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Abstract

Mosquito-borne diseases continue to be a big problem for global health, which shows the importance of finding safe and effective ways to repel mosquitoes.This study is about creating and testing a herbal mosquito repellent roll-on.Orange peel extract, which is high in limonene, was the main ingredient used.The product was made to be a natural and environmentally friendly choice compared to chemical-based repellents.Other ingredients like HPMC, glycerine, ethanol, methyl paraben, triethanolamine, and orange oil were also included.Three versions of the product (F1, F2, and F3) were made with different amounts of the main ingredient.Each version was checked for physical and chemical properties.The tests included how it looked, its pH level, thickness, how easily it spread on the skin, and whether it caused irritation.Stability tests were also done to see how well the product held up over time.The results showed that all three versions had good qualities and were stable.Among them, F3 had the best thickness and spreadability.It also worked well as a mosquito repellent without causing any skin irritation.Therefore, this developed product is safe, stable, and ready for possible use in the market

Keywords

Mosquito Repellent Roll-On, Orange Peels, Mosquito-borne diseases

Introduction

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Mosquito-borne diseases are still a big problem for public health around the world, especially in warm and humid areas.In countries like India, diseases such as malaria, dengue, chikungunya, and filariasis affect millions of people every year. As mosquitoes become less responsive to traditional chemical insecticides, and people are becoming more aware of the dangers of synthetic repellents, there is a growing need for safer, eco-friendly, and plant-based alternatives. Natural products from plants are becoming more popular because they are easy to break down, less harmful, and good for the environment.Orange peels, which are often thrown away as waste from the fruit industry, are a rich source of essential oils and other important compounds.These peels have volatile ingredients like limonene and citral that work well as mosquito repellents. Using orange peel extract is not only a cost-effective and natural way to make repellents but also helps in managing waste and gives more value to agricultural by-products.Making a mosquito repellent in the form of a roll-on has many practical benefits.Roll-ons are easy to use, do not leave a greasy feel, and let people control how much product they apply. They are portable and convenient for everyday use, especially when being outdoors.

Applying repellent directly on the skin is one of the most popular and effective ways to protect against mosquito bites.This study focuses on making and testing a mosquito repellent roll-on using orange peel extract.The product is designed to be safe, effective, and easy to use. It is tested for various qualities such as how it looks, its pH, thickness, how easily it spreads, how stable it is, whether it causes irritation, and how well it repels mosquitoes. This approach encourages the use of natural ingredients and supports sustainability by using renewable resources and reducing reliance on synthetic chemicals.

 

 

Fig no.1 Orange peels

Advantages of Mosquito Repellent Roll On Using Orange Peels

  • Uses natural and eco-friendly ingredients
  • Makes use of waste orange peels, making it cost-effective
  • Rich in limonene, which has strong mosquito-repellent properties
  • Safe for the skin compared to synthetic alternatives
  • Has a pleasant citrus scent

Definition of Mosquito Repellent:

Mosquito repellents are substances applied to the skin, clothing, or other surfaces to prevent mosquitoes from landing, biting, or feeding.These products either mask body odors or create a scent that repels mosquitoes. They are essential for preventing diseases like malaria and dengue.

  1. Material and Equipment
    1. Material

Table no 1 Material and their role

Material

Role in formulation

Fresh Orange peels

Source of natural mosquito-repellent compounds

Ethanol

Extraction of active phytochemicals

HPMC

Provides desired consistency and gel structure

Glycerin

Acts as moisturizer and Improves smooth application

Methyl Paraben

Prevents microbial contamination

Orange oil

Imparts pleasant fragrance

Triethanolamine

Adjusts and maintains pH

Distilled water

Solvent and vehicle for formulation

Roll on bottles

Enables easy, uniform and convenient application

    1. Equipment

 

Table no.2 Equipment and their purpose

Equipment

Their purpose

Grinder

Grind dried orange peels into fine powder

Maceration setup

Extract active constituents from orange peels

pH meter

Measure pH and ensure skin compatibility

Hot air oven

Dry fresh orange peels and remove moisture

 

  1. Method and Evaluation
    1. Methodology
  1. Preparation of Orange Peel Extract:
  • Fresh orange peels were collected, washed with distilled water, and dried in the sun until completely dry.
  • The dried peels were ground into a fine powder. The powder was then soaked in ethanol for 48–72 hours with regular stirring. The mixture was then filtered using Whatman filter paper.

 

 

Fig no. 2 Extraction of orange peels

  1. Preparation of Roll-On Base:
  • HPMC was mixed with distilled water, and glycerin was added as a moisturizer.
  • The mixture was stirred continuously using a magnetic stirrer until a smooth gel was formed. The pH was adjusted to 5.5–6.5 using triethanolamine.
  1. Incorporation of Extract:
  • The orange peel extract was added slowly to the gel base while stirring continuously to ensure even mixing. A small amount of methyl paraben and a few drops of orange oil were also added.
  1. Filling:
  • The final product was poured into roll-on bottles and sealed properly.

