and adolescence according to global statistics Approximately 85% of Population will suffer this skin issue at age around 12-25 Years, nearly 8% adult at age 23-24 years old and only 3% of adult having Acne at Age 35-44 years old. Propionibacterium acne and staphylococcus aureus were Responsible for acne since the presence of This bacterium in the sample isolated From acne patient at the same time, the increasing number of resistance of acne Inducing bacteria Towards the antibiotic is a worrisome problem. The interest of Consumer towards the natural bioactive compound has a functional Ingredient in the Cosmetic product has arisen due to their various health beneficial effect. Beside their Nutritional and sensorial Properties also their potential effect in improving skin ihealth Has been recognized has acting as protective agent. Although, some skin issue can Categorized as self- limiting but it also can give negative psychosocial consequences To individual by having low social withdrawal due to embarrassment and at the worst Scenario it can leads to suicidal ideation. We have used to herbal ingredient in our Preparation which are Aloe Vera extract and Tulsi extract. Aloe Vera is used as Moisturizer and anti-acne. Also used for treatment of burn wound. Aloe Vera is first Active ingredient and Tulsi is second active ingredient in it. And last one is neem.

Skin

Human facial skin is a soft, flexible, and multilayered tissue that covers the face and protects underlying structures while maintaining moisture, detecting stimuli, and contributing to facial expression.

Types of skin Disease

1. Acne and Scars

Acne is one of the most common skin problems, affecting teenagers and adults alike.

It occurs due to clogged pores, excess oil production, and bacterial growth. If left

untreated, acne often leaves marks or scars on the skin.

Symptoms: Pimples, blackheads, whiteheads, inflamed red bumps, and scarring.

2. Eczema

Eczema is a chronic skin disease that causes inflammation, dryness, and itching.

Symptoms: Red, itchy, dry, or cracked skin, often found on elbows, knees, and face.

3. Psoriasis

Psoriasis is an autoimmune disease that accelerates the skin cell cycle, resulting in a

rapid buildup of cells on the surface.

Symptoms: Thick, scaly patches of

Structure of Skin

1. Aim & Objectives

Aim

To formulate and evaluate a polyherbal anti-acne cream using Aloe vera, Neem, Tulsi, and Turmeric extracts, focusing on their antimicrobial, anti-inflammatory, antioxidant, and skin-protective properties.

Objectives

• To treat acne effectively
• To provide antimicrobial activity
• To reduce inflammation and redness
• To utilize synergistic effect of herbs
• To develop a safe and natural formulation
• To improve skin condition and texture
• To formulate a stable and effective topical dosage form
• To evaluate physicochemical and biological properties

4.    MATERIALS AND METHODS 

A. Plant Profile 

1. NEEM

Synonyms : Azadirachta indica, Indian Lilac, Margosa Tree, Nim (Hindi), Kadunimb (Marathi)

Biological source : Neem consists of dried leaves, bark, seeds, and oil Obtained from Azadirachta indica, belonging to the

 

Biological Source : Dried Juice of the leaves of Aloe Barbadensis Miller

(curacao aloes), Aloe perryi Baker (socotrine aloes).

Family: Liliaceae

Chemical Constituents : There are seventy-five potentially active Ingredients in aloe vera, including vitamins, enzymes, minerals, Carbohydrates, lignin, saponins, amino acids, and salicylic acids

Uses :

• Antibacterial Activiy
• Anti-inflammatory Property
• Antiacne Properties
• Aloe vera can also be used to moisturize and soften skins 
• Aloe vera has the ability to reduce facial pigmentation and dark Patches

3. TURMERIC  

Synonyms : Curcuma domestica Valeton, Turmeric, Haldi

Scientific name : Curcuma Longa

Biological Source : Turmeric consists of the dried rhizomes (underground stems) of the plant Curcuma longa, 

Family : Zingiberaceae

Chemical Constituents :

Contains curcuminoids: curcumin, demethoxycurcumin, Bisdemethoxycurcumin  Contains volatile oil: turmerone, atlantone, zingiberene Also contains proteins, carbohydrates, resins, and starch Main active constituent: Curcumin

Uses :

• Used as an anti-inflammatory agent
• Used as an antiseptic and wound healer
• Used in skin disorders and acne treatment
• Acts as an antioxidant
• Used in digestive disorders
• Used as a natural coloring and flavoring agent

4.Tulsi

Synonym: Tulsi, Holy Basil

Biological Source: Tulsi consists of the fresh and dried leaves (and Sometimes flowering tops) of Ocimum sanctum Linn. Ocimum sanctum / Ocimum tenuiflorum

Family: Lamiaceae

Chemical Constituents :

Tulsi contains various important chemical compounds, such as:

• Eugenol – main active constituent (antiseptic, analgesic)
• Ursolic acid – anti-inflammatory property
• Rosmarinic acid – antioxidant
• Linalool – aromatic compound
• Flavonoids – apigenin, luteolin
• Tannins and saponins

Uses :

• Acts as an antimicrobial and anti-inflammatory agent
• Used in treatment of cough, cold, and fever
• Helps boost immunity

5. Methods of prepration 

Collection of plant material Neem extract , Turmeric extract , Aloe vera gel , Tulsi extract, Stearic acid , Cetyl Alcohol , Beeswax , Liquid paraffin , Glycerin g, Distilled water ,Methyl & Propyl Paraben + Rose water, Fragrance (optional).