4.2 Evaluation tests:

Physical Appearance:

The final product was clear, smooth, and had a light orange color.It was free from any visible particles, clumps, or separation. The uniform texture showed that all the ingredients were well mixed. The appearance remained the same even after storage, indicating good physical stability.

pH Test:

The pH of the product was found to be around 5.6, which is safe for the skin and helps keep the product stable.This pH level is comfortable for the skin and prevents any irritation.

 

 

Fig no. 3 Digital pH meter

Spreadability:

During testing on glass slides, the product spread easily with little effort, forming a thin and even layer on the skin.This helps in the even application of active ingredients. Good spreadability improves both the effectiveness and user experience.

 

                            Fig no.4 Spreadability Test

 

Skin Irritation Test:

The product was tested on a small area of skin to check for possible irritation.No redness, itching, or swelling was observed, indicating that the ingredients are safe and mild on the skin.

 

 

 

 

Drying Test:

The product dried quickly after application, which is comfortable for the user. It did not leave a sticky or oily residue. The presence of ethanol helped speed up the drying process. Quick drying makes the product more convenient to use.

Washability Test:

The product was easily washed off with water without needing to rub hard. No residue or oily layer was left behind. This ensures better hygiene and comfort. Good washability makes the product more suitable for daily use.

 

 

Fig no. 6 Washability Test

Stability Test:

The product was stored at room temperature and checked over time. No major changes in color, smell, or texture were noticed. It remained consistent and stable during the entire study. This shows that the product has good shelf life.

Repellency Test:

The product showed effective mosquito repellent activity after application. It provided protection against mosquito bites for a reasonable period. The natural compounds like limonene in the product helped in repelling mosquitoes. Therefore, the formulation was found to be effective and reliable.

  1. Result

 

                   Fig no. 6 Washability Test

Table no. 3 Result

Sr. No.

Parameter

F1

F2

F3

1.

Colour

Pale Green

Yellow

Pale Green

2.

Odour

Unpleasant

Moderate cirus smell

Citrus smell

3.

Washability

Easily washable

Easily washable

Easily washable

4.

Skin irritation

No

No

No

5.

pH

5.1

5.4

5.6

6.

Apperance

Slightly thick

Smooth and uniform

Smooth and uniform, best consistency

7.

Overall

Not good

Better

Best

 
  1. Summary and Conclusion
  • Summary:

This study focused on creating and testing a herbal mosquito repellent roll-on made with orange peel extract, which is rich in limonene.Three different formulations (F1, F2, F3) were made using HPMC and other ingredients. Each batch was checked for appearance, pH level, how easily it spreads on the skin, whether it irritates the skin, how well it washes off, and its stability over time. The results showed that all the formulations were stable, gentle on the skin, and did not cause irritation. Among them, F3 had better spreading ability and overall performance, making it the most effective option.

CONCLUSION

The study found that orange peel extract can be used effectively as a natural and eco-friendly mosquito repellent in a roll-on form.The product was found to be safe, effective, and easy to use. Limonene in the orange peel provides good mosquito repellent effects without causing harm to the skin. The product performed well in all the tests and remained stable throughout the study. Therefore, this mosquito repellent roll-on could be a good alternative to chemical-based repellents, encouraging the use of herbal and environmentally friendly products.

REFERENCES

  1. Prabha R, Raaja V. N2. Indian Journal of Science. 2012;1(1).
  2. Curtis CF, Lines JD, Ijumba J, Callaghan A, Hill N, Karimzad MA. The relative efficacy Of Repellents against mosquito vectors of disease. Medical and Veterinary Entomology. 1987;16:109–119.
  3. Ansari MA, Razdan RK. Relative efficacy of various oils in repelling mosquitoes. Indian Journal Of Malariology. 1995;32:104Mishra P, Singh R, Verma A. Formulation and Evaluation of herbal Mosquito repellent roll-on using essential oils. Int J Pharm Sci Res. 2025;16(3):1456-1462.
  4. Patel K, Shah N, Desai P. Development of a natural mosquito repellent using orange peel Extract Rich in limonene. J Herbal Med. 2025;14(2):89-96.
  5. Rasyadi Y, Putra A, Handayani D. Formulation and evaluation of mosquito repellent roll-On Containing Citrus microcarpa peel essential oil. Asian J Pharm Clin Res. 2024;17(1):112-118.
  6. Nerio LS, Olivero-Verbel J, Stashenko E. Repellent activity of essential oils: A review. Bioresour Technol. 2010;101(1):372-378.
  7. Isman MB. Plant essential oils for pest and disease management. Crop Prot. 2000;19(8-10):603-608.
  8. Koul O, Walia S, Dhaliwal GS. Essential oils as green pesticides: Potential and constraints. Biopestic Int. 2008;4(1):63-84.
  9. Tripathi AK, Prajapati V, Aggarwal KK, Khanuja SPS, Kumar S. Repellency and toxicity Of oil From Citrus species against mosquito vectors. J Med Entomol. 2001;38(4):523-526.
  10. Choochote W, Chaithong U, Kamsuk K, Jitpakdi A, Tippawangkosol P. Repellent activity Of Selected plant essential oils against Aedes aegypti. Fitoterapia. 2007;78(5):359-364.
  11. Mishra A, Kumar S, Singh R. Development and evaluation of herbal mosquito repellent Roll-on using plant-based essential oils. J Pharm Innov. 2025;10(2):115–121
  12. Dessai P, Pangam H. Development of herbal mosquito repellent roll-on using plant-based Essential oils. Int J Res Pharm Sci. 2023;14(1):55–60
  13. Sahu B, Mishra S, Patel R. Preparation of herbal mosquito repellent dhoop using natural Plant materials. Int J Herbal Med. 2021;9(2):34–38
  14. Fradin MS. Mosquitoes and Mosquito Repellents: A Clinician’s Guide ,Ann Inter Med 1998;128:931-940.
  15. Mishra AK, Singh N and Sharma VP. Use of neem oil as a mosquito repellent in tribal Villages of mandla district, madhya pradesh, Indian J Malariol. 1995;32(3):99-103.
  16. National Institute of Malaria Research. Guidelines for mosquito control and repellent use.New Delhi: NIMR; 2021.