Extraction processes

1.Extraction of Neem leaves

 Neem leaves were collected and washed with distilled water and Dried in hot air oven.

 After proper drying, leaves were powdered. 

 Then 10g Neem leaves powder in 100ml water watch taken in a Valumetric flask and shaken.

 Then the solution was heated on a water bath 80 to 100 degree Celsius in 30 minutes.

 Then the filtrate or filter product obtained, which is a clear solution

or clear extract of Neem leaves, was used in the preparation.

2.Extraction of Turmeric Powder 

Extraction of turmeric powder from Curcuma longa is a simple

Process. First, fresh turmeric rhizomes are collected and washed

Properly to remove dirt.

Then 5g Turmeric powder in 100ml water watch taken in a

Valumetric flask and shaken 

The solution was heated on water bath 80 to 100 degree Celsius in 30 minutes 

 Then the filtrate or filter product obtained, which is a clear solution

Or clear extract of Neem leaves, was used in the preparation.

Extraction of Tulsi Leaves 

• Tulsi leaves were collected and washed with distilled water and Dried hot air oven.
•  Then after proper drying, the leaves were powdered.
• Then 5g tulsi Leaves powder in a 50ml water watch taken in a Valumetric flask.
• Then the solution was heated on water bath 80 to 100 degree Celsius for 25 minutes.
•  Then filtered the solution use by filter paper and clear extract of Tulsi Leaves 

Formulation of Cream

Preperation of Oil Phase 

• Bees wax, liquid paraffin, Hard paraffin and Borax are accurately Weighted and melted in porcelain.
•  The temperature is maintain between 65°c to 75°c of oil phase.

2.Preperation of Aqueous Phase 

• Water is heated and the temperature of the phase was maintained At 65°c to 70°c 

Development of Cream Formulation 

Aqueous phase is slowly added into oily phase at 65°c to 75°c mixed for

10 to 15 minutes. The prepared herbal extract is added into the above

Part slowly when temperature reached to 40°c and mixed until it get cold.

The pH of Cream is kept between 5 to 7.

Evaluation parameters 

A. Physical Evaluation 

1.Irritancy:

Mark the area (1 cm2) on the left-hand dorsal surface. Then the cream Was applied to that area and the time was noted. Then it is checked for Irritancy, erythema, and endemic any for an interval up to 24 h and Reported.

REFERENCES

1. Saini, P.K. et al. (2025). Formulation and Evaluation of an Oil‑in‑Water Herbal Cream Containing Aloe Vera, Neem and Turmeric Extracts – This study reports the formulation of an O/W herbal cream With Aloe vera, Neem, Turmeric and evaluates stability, pH, viscosity, Spreadability and antimicrobial in vitro activity.   
2. Sharma, N., Shrivastava, P., & Tiwari, S.P. (2025). Formulation and Characterization of Herbal Cream for the Management of Acne Vulgaris This article focuses on a herbal cream including Neem, Aloe vera, Turmeric with characterization of physicochemical properties and Antimicrobial testing.  
3. Ansong, J.A. et al. (2023). Formulation and Evaluation of Herbal‑Based Antiacne Gel Preparations – Though this is a gel (not Cream), it describes phytochemical evaluation, antimicrobial assays, and Gel performance, which can support methodology and discussion of Herbal anti‑acne products.   
4. Dhyani, A., & Singh, V. (2017). Formulation and Evaluation of Multipurpose Herbal Cream – Includes Aloe vera, Neem, Turmeric Among other herbs and discusses basic evaluation such as spreadability And stability  
5. Sharma N, Sharma C, Mishra CP. Formulation and Evaluation of an Oil‑in‑Water Herbal Cream Containing Aloe Vera, Neem and Turmeric Extracts. Journal of Drug Discovery and Therapeutics. 2025;13(6).   
6. Sharma N, Shrivastava P, Tiwari SP. Formulation and Characterization Of Herbal Cream for the Management of Acne Vulgaris. Journal of Neonatal Surgery. 2025;14(25S).   
7. Ansong JA, Asante E, Johnson R, et al. Formulation and Evaluation of Herbal‑Based Antiacne cream Preparations. Biomed Research International. 2023; Article ID 7838299.
8. Dhyani A, Uttarakhand Council of Science and Technology, Singh N. Formulation and evaluation of multipurpose herbal cream. Journal of Drug Delivery and Therapeutics. 2017;9(2).   
9. Ali, B. M. M., Ghoname, N. F., Hodeib, A. A., & Elbadawy, M. A. (2015). Significance of topical propolis in the treatment of facial acne vulgaris. Egyptian Journal of Dermatology and Venerology, 35(1), 29-36.   
10. Rathore S, Gupta S, Gupta V. A Comprehensive Review on Formulation and Evaluation of Herbal Anti‑acne Cream. Journal of Cardiovascular Disease Research. 2023.   
11. Akash E Formulation and Evaluation of Anti‑Acne Herbal Cream Incorporating Aloe vera, Turmeric, Neem and Honey. International Journal of Allied Medical Sciences and Clinical Research. 2025.   
12. Shinde SC, Kumbhar S. Polyherbal Formulation of Anti‑acne Cream. Indian Pharmacy Research Journal. (Year not specified)  
13. Rathore, S., Gupta, S., & Gupta, V. (2023).A comprehensive review On formulation and evaluation of herbal anti-acne cream. Journal of Cardiovascular Disease Research, 14(2), 102–110.
14. Akash, E., Sharma, R., & Patel, D. (2025).Formulation and evaluation Of anti-acne herbal cream. International Journal of Allied Medical Sciences and Clinical Research, 13(1), 45–52.
15. Karande, S. M., Patil, P. R., & Jadhav, A. B. (2024).Formulation and Evaluation of poly-herbal anti-acne cream. Journal of Propulsion Technology, 45(3), 220–228.
16. Sucharitha, P. A. M., Reddy, K. S., & Kumar, M. (2024).Preparation And evaluation of poly herbal anti-acne cream. International Journal of Pharmacognosy and Chemistry, 6(2), 90–97.
17. Cui, H., Li, Y., & Zhang, L. (2022).Development of novel topical anti-Acne cream containing postbiotics. Indian Journal of Dermatology, 67(5), 512–518.
18. Sharma, P., & Singh, R. (2020).Formulation and evaluation of anti-Acne cream using clove oil. Materials Today: Proceedings, 33, 1234–1238.
19. Gupta, A., & Verma, S. (2025).Evaluation of anti-acne potential of Herbal and fruit extracts. Journal of Herbal Medicine, 28, 100450.
20. Kaur, H., & Kaur, G. (2021).Herbal treatment for acne vulgaris: A Review. International Journal of Pharmaceutical Sciences Review and Research, 68(1), 150–156.
21. Dhyani A, Chander V, Singh N. Formulation and evaluation of multi-Purpose herbal cream. J drug deliv ther. 2019; 9(2): 341-343. 
22. Bhowmik D, Chiranjib YJ, Tripathi KK, Kumar KS. Herbal remedies of Azadirachta indica and its medicinal application. J Chem Pharm Res. 2010; 2(1): 62-72. 
23. Sah AK, Vijaysimha M, Mahamood M. The tulsi, queen of green Medicines: Biochemistry and pathophysiology-a review. Int J Pharm sci Rev Res. 2018; 50(2): 106-114.
24. Viswanathan MV, Unnikrishnan PM, Komatsu K, Fushimi H, Basnet P. A brief introduction to Ayurvedic system of medicine and some of its Problems. Indian J Tradit Knowl. 2003; 2(2): 159-169.
25. Imhof A, Pine DJ. Stability of nonaqueous emulsions. J Colloid Inter-Face Sci. 1997; 192(2): 368-374. 
26. Mohamed MI. Optimization of chlorphenesin emulgel formulation. The AAPS J. 2004; 6(3): 81-87. 
27. Bleckmann A, Kropke R, Schneider G, Beiersdorf AG. Preparation of The w/o emulsion type with an increased water content, additionally Comprising one or more alkylmethicone copolyols and/or alkyldime-Thicone copolyols, and cationic polymers. US patent application. 2021. 
28. Kapoor S, Saraf S. Formulation and evaluation of moisturizer con-Taining herbal extracts for the management of dry skin. Pharmacog J. 2010; 2(11): 409-417. 
29. Ashara K, Soniwala MM, Paun J, Chawda J. Importance of tritura-Tion technique on preparation and evaluation of cold cream. Inventi rapid Pharm Tech. 2013: 1-2. 
30. Navindgikar N, Kamalapurkar KA, Chavan PS. Formulation and Evaluation of multipurpose herbal cream. Int J Curr Pharm Res. 2020; 12(3): 25-30. 
31. Gupta N, Dubey A, Prasad P, Roy A. Formulation and evaluation of Herbal fairness cream comprising hydroalcoholic extracts of Pleuro-tus Ostreatus, Glycyrrhiza glabra and Camellia sinensis. Pharma Bio Sci J. 2015: 40-45