Reference

  1. Prabha R, Raaja V. N2. Indian Journal of Science. 2012;1(1).
  2. Curtis CF, Lines JD, Ijumba J, Callaghan A, Hill N, Karimzad MA. The relative efficacy Of Repellents against mosquito vectors of disease. Medical and Veterinary Entomology. 1987;16:109–119.
  3. Ansari MA, Razdan RK. Relative efficacy of various oils in repelling mosquitoes. Indian Journal Of Malariology. 1995;32:104Mishra P, Singh R, Verma A. Formulation and Evaluation of herbal Mosquito repellent roll-on using essential oils. Int J Pharm Sci Res. 2025;16(3):1456-1462.
  4. Patel K, Shah N, Desai P. Development of a natural mosquito repellent using orange peel Extract Rich in limonene. J Herbal Med. 2025;14(2):89-96.
  5. Rasyadi Y, Putra A, Handayani D. Formulation and evaluation of mosquito repellent roll-On Containing Citrus microcarpa peel essential oil. Asian J Pharm Clin Res. 2024;17(1):112-118.
  6. Nerio LS, Olivero-Verbel J, Stashenko E. Repellent activity of essential oils: A review. Bioresour Technol. 2010;101(1):372-378.
  7. Isman MB. Plant essential oils for pest and disease management. Crop Prot. 2000;19(8-10):603-608.
  8. Koul O, Walia S, Dhaliwal GS. Essential oils as green pesticides: Potential and constraints. Biopestic Int. 2008;4(1):63-84.
  9. Tripathi AK, Prajapati V, Aggarwal KK, Khanuja SPS, Kumar S. Repellency and toxicity Of oil From Citrus species against mosquito vectors. J Med Entomol. 2001;38(4):523-526.
  10. Choochote W, Chaithong U, Kamsuk K, Jitpakdi A, Tippawangkosol P. Repellent activity Of Selected plant essential oils against Aedes aegypti. Fitoterapia. 2007;78(5):359-364.
  11. Mishra A, Kumar S, Singh R. Development and evaluation of herbal mosquito repellent Roll-on using plant-based essential oils. J Pharm Innov. 2025;10(2):115–121
  12. Dessai P, Pangam H. Development of herbal mosquito repellent roll-on using plant-based Essential oils. Int J Res Pharm Sci. 2023;14(1):55–60
  13. Sahu B, Mishra S, Patel R. Preparation of herbal mosquito repellent dhoop using natural Plant materials. Int J Herbal Med. 2021;9(2):34–38
  14. Fradin MS. Mosquitoes and Mosquito Repellents: A Clinician’s Guide ,Ann Inter Med 1998;128:931-940.
  15. Mishra AK, Singh N and Sharma VP. Use of neem oil as a mosquito repellent in tribal Villages of mandla district, madhya pradesh, Indian J Malariol. 1995;32(3):99-103.
  16. National Institute of Malaria Research. Guidelines for mosquito control and repellent use.New Delhi: NIMR; 2021.

Photo
Shreya Rajbinde
Corresponding author

Shraddha Institute Of Pharmacy, Kondala Zambre, Washim-444505.

Photo
Swati Kanoje
Co-author

Shraddha Institute Of Pharmacy, Kondala Zambre, Washim-444505.

Photo
Dr. Swati Deshmukh
Co-author

Shraddha Institute Of Pharmacy, Kondala Zambre, Washim-444505.

Shreya Rajbinde, Swati Kanoje, Dr. Swati Deshmukh, Research On Formulation and Evaluation of Mosquito Repellent Roll-On by Using Orange Peels, Int. J. of Pharm. Sci., 2026, Vol 4, Issue 5, 1717-1722, https://doi.org/10.5281/zenodo.20082203

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